Cloning and expression of single chain antibody fragments in Escherichia coli and Pichia pastoris

P M Cupit, J A Whyte, A J Porter, M J Browne, S D Holmes, W J Harris, C Cunningham

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The potential of recombinant antibody fragments is likely to be fulfilled only if they can be produced routinely at high concentrations. We have compared the ability of Escherichia coli and Pichia pastoris to produce functional recombinant single chain antibody (scAb) fragments. Two scAb fragments were expressed, an antihuman type V acid phosphatase (TRAP) and an anti-Pseudomonas aeruginosa lipoprotein I. We report here that, while expression from P. pastoris resulted in a significantly increased level of expression of the anti-TRAP scAb compared to E. coli, neither fragment was able to bind its target antigen as well as the bacterial product.

Original languageEnglish
Pages (from-to)273-277
Number of pages5
JournalLetters in Applied Microbiology
Volume29
Publication statusPublished - 1999

Keywords

  • HIGH-LEVEL SECRETION
  • METHYLOTROPHIC YEAST
  • GENERATION
  • SYSTEM

Cite this

Cupit, P. M., Whyte, J. A., Porter, A. J., Browne, M. J., Holmes, S. D., Harris, W. J., & Cunningham, C. (1999). Cloning and expression of single chain antibody fragments in Escherichia coli and Pichia pastoris. Letters in Applied Microbiology, 29, 273-277.

Cloning and expression of single chain antibody fragments in Escherichia coli and Pichia pastoris. / Cupit, P M ; Whyte, J A ; Porter, A J ; Browne, M J ; Holmes, S D ; Harris, W J ; Cunningham, C .

In: Letters in Applied Microbiology, Vol. 29, 1999, p. 273-277.

Research output: Contribution to journalArticle

Cupit, PM, Whyte, JA, Porter, AJ, Browne, MJ, Holmes, SD, Harris, WJ & Cunningham, C 1999, 'Cloning and expression of single chain antibody fragments in Escherichia coli and Pichia pastoris', Letters in Applied Microbiology, vol. 29, pp. 273-277.
Cupit, P M ; Whyte, J A ; Porter, A J ; Browne, M J ; Holmes, S D ; Harris, W J ; Cunningham, C . / Cloning and expression of single chain antibody fragments in Escherichia coli and Pichia pastoris. In: Letters in Applied Microbiology. 1999 ; Vol. 29. pp. 273-277.
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AU - Holmes, S D

AU - Harris, W J

AU - Cunningham, C

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