CLEC9A is a novel activation C-type lectin-like receptor expressed on BDCA3+ dendritic cells and a subset of monocytes

Cristal Huysamen, Janet A. Willment, Kevin M. Dennehy, Gordon D. Brown

Research output: Contribution to journalArticle

202 Citations (Scopus)

Abstract

We describe here the first characterization of CLEC9A, a group V C-type lectin-like receptor located in the "Dectin-1 cluster" of related receptors, which are encoded within the natural killer (NK)-gene complex. Expression of human CLEC9A is highly restricted in peripheral blood, being detected only on BDCA3(+) dendritic cells and on a small subset of CD14(+)CD16(-) monocytes. CLEC9A is expressed at the cell surface as a glycosylated dimer and can mediate endocytosis, but not phagocytosis. CLEC9A possesses a cytoplasmic immunoreceptor tyrosine-based activation-like motif that can recruit Syk kinase, and we demonstrate, using receptor chimeras, that this receptor can induce proinflammatory cytokine production. These data indicate that CLEC9A functions as an activation receptor.
Original languageEnglish
Pages (from-to)16693-16701
Number of pages9
JournalThe Journal of Biological Chemistry
Volume283
Issue number24
Early online date11 Apr 2008
DOIs
Publication statusPublished - 13 Jun 2008

Keywords

  • amino acid motifs
  • amino acid sequence
  • animals
  • antigens, CD14
  • base sequence
  • dendritic cells
  • endocytosis
  • humans
  • intracellular signaling peptides and proteins
  • lectins, C-type
  • mice
  • models, biological
  • molecular sequence data
  • monocytes
  • protein-tyrosine kinases
  • receptors, IgG
  • receptors, mitogen

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