Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 62-73 |
| Number of pages | 12 |
| Journal | Cytokine |
| Volume | 55 |
| Issue number | 1 |
| Early online date | 21 Apr 2011 |
| DOIs | |
| Publication status | Published - Jul 2011 |
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Keywords
- 5' flanking region
- amino acid sequence
- animals
- antimicrobial cationic peptides
- base sequence
- binding sites
- cloning, molecular
- gene expression profiling
- gene expression regulation
- humans
- interleukins
- introns
- molecular sequence data
- Oncorhynchus mykiss
- phylogeny
- protein binding
- recombinant proteins
- sequence alignment
- sequence analysis, DNA
- sequence homology, amino acid
- spleen
- transcription factors
- Yersinia ruckeri
- interleukin-22
- mucosal expression
- recombinant IL-22
- antimicrobial peptides
Cite this
Cloning, expression analysis and bioactivity studies of rainbow trout (Onchorhynchus mykiss) interleukin-22. / Monte, Milena M; Zou, Jun; Wang, Tiehui; Carrington, Allison; Secombes, Chris J.
In: Cytokine, Vol. 55, No. 1, 07.2011, p. 62-73.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning, expression analysis and bioactivity studies of rainbow trout (Onchorhynchus mykiss) interleukin-22
AU - Monte, Milena M
AU - Zou, Jun
AU - Wang, Tiehui
AU - Carrington, Allison
AU - Secombes, Chris J
N1 - Copyright © 2011 Elsevier Ltd. All rights reserved.
PY - 2011/7
Y1 - 2011/7
N2 - This report describes the cloning and characterisation of rainbow trout (Oncorhynchus mykiss) interleukin (IL)-22, and presents studies of the functional activity of its recombinant protein for the first time in a non-mammalian species. The predicted IL-22 coding region consists of 522 nucleotides which translates into a 173 amino acid protein, that contains an IL-10 family signature which is reasonably well conserved with other vertebrate IL-22 molecules. Expression analysis in tissues from healthy fish revealed a higher constitutive expression of IL-22 in mucosal tissues, suggesting a potentially important role in mucosal immunity. In vitro studies demonstrated that IL-22 expression was induced significantly by PHA and PMA in splenocyte primary cultures 4h post-stimulation. Expression was also induced in the spleen upon infection of fish with the Gram-negative bacterium Yersinia ruckeri, suggesting a potential role of IL-22 in vivo in defence against bacterial diseases. The Escherichia coli produced recombinant IL-22 enhanced the expression of a number of antimicrobial peptides, promoting host innate immunity against microbes and revealing a biological similarity with its mammalian counterpart.
AB - This report describes the cloning and characterisation of rainbow trout (Oncorhynchus mykiss) interleukin (IL)-22, and presents studies of the functional activity of its recombinant protein for the first time in a non-mammalian species. The predicted IL-22 coding region consists of 522 nucleotides which translates into a 173 amino acid protein, that contains an IL-10 family signature which is reasonably well conserved with other vertebrate IL-22 molecules. Expression analysis in tissues from healthy fish revealed a higher constitutive expression of IL-22 in mucosal tissues, suggesting a potentially important role in mucosal immunity. In vitro studies demonstrated that IL-22 expression was induced significantly by PHA and PMA in splenocyte primary cultures 4h post-stimulation. Expression was also induced in the spleen upon infection of fish with the Gram-negative bacterium Yersinia ruckeri, suggesting a potential role of IL-22 in vivo in defence against bacterial diseases. The Escherichia coli produced recombinant IL-22 enhanced the expression of a number of antimicrobial peptides, promoting host innate immunity against microbes and revealing a biological similarity with its mammalian counterpart.
KW - 5' flanking region
KW - amino acid sequence
KW - animals
KW - antimicrobial cationic peptides
KW - base sequence
KW - binding sites
KW - cloning, molecular
KW - gene expression profiling
KW - gene expression regulation
KW - humans
KW - interleukins
KW - introns
KW - molecular sequence data
KW - Oncorhynchus mykiss
KW - phylogeny
KW - protein binding
KW - recombinant proteins
KW - sequence alignment
KW - sequence analysis, DNA
KW - sequence homology, amino acid
KW - spleen
KW - transcription factors
KW - Yersinia ruckeri
KW - interleukin-22
KW - mucosal expression
KW - recombinant IL-22
KW - antimicrobial peptides
U2 - 10.1016/j.cyto.2011.03.015
DO - 10.1016/j.cyto.2011.03.015
M3 - Article
C2 - 21514178
VL - 55
SP - 62
EP - 73
JO - Cytokine
JF - Cytokine
SN - 1043-4666
IS - 1
ER -