Cloning and characterization of the Rainbow trout (Oncorhynchus mykiss) type II interleukin-1 receptor cDNA

A Sangrador-Vegas, Samuel Allen Moore Martin, P G O'Dea, T J Smith

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Abstract

A homologue of mammalian type II interleukin-1 receptor (IL-1RII) was isolated from a rainbow trout cDNA library by differential hybridization using a suppression subtractive hybridization generated probe enriched far sequences upregulated after immune stimulation. The trout cDNA has an ORF encoding 441 amino acids, and represents the first piscine IL-1 receptor described. The predicted amino-acid sequence has 29 and 26% identity with human and mouse IL-1RII, respectively. The trout IL-1 receptor has a domain organization similar to that of mammalian type II receptor, with a short cytoplasmic tail of 24 amino acids. These results suggest that type II receptor is also present in lower vertebrates, and therefore the duplication of an ancestral gene that generated type I and type II IL-1 receptors occurred prior to the time mammals emerged.

Original languageEnglish
Pages (from-to)7031-7037
Number of pages7
JournalEuropean Journal of Biochemistry
Volume267
Issue number24
Publication statusPublished - Dec 2000

Keywords

  • cDNA sequence
  • differential hybridization
  • Oncorhynchus mykiss
  • suppression subtractive hybridization
  • type II interleukin-1 receptor
  • IL-1 receptor
  • molecular-cloning
  • messenger-RNA
  • decoy target
  • expression
  • chemokine
  • affinity
  • gene
  • identification

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