Clonal regulatory T cells specific for a red blood cell autoantigen in human autoimmune hemolytic anemia

Frank J. Ward, Andrew M. Hall, Lindsay S. Cairns, Arabella S. Leggat, Stanislaw J. Urbaniak, Mark A. Vickers, Robert N. Barker

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Regulatory T (Tr) cells have the potential to treat immune-mediated disease, but cloning such cells for study from patients with autoimmune disease has proven difficult. Here, we describe autoantigen-specific, interleukin-10 (IL-10)-secreting Tr cell clones recovered ex vivo from a patient with autoimmune hemolytic anemia (AIHA) and characterize their phenotype, origin, and regulatory function. These IL-10+ Tr cells recognized a peptide, 72H-86L, derived from the Rh red blood cell autoantigen and shared phenotypic characteristics with both natural and inducible Tr cells. The clones also expressed different Tr markers depending on activation state: high levels of CD25 and LAG-3 when expanding nonspecifically, but FoxP3 after activation by the autoantigen they recognize. Despite a discrete Tr phenotype, these cells stably expressed the T helper 1 (Th1) signature transcription factor T-bet, suggesting they derive from Th1 T cells. Finally, the contribution of CTLA-4 in activating these IL-10+ Tr cells was confirmed by analyzing responses to transgenic B7.1-like molecules that preferentially bind either CD28 or CTLA-4. Overall, these Tr cells have a functional phenotype different from those described in previous studies of human Tr populations, which have not taken account of antigen specificity, and understanding their properties will enable them to be exploited therapeutically in AIHA.
Original languageEnglish
Pages (from-to)680-687
Number of pages8
JournalBlood
Volume111
Issue number2
Early online date30 Aug 2007
DOIs
Publication statusPublished - 15 Jan 2008

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Autoimmune Hemolytic Anemia
T-cells
Autoantigens
Regulatory T-Lymphocytes
Interleukin-10
Blood
Erythrocytes
Cells
Clone cells
Chemical activation
Cloning
Phenotype
Clone Cells
Antigens
Peptides
Th1 Cells
Molecules
Immune System Diseases
Autoimmune Diseases
Organism Cloning

Keywords

  • aged
  • anemia, hemolytic, autoimmune
  • antigens, CD
  • antigens, CD80
  • antigens, differentiation
  • autoantigens
  • cell line
  • female
  • forkhead transcription factors
  • humans
  • interleukin-10
  • interleukin-2 receptor alpha subunit
  • lymphocyte activation
  • lymphocyte transfusion
  • peptides
  • rh-hr blood-group system
  • t-box domain proteins
  • t-lymphocytes, regulatory

Cite this

Clonal regulatory T cells specific for a red blood cell autoantigen in human autoimmune hemolytic anemia. / Ward, Frank J.; Hall, Andrew M.; Cairns, Lindsay S.; Leggat, Arabella S.; Urbaniak, Stanislaw J.; Vickers, Mark A.; Barker, Robert N.

In: Blood, Vol. 111, No. 2, 15.01.2008, p. 680-687.

Research output: Contribution to journalArticle

Ward, Frank J. ; Hall, Andrew M. ; Cairns, Lindsay S. ; Leggat, Arabella S. ; Urbaniak, Stanislaw J. ; Vickers, Mark A. ; Barker, Robert N. / Clonal regulatory T cells specific for a red blood cell autoantigen in human autoimmune hemolytic anemia. In: Blood. 2008 ; Vol. 111, No. 2. pp. 680-687.
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