Cloning of two chemokine receptor homologs (CXC-R4 and CC-R7) in rainbow trout Oncorhynchus mykiss

G D Daniels, J Zou, J Charlemagne, S Partula, C Cunningham, C J Secombes

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Two rainbow trout chemokine receptors hare been sequenced,,vith homology to CXC-R4 and CC-R7 molecules. The CXC-R4 sequence consisted of 1681 nucleotides, which translated into a stature protein of 357 amino acids, with 80.7% similarity to human CXC-R4. The CC-R7 sequence consisted of 2287 nucleotides, which translated into a 368-amino acid mature protein with 64.5% similarity to human CC-R7, Both sequences contained seven hydrophobic regions, representing the seven transmembrane domains (TM) typical of G-protein-coupled receptors, Extracellular cysteines, transmembrane prolines, and the DRY motif immediately following TM3 were conserved. Phylogenetic tree analysis revealed a tight clustering of trout CXC-R4 with CXC-R3-5 genes. Trout CC-R7 clustered with CC-R6-7 and. CXC-R1-2. Reverse transcriptase-polymerase chain reaction analysis demonstrated a wide tissue distribution of CXC-R4 and CC-R7 message in trout, being present in head-kidney leukocytes, blood, gill, brain, spleen, and liver.

Original languageEnglish
Pages (from-to)684-690
Number of pages7
JournalJournal of Leukocyte Biology
Volume65
Publication statusPublished - 1999

Keywords

  • cDNA cloning
  • reverse transcriptase-polymerase chain reaction
  • CHEMOTACTIC CYTOKINES
  • SEQUENCE ALIGNMENT
  • MOLECULAR-CLONING
  • CC-CHEMOKINES
  • T-CELLS
  • EXPRESSION
  • FAMILY
  • GENE
  • INTERLEUKIN-8
  • INFLAMMATION

Cite this

Cloning of two chemokine receptor homologs (CXC-R4 and CC-R7) in rainbow trout Oncorhynchus mykiss. / Daniels, G D ; Zou, J ; Charlemagne, J ; Partula, S ; Cunningham, C ; Secombes, C J .

In: Journal of Leukocyte Biology, Vol. 65, 1999, p. 684-690.

Research output: Contribution to journalArticle

Daniels, GD, Zou, J, Charlemagne, J, Partula, S, Cunningham, C & Secombes, CJ 1999, 'Cloning of two chemokine receptor homologs (CXC-R4 and CC-R7) in rainbow trout Oncorhynchus mykiss', Journal of Leukocyte Biology, vol. 65, pp. 684-690.
Daniels, G D ; Zou, J ; Charlemagne, J ; Partula, S ; Cunningham, C ; Secombes, C J . / Cloning of two chemokine receptor homologs (CXC-R4 and CC-R7) in rainbow trout Oncorhynchus mykiss. In: Journal of Leukocyte Biology. 1999 ; Vol. 65. pp. 684-690.
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abstract = "Two rainbow trout chemokine receptors hare been sequenced,,vith homology to CXC-R4 and CC-R7 molecules. The CXC-R4 sequence consisted of 1681 nucleotides, which translated into a stature protein of 357 amino acids, with 80.7{\%} similarity to human CXC-R4. The CC-R7 sequence consisted of 2287 nucleotides, which translated into a 368-amino acid mature protein with 64.5{\%} similarity to human CC-R7, Both sequences contained seven hydrophobic regions, representing the seven transmembrane domains (TM) typical of G-protein-coupled receptors, Extracellular cysteines, transmembrane prolines, and the DRY motif immediately following TM3 were conserved. Phylogenetic tree analysis revealed a tight clustering of trout CXC-R4 with CXC-R3-5 genes. Trout CC-R7 clustered with CC-R6-7 and. CXC-R1-2. Reverse transcriptase-polymerase chain reaction analysis demonstrated a wide tissue distribution of CXC-R4 and CC-R7 message in trout, being present in head-kidney leukocytes, blood, gill, brain, spleen, and liver.",
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T1 - Cloning of two chemokine receptor homologs (CXC-R4 and CC-R7) in rainbow trout Oncorhynchus mykiss

AU - Daniels, G D

AU - Zou, J

AU - Charlemagne, J

AU - Partula, S

AU - Cunningham, C

AU - Secombes, C J

PY - 1999

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N2 - Two rainbow trout chemokine receptors hare been sequenced,,vith homology to CXC-R4 and CC-R7 molecules. The CXC-R4 sequence consisted of 1681 nucleotides, which translated into a stature protein of 357 amino acids, with 80.7% similarity to human CXC-R4. The CC-R7 sequence consisted of 2287 nucleotides, which translated into a 368-amino acid mature protein with 64.5% similarity to human CC-R7, Both sequences contained seven hydrophobic regions, representing the seven transmembrane domains (TM) typical of G-protein-coupled receptors, Extracellular cysteines, transmembrane prolines, and the DRY motif immediately following TM3 were conserved. Phylogenetic tree analysis revealed a tight clustering of trout CXC-R4 with CXC-R3-5 genes. Trout CC-R7 clustered with CC-R6-7 and. CXC-R1-2. Reverse transcriptase-polymerase chain reaction analysis demonstrated a wide tissue distribution of CXC-R4 and CC-R7 message in trout, being present in head-kidney leukocytes, blood, gill, brain, spleen, and liver.

AB - Two rainbow trout chemokine receptors hare been sequenced,,vith homology to CXC-R4 and CC-R7 molecules. The CXC-R4 sequence consisted of 1681 nucleotides, which translated into a stature protein of 357 amino acids, with 80.7% similarity to human CXC-R4. The CC-R7 sequence consisted of 2287 nucleotides, which translated into a 368-amino acid mature protein with 64.5% similarity to human CC-R7, Both sequences contained seven hydrophobic regions, representing the seven transmembrane domains (TM) typical of G-protein-coupled receptors, Extracellular cysteines, transmembrane prolines, and the DRY motif immediately following TM3 were conserved. Phylogenetic tree analysis revealed a tight clustering of trout CXC-R4 with CXC-R3-5 genes. Trout CC-R7 clustered with CC-R6-7 and. CXC-R1-2. Reverse transcriptase-polymerase chain reaction analysis demonstrated a wide tissue distribution of CXC-R4 and CC-R7 message in trout, being present in head-kidney leukocytes, blood, gill, brain, spleen, and liver.

KW - cDNA cloning

KW - reverse transcriptase-polymerase chain reaction

KW - CHEMOTACTIC CYTOKINES

KW - SEQUENCE ALIGNMENT

KW - MOLECULAR-CLONING

KW - CC-CHEMOKINES

KW - T-CELLS

KW - EXPRESSION

KW - FAMILY

KW - GENE

KW - INTERLEUKIN-8

KW - INFLAMMATION

M3 - Article

VL - 65

SP - 684

EP - 690

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

SN - 0741-5400

ER -