Cloning and expression analysis of three striped trumpeter (Latris lineata) pro-inflammatory cytokines, TNF-α, IL-1β and IL-8, in response to infection by the ectoparasitic, Chondracanthus goldsmidi

J M Covello, S Bird, R N Morrison, S C Battaglene, C J Secombes, B F Nowak

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

This study reports the cloning and sequencing of three striped trumpeter (Latris lineata Forster) pro-inflammatory cytokines, TNF-alpha, IL-1 beta and IL-8, as well as their differential expression in response to an infection by the ectoparasite Chondracanthus goldsmidi. The striped trumpeter TNF-a transcript consisted of 1093 bp, including a 759 bp ORF which translated into a 253 aa transmembrane peptide. The sequence contained a TACE cut site, that would produce a 167 aa soluble peptide containing the TNF ligand family signature. The IL-1 beta sequence consisted of 963 bp, including a 774 bp ORF which translated into a 258 aa protein. The protein lacked both a signal peptide and an ICE cleavage site, but did contain the IL-1 family signature. The sequence for the chemokine IL-8 contained 906 bp, with an ORF of 297 bp, which translated into a 99 aa protein. The protein lacked an ELR motif as is common with many teleost IL-8 sequences. The differential expression of the three cytokine genes in parasitized fish was investigated via quantitative real-time PCR. A significant up-regulation of all three pro-inflammatory cytokines was found in the gills, which were the site of parasite attachment. Examination of head kidney cells revealed a significant up-regulation of TNF-a, but not IL-1 beta or IL-8. Conversely, the spleen cells showed significant up-regulation of both IL-1 beta and IL-8, but not TNF-alpha. These findings allow for more detailed investigations of the striped trumpeter immune response. (C) 2009 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)773-786
Number of pages14
JournalFish & Shellfish Immunology
Volume26
Issue number5
DOIs
Publication statusPublished - May 2009

Fingerprint

Latris lineata
Cloning
interleukin-8
interleukin-1
Interleukin-8
Interleukin-1
molecular cloning
cytokines
Interleukin-1beta
peptide
Cytokines
protein
open reading frames
infection
tumor necrosis factor-alpha
Proteins
proteins
Tumor Necrosis Factor-alpha
ectoparasite
peptides

Keywords

  • inflammatory response
  • TNF-alpha
  • IL-1 beta
  • IL-8
  • ectoparasite

Cite this

Cloning and expression analysis of three striped trumpeter (Latris lineata) pro-inflammatory cytokines, TNF-α, IL-1β and IL-8, in response to infection by the ectoparasitic, Chondracanthus goldsmidi. / Covello, J M; Bird, S; Morrison, R N; Battaglene, S C; Secombes, C J; Nowak, B F.

In: Fish & Shellfish Immunology, Vol. 26, No. 5, 05.2009, p. 773-786.

Research output: Contribution to journalArticle

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