Clenbuterol Mimics Effects of Innervation on Myogenic Regulatory Factor Expression

C A Maltin, Margaret Inkster Delday, Gillian Patricia Campbell, J E Hesketh

Research output: Contribution to journalComment/debate

17 Citations (Scopus)

Abstract

The amelioration of denervation atrophy by the beta-adrenoceptor agonist clenbuterol has led to the suggestion that the drug mimics or stimulates production of neurotrophic factors. Neurotrophic factors have profound effects on muscle growth, but the precise mechanisms through which this influence is exerted are unknown. The expression of myoD and myogenin, proteins that in turn regulate the transcription of tissue-specific genes during skeletal muscle differentiation, is controlled by innervation. In muscle undergoing denervation-induced atrophy, myoD and myogenin mRNAs increase. However, this is only partially reversed by electrical activity, thus implicating neurotrophic factors in regulation of these genes. Here we demonstrate that clenbuterol represses myoD and myogenin expression and decreases the levels of acetylcholine receptors in denervated muscles. The data provide the first evidence that the action of clenbuterol is directed through the neural axis.

Original languageEnglish
Pages (from-to)E176-E178
Number of pages3
JournalAMERICAN JOURNAL OF PHYSIOLOGY
Volume265
Issue number1
Publication statusPublished - Jul 1993

Keywords

  • MUSCLE
  • GROWTH
  • RAT
  • MYOD
  • MYOGENIN
  • BETA-AGONIST
  • ACETYLCHOLINE RECEPTOR
  • DENERVATION
  • ACETYLCHOLINE-RECEPTOR GENES
  • SKELETAL-MUSCLE
  • AGONIST CLENBUTEROL
  • PROTEIN-METABOLISM
  • RAT MUSCLE
  • muscle
  • growth
  • rat
  • myod
  • myogenin
  • beta-agonist
  • acetylcholine receptor
  • denervation

Cite this

Clenbuterol Mimics Effects of Innervation on Myogenic Regulatory Factor Expression. / Maltin, C A ; Delday, Margaret Inkster; Campbell, Gillian Patricia; Hesketh, J E .

In: AMERICAN JOURNAL OF PHYSIOLOGY, Vol. 265, No. 1, 07.1993, p. E176-E178.

Research output: Contribution to journalComment/debate

Maltin, C A ; Delday, Margaret Inkster ; Campbell, Gillian Patricia ; Hesketh, J E . / Clenbuterol Mimics Effects of Innervation on Myogenic Regulatory Factor Expression. In: AMERICAN JOURNAL OF PHYSIOLOGY. 1993 ; Vol. 265, No. 1. pp. E176-E178.
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abstract = "The amelioration of denervation atrophy by the beta-adrenoceptor agonist clenbuterol has led to the suggestion that the drug mimics or stimulates production of neurotrophic factors. Neurotrophic factors have profound effects on muscle growth, but the precise mechanisms through which this influence is exerted are unknown. The expression of myoD and myogenin, proteins that in turn regulate the transcription of tissue-specific genes during skeletal muscle differentiation, is controlled by innervation. In muscle undergoing denervation-induced atrophy, myoD and myogenin mRNAs increase. However, this is only partially reversed by electrical activity, thus implicating neurotrophic factors in regulation of these genes. Here we demonstrate that clenbuterol represses myoD and myogenin expression and decreases the levels of acetylcholine receptors in denervated muscles. The data provide the first evidence that the action of clenbuterol is directed through the neural axis.",
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AU - Maltin, C A

AU - Delday, Margaret Inkster

AU - Campbell, Gillian Patricia

AU - Hesketh, J E

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N2 - The amelioration of denervation atrophy by the beta-adrenoceptor agonist clenbuterol has led to the suggestion that the drug mimics or stimulates production of neurotrophic factors. Neurotrophic factors have profound effects on muscle growth, but the precise mechanisms through which this influence is exerted are unknown. The expression of myoD and myogenin, proteins that in turn regulate the transcription of tissue-specific genes during skeletal muscle differentiation, is controlled by innervation. In muscle undergoing denervation-induced atrophy, myoD and myogenin mRNAs increase. However, this is only partially reversed by electrical activity, thus implicating neurotrophic factors in regulation of these genes. Here we demonstrate that clenbuterol represses myoD and myogenin expression and decreases the levels of acetylcholine receptors in denervated muscles. The data provide the first evidence that the action of clenbuterol is directed through the neural axis.

AB - The amelioration of denervation atrophy by the beta-adrenoceptor agonist clenbuterol has led to the suggestion that the drug mimics or stimulates production of neurotrophic factors. Neurotrophic factors have profound effects on muscle growth, but the precise mechanisms through which this influence is exerted are unknown. The expression of myoD and myogenin, proteins that in turn regulate the transcription of tissue-specific genes during skeletal muscle differentiation, is controlled by innervation. In muscle undergoing denervation-induced atrophy, myoD and myogenin mRNAs increase. However, this is only partially reversed by electrical activity, thus implicating neurotrophic factors in regulation of these genes. Here we demonstrate that clenbuterol represses myoD and myogenin expression and decreases the levels of acetylcholine receptors in denervated muscles. The data provide the first evidence that the action of clenbuterol is directed through the neural axis.

KW - MUSCLE

KW - GROWTH

KW - RAT

KW - MYOD

KW - MYOGENIN

KW - BETA-AGONIST

KW - ACETYLCHOLINE RECEPTOR

KW - DENERVATION

KW - ACETYLCHOLINE-RECEPTOR GENES

KW - SKELETAL-MUSCLE

KW - AGONIST CLENBUTEROL

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KW - RAT MUSCLE

KW - muscle

KW - growth

KW - rat

KW - myod

KW - myogenin

KW - beta-agonist

KW - acetylcholine receptor

KW - denervation

M3 - Comment/debate

VL - 265

SP - E176-E178

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0002-9513

IS - 1

ER -