CLEC-2 is a phagocytic activation receptor expressed on murine peripheral blood neutrophils

Ann M Kerrigan; Kevin M Dennehy; Diego Mourão-Sá; Inês Faro-Trindade; Janet A Willment; Philip R Taylor; Johannes A Eble; Caetano Reis e Sousa; Gordon D Brown

doi:10.4049/jimmunol.0802808

CLEC-2 is a phagocytic activation receptor expressed on murine peripheral blood neutrophils

Ann M Kerrigan, Kevin M Dennehy, Diego Mourão-Sá, Inês Faro-Trindade, Janet A Willment, Philip R Taylor, Johannes A Eble, Caetano Reis e Sousa, Gordon D Brown

Applied Medicine

Research output: Contribution to journal › Article › peer-review

104 Citations (Scopus)

Abstract

CLEC-2 is a member of the "dectin-1 cluster" of C-type lectin-like receptors and was originally thought to be restricted to platelets. In this study, we demonstrate that murine CLEC-2 is also expressed by peripheral blood neutrophils, but only weakly by bone marrow or elicited inflammatory neutrophils. On circulating neutrophils, CLEC-2 can mediate phagocytosis of Ab-coated beads and the production of proinflammatory cytokines, including TNF-alpha, in response to the CLEC-2 ligand, rhodocytin. CLEC-2 possesses a tyrosine-based cytoplasmic motif similar to that of dectin-1, and we show using chimeric analyses that the activities of this receptor are dependent on this tyrosine. Like dectin-1, CLEC-2 can recruit the signaling kinase Syk in myeloid cells, however, stimulation of this pathway does not induce the respiratory burst. These data therefore demonstrate that CLEC-2 expression is not restricted to platelets and that it functions as an activation receptor on neutrophils.

Original language	English
Pages (from-to)	4150-4157
Number of pages	8
Journal	The Journal of Immunology
Volume	182
Issue number	7
DOIs	https://doi.org/10.4049/jimmunol.0802808
Publication status	Published - 1 Apr 2009

Keywords

animals
blotting, western
enzyme-linked immunosorbent assay
flow cytometry
gene expression
gene expression regulation
immunoprecipitation
intracellular signaling peptides and proteins
lectins, C-type
mice
neutrophils
phagocytosis
protein-tyrosine kinases
respiratory burst
tumor necrosis factor-alpha

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title = "CLEC-2 is a phagocytic activation receptor expressed on murine peripheral blood neutrophils",

abstract = "CLEC-2 is a member of the {"}dectin-1 cluster{"} of C-type lectin-like receptors and was originally thought to be restricted to platelets. In this study, we demonstrate that murine CLEC-2 is also expressed by peripheral blood neutrophils, but only weakly by bone marrow or elicited inflammatory neutrophils. On circulating neutrophils, CLEC-2 can mediate phagocytosis of Ab-coated beads and the production of proinflammatory cytokines, including TNF-alpha, in response to the CLEC-2 ligand, rhodocytin. CLEC-2 possesses a tyrosine-based cytoplasmic motif similar to that of dectin-1, and we show using chimeric analyses that the activities of this receptor are dependent on this tyrosine. Like dectin-1, CLEC-2 can recruit the signaling kinase Syk in myeloid cells, however, stimulation of this pathway does not induce the respiratory burst. These data therefore demonstrate that CLEC-2 expression is not restricted to platelets and that it functions as an activation receptor on neutrophils.",

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T1 - CLEC-2 is a phagocytic activation receptor expressed on murine peripheral blood neutrophils

AU - Kerrigan, Ann M

AU - Dennehy, Kevin M

AU - Mourão-Sá, Diego

AU - Faro-Trindade, Inês

AU - Willment, Janet A

AU - Taylor, Philip R

AU - Eble, Johannes A

AU - Reis e Sousa, Caetano

AU - Brown, Gordon D

PY - 2009/4/1

Y1 - 2009/4/1

N2 - CLEC-2 is a member of the "dectin-1 cluster" of C-type lectin-like receptors and was originally thought to be restricted to platelets. In this study, we demonstrate that murine CLEC-2 is also expressed by peripheral blood neutrophils, but only weakly by bone marrow or elicited inflammatory neutrophils. On circulating neutrophils, CLEC-2 can mediate phagocytosis of Ab-coated beads and the production of proinflammatory cytokines, including TNF-alpha, in response to the CLEC-2 ligand, rhodocytin. CLEC-2 possesses a tyrosine-based cytoplasmic motif similar to that of dectin-1, and we show using chimeric analyses that the activities of this receptor are dependent on this tyrosine. Like dectin-1, CLEC-2 can recruit the signaling kinase Syk in myeloid cells, however, stimulation of this pathway does not induce the respiratory burst. These data therefore demonstrate that CLEC-2 expression is not restricted to platelets and that it functions as an activation receptor on neutrophils.

AB - CLEC-2 is a member of the "dectin-1 cluster" of C-type lectin-like receptors and was originally thought to be restricted to platelets. In this study, we demonstrate that murine CLEC-2 is also expressed by peripheral blood neutrophils, but only weakly by bone marrow or elicited inflammatory neutrophils. On circulating neutrophils, CLEC-2 can mediate phagocytosis of Ab-coated beads and the production of proinflammatory cytokines, including TNF-alpha, in response to the CLEC-2 ligand, rhodocytin. CLEC-2 possesses a tyrosine-based cytoplasmic motif similar to that of dectin-1, and we show using chimeric analyses that the activities of this receptor are dependent on this tyrosine. Like dectin-1, CLEC-2 can recruit the signaling kinase Syk in myeloid cells, however, stimulation of this pathway does not induce the respiratory burst. These data therefore demonstrate that CLEC-2 expression is not restricted to platelets and that it functions as an activation receptor on neutrophils.

KW - animals

KW - blotting, western

KW - enzyme-linked immunosorbent assay

KW - flow cytometry

KW - gene expression

KW - gene expression regulation

KW - immunoprecipitation

KW - intracellular signaling peptides and proteins

KW - lectins, C-type

KW - mice

KW - neutrophils

KW - phagocytosis

KW - protein-tyrosine kinases

KW - respiratory burst

KW - tumor necrosis factor-alpha

U2 - 10.4049/jimmunol.0802808

DO - 10.4049/jimmunol.0802808

M3 - Article

C2 - 19299712

SN - 0022-1767

VL - 182

SP - 4150

EP - 4157

JO - The Journal of Immunology

JF - The Journal of Immunology

IS - 7

ER -

CLEC-2 is a phagocytic activation receptor expressed on murine peripheral blood neutrophils

Abstract

Keywords

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