ClassII peroxidase-encoding genes are present in a phylogenetically wide range of ectomycorrhizal fungi

Inga T. M. Bodeker*, Cajsa M. R. Nygren, Andy F. S. Taylor, Ake Olson, Bjorn D. Lindahl

*Corresponding author for this work

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Fungal peroxidases (ClassII) have a key role in degrading recalcitrant polyphenolic compounds in boreal forest wood, litter and humus. To date, their occurrence and activity have mainly been studied in a small number of white-rot wood decomposers. However, peroxidase activity is commonly measured in boreal forest humus and mineral soils, in which ectomycorrhizal fungi predominate. Here, we used degenerate PCR primers to investigate whether peroxidase-encoding genes are present in the genomes of a wide phylogenetic range of ectomycorrhizal taxa. Cloning and sequencing of PCR products showed that ectomycorrhizal fungi from several different genera possess peroxidase genes. The new sequences represent four major homobasidiomycete lineages, but the majority is derived from Cortinarius, Russula and Lactarius. These genera are ecologically important, but consist mainly of non-culturable species from which little ecophysiological information is available. The amplified sequences contain conserved active sites, both for folding and substrate oxidation. In some Cortinarius spp., there is evidence for gene duplications during the evolution of the genus. ClassII peroxidases seem to be an ancient and a common feature of most homobasidiomycetes, including ectomycorrhizal fungi. Production of extracellular peroxidases may provide ectomycorrhizal fungi with access to nitrogen sequestered in complex polyphenolic sources. The ISME Journal (2009) 3, 1387-1395; doi: 10.1038/ismej.2009.77; published online 2 July 2009

Original languageEnglish
Pages (from-to)1387-1395
Number of pages9
JournalThe ISME Journal
Volume3
Issue number12
Early online date2 Jul 2009
DOIs
Publication statusPublished - Dec 2009

Keywords

  • programs
  • degenerate primer
  • nutrient cycling
  • ectomycorrhiza
  • ecosystems
  • database
  • soil organic matter
  • C-14-labeled lignin
  • litter
  • decomposition
  • forests
  • degradation
  • degrading heme peroxidases
  • mycorrhizal fungi
  • classII peroxidases

Cite this

ClassII peroxidase-encoding genes are present in a phylogenetically wide range of ectomycorrhizal fungi. / Bodeker, Inga T. M.; Nygren, Cajsa M. R.; Taylor, Andy F. S.; Olson, Ake; Lindahl, Bjorn D.

In: The ISME Journal, Vol. 3, No. 12, 12.2009, p. 1387-1395.

Research output: Contribution to journalArticle

Bodeker, Inga T. M. ; Nygren, Cajsa M. R. ; Taylor, Andy F. S. ; Olson, Ake ; Lindahl, Bjorn D. / ClassII peroxidase-encoding genes are present in a phylogenetically wide range of ectomycorrhizal fungi. In: The ISME Journal. 2009 ; Vol. 3, No. 12. pp. 1387-1395.
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AB - Fungal peroxidases (ClassII) have a key role in degrading recalcitrant polyphenolic compounds in boreal forest wood, litter and humus. To date, their occurrence and activity have mainly been studied in a small number of white-rot wood decomposers. However, peroxidase activity is commonly measured in boreal forest humus and mineral soils, in which ectomycorrhizal fungi predominate. Here, we used degenerate PCR primers to investigate whether peroxidase-encoding genes are present in the genomes of a wide phylogenetic range of ectomycorrhizal taxa. Cloning and sequencing of PCR products showed that ectomycorrhizal fungi from several different genera possess peroxidase genes. The new sequences represent four major homobasidiomycete lineages, but the majority is derived from Cortinarius, Russula and Lactarius. These genera are ecologically important, but consist mainly of non-culturable species from which little ecophysiological information is available. The amplified sequences contain conserved active sites, both for folding and substrate oxidation. In some Cortinarius spp., there is evidence for gene duplications during the evolution of the genus. ClassII peroxidases seem to be an ancient and a common feature of most homobasidiomycetes, including ectomycorrhizal fungi. Production of extracellular peroxidases may provide ectomycorrhizal fungi with access to nitrogen sequestered in complex polyphenolic sources. The ISME Journal (2009) 3, 1387-1395; doi: 10.1038/ismej.2009.77; published online 2 July 2009

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