Abstract
Two rainbow trout chemokine receptors hare been sequenced,,vith homology to CXC-R4 and CC-R7 molecules. The CXC-R4 sequence consisted of 1681 nucleotides, which translated into a stature protein of 357 amino acids, with 80.7% similarity to human CXC-R4. The CC-R7 sequence consisted of 2287 nucleotides, which translated into a 368-amino acid mature protein with 64.5% similarity to human CC-R7, Both sequences contained seven hydrophobic regions, representing the seven transmembrane domains (TM) typical of G-protein-coupled receptors, Extracellular cysteines, transmembrane prolines, and the DRY motif immediately following TM3 were conserved. Phylogenetic tree analysis revealed a tight clustering of trout CXC-R4 with CXC-R3-5 genes. Trout CC-R7 clustered with CC-R6-7 and. CXC-R1-2. Reverse transcriptase-polymerase chain reaction analysis demonstrated a wide tissue distribution of CXC-R4 and CC-R7 message in trout, being present in head-kidney leukocytes, blood, gill, brain, spleen, and liver.
Original language | English |
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Pages (from-to) | 684-690 |
Number of pages | 7 |
Journal | Journal of Leukocyte Biology |
Volume | 65 |
Publication status | Published - 1999 |
Keywords
- cDNA cloning
- reverse transcriptase-polymerase chain reaction
- CHEMOTACTIC CYTOKINES
- SEQUENCE ALIGNMENT
- MOLECULAR-CLONING
- CC-CHEMOKINES
- T-CELLS
- EXPRESSION
- FAMILY
- GENE
- INTERLEUKIN-8
- INFLAMMATION