Clenbuterol Mimics Effects of Innervation on Myogenic Regulatory Factor Expression

C A Maltin, Margaret Inkster Delday, Gillian Patricia Campbell, J E Hesketh

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18 Citations (Scopus)

Abstract

The amelioration of denervation atrophy by the beta-adrenoceptor agonist clenbuterol has led to the suggestion that the drug mimics or stimulates production of neurotrophic factors. Neurotrophic factors have profound effects on muscle growth, but the precise mechanisms through which this influence is exerted are unknown. The expression of myoD and myogenin, proteins that in turn regulate the transcription of tissue-specific genes during skeletal muscle differentiation, is controlled by innervation. In muscle undergoing denervation-induced atrophy, myoD and myogenin mRNAs increase. However, this is only partially reversed by electrical activity, thus implicating neurotrophic factors in regulation of these genes. Here we demonstrate that clenbuterol represses myoD and myogenin expression and decreases the levels of acetylcholine receptors in denervated muscles. The data provide the first evidence that the action of clenbuterol is directed through the neural axis.

Original languageEnglish
Pages (from-to)E176-E178
Number of pages3
JournalAMERICAN JOURNAL OF PHYSIOLOGY
Volume265
Issue number1
Publication statusPublished - Jul 1993

Keywords

  • MUSCLE
  • GROWTH
  • RAT
  • MYOD
  • MYOGENIN
  • BETA-AGONIST
  • ACETYLCHOLINE RECEPTOR
  • DENERVATION
  • ACETYLCHOLINE-RECEPTOR GENES
  • SKELETAL-MUSCLE
  • AGONIST CLENBUTEROL
  • PROTEIN-METABOLISM
  • RAT MUSCLE
  • muscle
  • growth
  • rat
  • myod
  • myogenin
  • beta-agonist
  • acetylcholine receptor
  • denervation

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