Chromosome associations in budding yeast caused by integrated tandemly repeated transgenes

Jorg Fuchs, Alexander Lorenz, Josef Loidl

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Abstract

The binding of GFP-tagged tetracycline repressor (TetR) molecules to chromosomally integrated tetracycline operator (tetO) sequence repeats has been used as a system to study chromosome behaviour microscopically in vivo. We found that these integrated transgenes influence the architecture of yeast interphase nuclei, as chromosomal loci with tandem repeats of exogenous tetO sequences are frequently associated. These associations occur only if TetR molecules are present. tetO tandem repeats associate regardless of their chromosomal context. When they are present at a proximal and a distal chromosomal position, they perturb the normal polarized Rabl-arrangement of chromosome arms by recruiting chromosome ends to the centromeric pole of the nucleus. Associations are established at G and are reduced during S-phase and mitosis. This system may serve as a model for the role of DNA sequence-specific binding proteins in imposing nonrandom distribution of chromosomes within the nucleus.
Original languageEnglish
Pages (from-to)1213-1220
Number of pages8
JournalJournal of Cell Science
Volume115
Issue number6
Publication statusPublished - 15 Mar 2002

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Keywords

  • yeast
  • chromosomes
  • nucleus
  • nuclear architecture
  • GFP
  • pairing
  • FISH
  • mitosis

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