Cloning and characterisation of a putative ST2L homologue from Atlantic salmon (Salmo salar)

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Abstract

The ST2L receptor is a member of the interleukin-1 (IL-1) receptor family and has previously been cloned from human, mouse, rat and chicken. This orphan receptor has no known physiological role but has been implicated in T helper cell type 2 effector function. We describe in this report the cloning and characterisation of a cDNA encoding a homologue of ST2L in Atlantic salmon (Salmo salar). The salmon ST2L cDNA is 2364 bp in length and has an open reading frame encoding a polypeptide of 582 amino acids. Similar to other members of the IL-1 receptor (IL-1R) family, the predicted protein has a potential signal peptide, extracellular immunoglobulin-like domains, a short transmembrane region and a characteristic cytoplasmic Toll-IL-1R domain. The predicted protein shows 33% identity and 44% similarity to the chicken ST2L homologue. Phylogenetic analyses cluster the putative salmon ST2L with the chicken and the mammalian ST2L homologues, away from the other members of the IL-1R family. Salmon ST2L is constitutively expressed in brain, white and red blood cells, head kidney, liver, gills and muscle, with highest level of expression in spleen. In vivo stimulation of salmon with lipopolysaccaride does not appear to have a significant effect on expression of the ST2L homologue. (C) 2003 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)211-224
Number of pages13
JournalFish & Shellfish Immunology
Volume15
DOIs
Publication statusPublished - 2003

Keywords

  • ST2L
  • Salmo salar
  • IL-1R family
  • TIR domain
  • Ig domain
  • HELPER T-CELLS
  • INTERLEUKIN-1 RECEPTOR
  • PROMOTER USAGE
  • IL-1 RECEPTOR
  • VARIANT FORM
  • GENE-PRODUCT
  • EXPRESSION
  • CDNA
  • SEQUENCE
  • PROTEIN

Cite this

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title = "Cloning and characterisation of a putative ST2L homologue from Atlantic salmon (Salmo salar)",
abstract = "The ST2L receptor is a member of the interleukin-1 (IL-1) receptor family and has previously been cloned from human, mouse, rat and chicken. This orphan receptor has no known physiological role but has been implicated in T helper cell type 2 effector function. We describe in this report the cloning and characterisation of a cDNA encoding a homologue of ST2L in Atlantic salmon (Salmo salar). The salmon ST2L cDNA is 2364 bp in length and has an open reading frame encoding a polypeptide of 582 amino acids. Similar to other members of the IL-1 receptor (IL-1R) family, the predicted protein has a potential signal peptide, extracellular immunoglobulin-like domains, a short transmembrane region and a characteristic cytoplasmic Toll-IL-1R domain. The predicted protein shows 33{\%} identity and 44{\%} similarity to the chicken ST2L homologue. Phylogenetic analyses cluster the putative salmon ST2L with the chicken and the mammalian ST2L homologues, away from the other members of the IL-1R family. Salmon ST2L is constitutively expressed in brain, white and red blood cells, head kidney, liver, gills and muscle, with highest level of expression in spleen. In vivo stimulation of salmon with lipopolysaccaride does not appear to have a significant effect on expression of the ST2L homologue. (C) 2003 Elsevier Ltd. All rights reserved.",
keywords = "ST2L, Salmo salar, IL-1R family, TIR domain, Ig domain, HELPER T-CELLS, INTERLEUKIN-1 RECEPTOR, PROMOTER USAGE, IL-1 RECEPTOR, VARIANT FORM, GENE-PRODUCT, EXPRESSION, CDNA, SEQUENCE, PROTEIN",
author = "Secombes, {Christopher John}",
year = "2003",
doi = "10.1016/S1050-4648(02)00162-6",
language = "English",
volume = "15",
pages = "211--224",
journal = "Fish & Shellfish Immunology",
issn = "1050-4648",
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TY - JOUR

T1 - Cloning and characterisation of a putative ST2L homologue from Atlantic salmon (Salmo salar)

AU - Secombes, Christopher John

PY - 2003

Y1 - 2003

N2 - The ST2L receptor is a member of the interleukin-1 (IL-1) receptor family and has previously been cloned from human, mouse, rat and chicken. This orphan receptor has no known physiological role but has been implicated in T helper cell type 2 effector function. We describe in this report the cloning and characterisation of a cDNA encoding a homologue of ST2L in Atlantic salmon (Salmo salar). The salmon ST2L cDNA is 2364 bp in length and has an open reading frame encoding a polypeptide of 582 amino acids. Similar to other members of the IL-1 receptor (IL-1R) family, the predicted protein has a potential signal peptide, extracellular immunoglobulin-like domains, a short transmembrane region and a characteristic cytoplasmic Toll-IL-1R domain. The predicted protein shows 33% identity and 44% similarity to the chicken ST2L homologue. Phylogenetic analyses cluster the putative salmon ST2L with the chicken and the mammalian ST2L homologues, away from the other members of the IL-1R family. Salmon ST2L is constitutively expressed in brain, white and red blood cells, head kidney, liver, gills and muscle, with highest level of expression in spleen. In vivo stimulation of salmon with lipopolysaccaride does not appear to have a significant effect on expression of the ST2L homologue. (C) 2003 Elsevier Ltd. All rights reserved.

AB - The ST2L receptor is a member of the interleukin-1 (IL-1) receptor family and has previously been cloned from human, mouse, rat and chicken. This orphan receptor has no known physiological role but has been implicated in T helper cell type 2 effector function. We describe in this report the cloning and characterisation of a cDNA encoding a homologue of ST2L in Atlantic salmon (Salmo salar). The salmon ST2L cDNA is 2364 bp in length and has an open reading frame encoding a polypeptide of 582 amino acids. Similar to other members of the IL-1 receptor (IL-1R) family, the predicted protein has a potential signal peptide, extracellular immunoglobulin-like domains, a short transmembrane region and a characteristic cytoplasmic Toll-IL-1R domain. The predicted protein shows 33% identity and 44% similarity to the chicken ST2L homologue. Phylogenetic analyses cluster the putative salmon ST2L with the chicken and the mammalian ST2L homologues, away from the other members of the IL-1R family. Salmon ST2L is constitutively expressed in brain, white and red blood cells, head kidney, liver, gills and muscle, with highest level of expression in spleen. In vivo stimulation of salmon with lipopolysaccaride does not appear to have a significant effect on expression of the ST2L homologue. (C) 2003 Elsevier Ltd. All rights reserved.

KW - ST2L

KW - Salmo salar

KW - IL-1R family

KW - TIR domain

KW - Ig domain

KW - HELPER T-CELLS

KW - INTERLEUKIN-1 RECEPTOR

KW - PROMOTER USAGE

KW - IL-1 RECEPTOR

KW - VARIANT FORM

KW - GENE-PRODUCT

KW - EXPRESSION

KW - CDNA

KW - SEQUENCE

KW - PROTEIN

U2 - 10.1016/S1050-4648(02)00162-6

DO - 10.1016/S1050-4648(02)00162-6

M3 - Article

VL - 15

SP - 211

EP - 224

JO - Fish & Shellfish Immunology

JF - Fish & Shellfish Immunology

SN - 1050-4648

ER -