Cloning and sequence analysis of rainbow trout LMP 2 cDNA and differential expression of the mRNA

J Davidson, T Smith, S A M Martin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A Rainbow trout Oncorhynchus mykiss (L.) clone encoding the low molecular weight proteasome LMP 2 has been cloned and sequenced from rainbow trout leukocyte cells that had been stimulated with the mitogen phytohemagglutinin (PHA). The cDNA was 1254 bp and had an open reading frame of 648 bp encoding 217 amino acids. The sequence has a high degree of identity with other published LMP 2 sequences varying from 84.4%;, identity with medaka (Oryzias latipes) to 61.4% identity with Xenopus laevis. Northern blot analysis showed the LMP 2 mRNA to be expressed in a wide range of tissues, with two transcripts in gill, spleen and anterior kidney RNA. Up regulation of the LMP 2 mRNA transcript was shown in cells that were maintained in primary culture following stimulation with the PHA mitogen for 24 h. A 37-fold increase in mRNA was observed between control and stimulated cells. (C) 1999 Academic Press.

Original languageEnglish
Pages (from-to)621-632
Number of pages12
JournalFish & Shellfish Immunology
Volume9
Issue number8
DOIs
Publication statusPublished - Nov 1999

Keywords

  • proteasome
  • LMP2
  • mRNA expression
  • rainbow trout
  • cDNA sequence
  • Oncorhynchus mykiss L.
  • MHC CLASS-I
  • ADAPTIVE IMMUNE-SYSTEM
  • INTERFERON-GAMMA
  • ANTIGEN PRESENTATION
  • PROTEASOME
  • GENES
  • LINKAGE
  • FISH
  • VERTEBRATES
  • DUPLICATION

Cite this

Cloning and sequence analysis of rainbow trout LMP 2 cDNA and differential expression of the mRNA. / Davidson, J ; Smith, T ; Martin, S A M .

In: Fish & Shellfish Immunology, Vol. 9, No. 8, 11.1999, p. 621-632.

Research output: Contribution to journalArticle

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abstract = "A Rainbow trout Oncorhynchus mykiss (L.) clone encoding the low molecular weight proteasome LMP 2 has been cloned and sequenced from rainbow trout leukocyte cells that had been stimulated with the mitogen phytohemagglutinin (PHA). The cDNA was 1254 bp and had an open reading frame of 648 bp encoding 217 amino acids. The sequence has a high degree of identity with other published LMP 2 sequences varying from 84.4{\%};, identity with medaka (Oryzias latipes) to 61.4{\%} identity with Xenopus laevis. Northern blot analysis showed the LMP 2 mRNA to be expressed in a wide range of tissues, with two transcripts in gill, spleen and anterior kidney RNA. Up regulation of the LMP 2 mRNA transcript was shown in cells that were maintained in primary culture following stimulation with the PHA mitogen for 24 h. A 37-fold increase in mRNA was observed between control and stimulated cells. (C) 1999 Academic Press.",
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N2 - A Rainbow trout Oncorhynchus mykiss (L.) clone encoding the low molecular weight proteasome LMP 2 has been cloned and sequenced from rainbow trout leukocyte cells that had been stimulated with the mitogen phytohemagglutinin (PHA). The cDNA was 1254 bp and had an open reading frame of 648 bp encoding 217 amino acids. The sequence has a high degree of identity with other published LMP 2 sequences varying from 84.4%;, identity with medaka (Oryzias latipes) to 61.4% identity with Xenopus laevis. Northern blot analysis showed the LMP 2 mRNA to be expressed in a wide range of tissues, with two transcripts in gill, spleen and anterior kidney RNA. Up regulation of the LMP 2 mRNA transcript was shown in cells that were maintained in primary culture following stimulation with the PHA mitogen for 24 h. A 37-fold increase in mRNA was observed between control and stimulated cells. (C) 1999 Academic Press.

AB - A Rainbow trout Oncorhynchus mykiss (L.) clone encoding the low molecular weight proteasome LMP 2 has been cloned and sequenced from rainbow trout leukocyte cells that had been stimulated with the mitogen phytohemagglutinin (PHA). The cDNA was 1254 bp and had an open reading frame of 648 bp encoding 217 amino acids. The sequence has a high degree of identity with other published LMP 2 sequences varying from 84.4%;, identity with medaka (Oryzias latipes) to 61.4% identity with Xenopus laevis. Northern blot analysis showed the LMP 2 mRNA to be expressed in a wide range of tissues, with two transcripts in gill, spleen and anterior kidney RNA. Up regulation of the LMP 2 mRNA transcript was shown in cells that were maintained in primary culture following stimulation with the PHA mitogen for 24 h. A 37-fold increase in mRNA was observed between control and stimulated cells. (C) 1999 Academic Press.

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KW - Oncorhynchus mykiss L.

KW - MHC CLASS-I

KW - ADAPTIVE IMMUNE-SYSTEM

KW - INTERFERON-GAMMA

KW - ANTIGEN PRESENTATION

KW - PROTEASOME

KW - GENES

KW - LINKAGE

KW - FISH

KW - VERTEBRATES

KW - DUPLICATION

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