Abstract
This report describes the cloning and characterisation of rainbow trout (Oncorhynchus mykiss) interleukin (IL)-22, and presents studies of the functional activity of its recombinant protein for the first time in a non-mammalian species. The predicted IL-22 coding region consists of 522 nucleotides which translates into a 173 amino acid protein, that contains an IL-10 family signature which is reasonably well conserved with other vertebrate IL-22 molecules. Expression analysis in tissues from healthy fish revealed a higher constitutive expression of IL-22 in mucosal tissues, suggesting a potentially important role in mucosal immunity. In vitro studies demonstrated that IL-22 expression was induced significantly by PHA and PMA in splenocyte primary cultures 4h post-stimulation. Expression was also induced in the spleen upon infection of fish with the Gram-negative bacterium Yersinia ruckeri, suggesting a potential role of IL-22 in vivo in defence against bacterial diseases. The Escherichia coli produced recombinant IL-22 enhanced the expression of a number of antimicrobial peptides, promoting host innate immunity against microbes and revealing a biological similarity with its mammalian counterpart.
Original language | English |
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Pages (from-to) | 62-73 |
Number of pages | 12 |
Journal | Cytokine |
Volume | 55 |
Issue number | 1 |
Early online date | 21 Apr 2011 |
DOIs | |
Publication status | Published - Jul 2011 |
Keywords
- 5' flanking region
- amino acid sequence
- animals
- antimicrobial cationic peptides
- base sequence
- binding sites
- cloning, molecular
- gene expression profiling
- gene expression regulation
- humans
- interleukins
- introns
- molecular sequence data
- Oncorhynchus mykiss
- phylogeny
- protein binding
- recombinant proteins
- sequence alignment
- sequence analysis, DNA
- sequence homology, amino acid
- spleen
- transcription factors
- Yersinia ruckeri
- interleukin-22
- mucosal expression
- recombinant IL-22
- antimicrobial peptides