Cloning of the Atlantic salmon (Salmo salar) IL-1 receptor associated protein

C Stansberg, S Subramaniam, B Collet, C J Secombes, C Cunningham

Research output: Contribution to journalArticle

Abstract

In mammals, the pro-inflammatory cytokine interleukin-1 signals through a receptor complex containing a type I interleukin-1 receptor (IL-1RI) and a receptor associated protein (IL-1RAcP). Previously, we have described a cDNA from Atlantic salmon encoding a molecule with homology to the mammalian IL-RI. This molecule was named IL-1 receptor like protein (IL-1RLP) in the absence of functional data to support its proposed role as the salmon IL-1RI. Here, we describe the cloning and characterisation. of a cDNA encoding salmon IL-1RAcP. Like other members of the IL-1R family, the salmon IL-1RAcP encodes three extracellular immunoglobulin-like domains and a cytoplasmic Toll/Interleukin-1 receptor (TIR) domain involved in signalling. Specific binding of salmon IL-1RAcP to IL-1RLP was shown by co-immunoprecipitation studies. (c) 2005 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)53-65
Number of pages13
JournalFish & Shellfish Immunology
Volume19
DOIs
Publication statusPublished - 2005

Keywords

  • Salmo salar
  • IL-1R family
  • TIR family
  • IL-1
  • IL-1RLP
  • IL-1RAcP
  • TROUT ONCORHYNCHUS-MYKISS
  • INTERLEUKIN-1 RECEPTOR
  • ACCESSORY PROTEIN
  • IRAK
  • COMPLEX
  • FAMILY
  • IDENTIFICATION
  • EXPRESSION
  • PATHWAY
  • MEMBER

Cite this

Stansberg, C., Subramaniam, S., Collet, B., Secombes, C. J., & Cunningham, C. (2005). Cloning of the Atlantic salmon (Salmo salar) IL-1 receptor associated protein. Fish & Shellfish Immunology, 19, 53-65. https://doi.org/10.1016/j.fsi.2004.11.006