Cloning, expression analysis and bioactivity studies of rainbow trout (Onchorhynchus mykiss) interleukin-22

Milena M Monte, Jun Zou, Tiehui Wang, Allison Carrington, Chris J Secombes

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

This report describes the cloning and characterisation of rainbow trout (Oncorhynchus mykiss) interleukin (IL)-22, and presents studies of the functional activity of its recombinant protein for the first time in a non-mammalian species. The predicted IL-22 coding region consists of 522 nucleotides which translates into a 173 amino acid protein, that contains an IL-10 family signature which is reasonably well conserved with other vertebrate IL-22 molecules. Expression analysis in tissues from healthy fish revealed a higher constitutive expression of IL-22 in mucosal tissues, suggesting a potentially important role in mucosal immunity. In vitro studies demonstrated that IL-22 expression was induced significantly by PHA and PMA in splenocyte primary cultures 4h post-stimulation. Expression was also induced in the spleen upon infection of fish with the Gram-negative bacterium Yersinia ruckeri, suggesting a potential role of IL-22 in vivo in defence against bacterial diseases. The Escherichia coli produced recombinant IL-22 enhanced the expression of a number of antimicrobial peptides, promoting host innate immunity against microbes and revealing a biological similarity with its mammalian counterpart.
Original languageEnglish
Pages (from-to)62-73
Number of pages12
JournalCytokine
Volume55
Issue number1
Early online date21 Apr 2011
DOIs
Publication statusPublished - Jul 2011

Fingerprint

Cloning
Oncorhynchus mykiss
Bioactivity
Organism Cloning
Fish
Yersinia ruckeri
Fishes
Mucosal Immunity
interleukin-22
Gram-Negative Bacteria
Recombinant Proteins
Innate Immunity
Interleukin-10
Escherichia coli
Vertebrates
Bacteria
Mucous Membrane
Spleen
Nucleotides
Tissue

Keywords

  • 5' flanking region
  • amino acid sequence
  • animals
  • antimicrobial cationic peptides
  • base sequence
  • binding sites
  • cloning, molecular
  • gene expression profiling
  • gene expression regulation
  • humans
  • interleukins
  • introns
  • molecular sequence data
  • Oncorhynchus mykiss
  • phylogeny
  • protein binding
  • recombinant proteins
  • sequence alignment
  • sequence analysis, DNA
  • sequence homology, amino acid
  • spleen
  • transcription factors
  • Yersinia ruckeri
  • interleukin-22
  • mucosal expression
  • recombinant IL-22
  • antimicrobial peptides

Cite this

Cloning, expression analysis and bioactivity studies of rainbow trout (Onchorhynchus mykiss) interleukin-22. / Monte, Milena M; Zou, Jun; Wang, Tiehui; Carrington, Allison; Secombes, Chris J.

In: Cytokine, Vol. 55, No. 1, 07.2011, p. 62-73.

Research output: Contribution to journalArticle

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