Clenbuterol increases the expression of myogenin but not myoD in immobilized rat muscles

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Abstract

Immobilization of one hindlimb of young rats in plantar flexion for 3 days led to changes in the plantaris muscles. These comprised a loss of muscle mass and a reduction in protein and RNA content, but no change in the transcript levels of the myogenic regulatory factors myogenin and myoD. Dietary administration of the beta-adrenoceptor agonist clenbuterol (2 mg/kg diet), which has been shown to ameliorate muscle wasting in a wide range of atrophic conditions, also limited muscle wasting in terms of weight, protein, and RNA in the immobilized plantaris muscles. In addition, drug treatment in immobilized plantaris muscles was associated with a marked increase in the steady-state levels of mRNA for myogenin (similar to 360% increase over control) but not myoD. These data provide the first evidence for independent changes in these two myogenic regulatory factors in immobilized muscle and suggest that the action of clenbuterol on these factors may depend on the mechanistic basis for the atrophic response.

Original languageEnglish
Pages (from-to)E941-E944
Number of pages4
JournalAmerican Journal of Physiology: Endocrinology and Metabolism
Volume272
Publication statusPublished - 1997

Keywords

  • disuse
  • myogenic regulatory factors
  • atrophy
  • gene expression
  • growth
  • SKELETAL-MUSCLE
  • AGONIST CLENBUTEROL
  • ATROPHY
  • STRESS

Cite this

@article{5e0bb7eef14c47cea95a33d4dea466e6,
title = "Clenbuterol increases the expression of myogenin but not myoD in immobilized rat muscles",
abstract = "Immobilization of one hindlimb of young rats in plantar flexion for 3 days led to changes in the plantaris muscles. These comprised a loss of muscle mass and a reduction in protein and RNA content, but no change in the transcript levels of the myogenic regulatory factors myogenin and myoD. Dietary administration of the beta-adrenoceptor agonist clenbuterol (2 mg/kg diet), which has been shown to ameliorate muscle wasting in a wide range of atrophic conditions, also limited muscle wasting in terms of weight, protein, and RNA in the immobilized plantaris muscles. In addition, drug treatment in immobilized plantaris muscles was associated with a marked increase in the steady-state levels of mRNA for myogenin (similar to 360{\%} increase over control) but not myoD. These data provide the first evidence for independent changes in these two myogenic regulatory factors in immobilized muscle and suggest that the action of clenbuterol on these factors may depend on the mechanistic basis for the atrophic response.",
keywords = "disuse, myogenic regulatory factors, atrophy, gene expression, growth, SKELETAL-MUSCLE, AGONIST CLENBUTEROL, ATROPHY, STRESS",
author = "Delday, {Margaret Inkster} and Maltin, {C A}",
year = "1997",
language = "English",
volume = "272",
pages = "E941--E944",
journal = "American Journal of Physiology: Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",

}

TY - JOUR

T1 - Clenbuterol increases the expression of myogenin but not myoD in immobilized rat muscles

AU - Delday, Margaret Inkster

AU - Maltin, C A

PY - 1997

Y1 - 1997

N2 - Immobilization of one hindlimb of young rats in plantar flexion for 3 days led to changes in the plantaris muscles. These comprised a loss of muscle mass and a reduction in protein and RNA content, but no change in the transcript levels of the myogenic regulatory factors myogenin and myoD. Dietary administration of the beta-adrenoceptor agonist clenbuterol (2 mg/kg diet), which has been shown to ameliorate muscle wasting in a wide range of atrophic conditions, also limited muscle wasting in terms of weight, protein, and RNA in the immobilized plantaris muscles. In addition, drug treatment in immobilized plantaris muscles was associated with a marked increase in the steady-state levels of mRNA for myogenin (similar to 360% increase over control) but not myoD. These data provide the first evidence for independent changes in these two myogenic regulatory factors in immobilized muscle and suggest that the action of clenbuterol on these factors may depend on the mechanistic basis for the atrophic response.

AB - Immobilization of one hindlimb of young rats in plantar flexion for 3 days led to changes in the plantaris muscles. These comprised a loss of muscle mass and a reduction in protein and RNA content, but no change in the transcript levels of the myogenic regulatory factors myogenin and myoD. Dietary administration of the beta-adrenoceptor agonist clenbuterol (2 mg/kg diet), which has been shown to ameliorate muscle wasting in a wide range of atrophic conditions, also limited muscle wasting in terms of weight, protein, and RNA in the immobilized plantaris muscles. In addition, drug treatment in immobilized plantaris muscles was associated with a marked increase in the steady-state levels of mRNA for myogenin (similar to 360% increase over control) but not myoD. These data provide the first evidence for independent changes in these two myogenic regulatory factors in immobilized muscle and suggest that the action of clenbuterol on these factors may depend on the mechanistic basis for the atrophic response.

KW - disuse

KW - myogenic regulatory factors

KW - atrophy

KW - gene expression

KW - growth

KW - SKELETAL-MUSCLE

KW - AGONIST CLENBUTEROL

KW - ATROPHY

KW - STRESS

M3 - Article

VL - 272

SP - E941-E944

JO - American Journal of Physiology: Endocrinology and Metabolism

JF - American Journal of Physiology: Endocrinology and Metabolism

SN - 0193-1849

ER -