Cloning of two chemokine receptor homologs (CXC-R4 and CC-R7) in rainbow trout Oncorhynchus mykiss

G D Daniels, J Zou, J Charlemagne, S Partula, C Cunningham, C J Secombes

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51 Citations (Scopus)

Abstract

Two rainbow trout chemokine receptors hare been sequenced,,vith homology to CXC-R4 and CC-R7 molecules. The CXC-R4 sequence consisted of 1681 nucleotides, which translated into a stature protein of 357 amino acids, with 80.7% similarity to human CXC-R4. The CC-R7 sequence consisted of 2287 nucleotides, which translated into a 368-amino acid mature protein with 64.5% similarity to human CC-R7, Both sequences contained seven hydrophobic regions, representing the seven transmembrane domains (TM) typical of G-protein-coupled receptors, Extracellular cysteines, transmembrane prolines, and the DRY motif immediately following TM3 were conserved. Phylogenetic tree analysis revealed a tight clustering of trout CXC-R4 with CXC-R3-5 genes. Trout CC-R7 clustered with CC-R6-7 and. CXC-R1-2. Reverse transcriptase-polymerase chain reaction analysis demonstrated a wide tissue distribution of CXC-R4 and CC-R7 message in trout, being present in head-kidney leukocytes, blood, gill, brain, spleen, and liver.

Original languageEnglish
Pages (from-to)684-690
Number of pages7
JournalJournal of Leukocyte Biology
Volume65
Publication statusPublished - 1999

Keywords

  • cDNA cloning
  • reverse transcriptase-polymerase chain reaction
  • CHEMOTACTIC CYTOKINES
  • SEQUENCE ALIGNMENT
  • MOLECULAR-CLONING
  • CC-CHEMOKINES
  • T-CELLS
  • EXPRESSION
  • FAMILY
  • GENE
  • INTERLEUKIN-8
  • INFLAMMATION

Cite this

Daniels, G. D., Zou, J., Charlemagne, J., Partula, S., Cunningham, C., & Secombes, C. J. (1999). Cloning of two chemokine receptor homologs (CXC-R4 and CC-R7) in rainbow trout Oncorhynchus mykiss. Journal of Leukocyte Biology, 65, 684-690.