Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains

Mingxian Chang, Tiehui Wang, Pin Nie, Jun Zou, Christopher J. Secombes

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Nucleotide-binding oligomerization domain 2 (NOD2) is a cytoplasmic pattern recognition receptor (PRR), which is involved in innate antibacterial and antiviral responses. Here, two NOD2 splice variants, trNOD2a and trNOD2b, are reported in rainbow trout Oncorhynchus mykiss, that share 63% and 61% similarity with human NOD2, respectively. These two trout NOD2 splice variants were shown to be constitutively expressed in thymus, gills, skin, muscle, liver, spleen, head kidney, intestine, heart, and brain, with the expression of trout NOD2 (trNOD2) mainly contributed by trNOD2a in all the examined tissues. PolyI:C transfection up-regulated the expression of trNOD2a and trNOD2b in RTG-2 cells. The expression of trNOD2a/b was modulated by the inflammatory stimulant interferon-gamma (IFN-gamma) or interleukin-1 beta (IL-1 beta). Overexpression of trout NOD2 effector domains resulted in induced expression of proinflammatory cytokines including IL-1 beta, tumor necrosis factor-alpha (TNF-alpha), IL-6 and IL-8, the antibacterial peptide cathelicidin-2, a variety of caspases including caspase-2, -6, -7, -8, -9, and type I and type II IFN. These results suggest that fish NOD2 functions in inflammatory events, possibly via NF-kappa B activation, regulation of apoptosis, and triggering of antibacterial and antiviral defences. (C) 2010 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)118-127
Number of pages10
JournalFish & Shellfish Immunology
Volume30
Issue number1
Early online date1 Oct 2010
DOIs
Publication statusPublished - Jan 2011

Keywords

  • Amino Acid Sequence
  • Animals
  • Apoptosis
  • Base Sequence
  • Caspases
  • Cytokines
  • Gene Expression Regulation
  • Inflammation
  • Molecular Sequence Data
  • Nod2 Signaling Adaptor Protein
  • Oncorhynchus mykiss
  • Phylogeny
  • Polymerase Chain Reaction
  • Protein Isoforms
  • Protein Structure, Tertiary
  • Rainbow trout
  • Proinflammatory cytokine
  • NOD2
  • NF-KAPPA-B
  • almon salmo-salar
  • Oncorhynchus-mykiss
  • Family-member
  • IFN-gamma
  • Muramyl dipeptide
  • Innate immunity
  • Teleost fish
  • Recognition

Cite this

@article{a0091d6cc6404e5784587c10fcb49742,
title = "Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains",
abstract = "Nucleotide-binding oligomerization domain 2 (NOD2) is a cytoplasmic pattern recognition receptor (PRR), which is involved in innate antibacterial and antiviral responses. Here, two NOD2 splice variants, trNOD2a and trNOD2b, are reported in rainbow trout Oncorhynchus mykiss, that share 63{\%} and 61{\%} similarity with human NOD2, respectively. These two trout NOD2 splice variants were shown to be constitutively expressed in thymus, gills, skin, muscle, liver, spleen, head kidney, intestine, heart, and brain, with the expression of trout NOD2 (trNOD2) mainly contributed by trNOD2a in all the examined tissues. PolyI:C transfection up-regulated the expression of trNOD2a and trNOD2b in RTG-2 cells. The expression of trNOD2a/b was modulated by the inflammatory stimulant interferon-gamma (IFN-gamma) or interleukin-1 beta (IL-1 beta). Overexpression of trout NOD2 effector domains resulted in induced expression of proinflammatory cytokines including IL-1 beta, tumor necrosis factor-alpha (TNF-alpha), IL-6 and IL-8, the antibacterial peptide cathelicidin-2, a variety of caspases including caspase-2, -6, -7, -8, -9, and type I and type II IFN. These results suggest that fish NOD2 functions in inflammatory events, possibly via NF-kappa B activation, regulation of apoptosis, and triggering of antibacterial and antiviral defences. (C) 2010 Elsevier Ltd. All rights reserved.",
keywords = "Amino Acid Sequence, Animals, Apoptosis, Base Sequence, Caspases, Cytokines, Gene Expression Regulation, Inflammation, Molecular Sequence Data, Nod2 Signaling Adaptor Protein, Oncorhynchus mykiss, Phylogeny, Polymerase Chain Reaction, Protein Isoforms, Protein Structure, Tertiary, Rainbow trout , Proinflammatory cytokine, NOD2, NF-KAPPA-B, almon salmo-salar, Oncorhynchus-mykiss, Family-member, IFN-gamma, Muramyl dipeptide, Innate immunity, Teleost fish, Recognition",
author = "Mingxian Chang and Tiehui Wang and Pin Nie and Jun Zou and Secombes, {Christopher J.}",
year = "2011",
month = "1",
doi = "10.1016/j.fsi.2010.09.014",
language = "English",
volume = "30",
pages = "118--127",
journal = "Fish & Shellfish Immunology",
issn = "1050-4648",
publisher = "ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD",
number = "1",

}

TY - JOUR

T1 - Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains

AU - Chang, Mingxian

AU - Wang, Tiehui

AU - Nie, Pin

AU - Zou, Jun

AU - Secombes, Christopher J.

PY - 2011/1

Y1 - 2011/1

N2 - Nucleotide-binding oligomerization domain 2 (NOD2) is a cytoplasmic pattern recognition receptor (PRR), which is involved in innate antibacterial and antiviral responses. Here, two NOD2 splice variants, trNOD2a and trNOD2b, are reported in rainbow trout Oncorhynchus mykiss, that share 63% and 61% similarity with human NOD2, respectively. These two trout NOD2 splice variants were shown to be constitutively expressed in thymus, gills, skin, muscle, liver, spleen, head kidney, intestine, heart, and brain, with the expression of trout NOD2 (trNOD2) mainly contributed by trNOD2a in all the examined tissues. PolyI:C transfection up-regulated the expression of trNOD2a and trNOD2b in RTG-2 cells. The expression of trNOD2a/b was modulated by the inflammatory stimulant interferon-gamma (IFN-gamma) or interleukin-1 beta (IL-1 beta). Overexpression of trout NOD2 effector domains resulted in induced expression of proinflammatory cytokines including IL-1 beta, tumor necrosis factor-alpha (TNF-alpha), IL-6 and IL-8, the antibacterial peptide cathelicidin-2, a variety of caspases including caspase-2, -6, -7, -8, -9, and type I and type II IFN. These results suggest that fish NOD2 functions in inflammatory events, possibly via NF-kappa B activation, regulation of apoptosis, and triggering of antibacterial and antiviral defences. (C) 2010 Elsevier Ltd. All rights reserved.

AB - Nucleotide-binding oligomerization domain 2 (NOD2) is a cytoplasmic pattern recognition receptor (PRR), which is involved in innate antibacterial and antiviral responses. Here, two NOD2 splice variants, trNOD2a and trNOD2b, are reported in rainbow trout Oncorhynchus mykiss, that share 63% and 61% similarity with human NOD2, respectively. These two trout NOD2 splice variants were shown to be constitutively expressed in thymus, gills, skin, muscle, liver, spleen, head kidney, intestine, heart, and brain, with the expression of trout NOD2 (trNOD2) mainly contributed by trNOD2a in all the examined tissues. PolyI:C transfection up-regulated the expression of trNOD2a and trNOD2b in RTG-2 cells. The expression of trNOD2a/b was modulated by the inflammatory stimulant interferon-gamma (IFN-gamma) or interleukin-1 beta (IL-1 beta). Overexpression of trout NOD2 effector domains resulted in induced expression of proinflammatory cytokines including IL-1 beta, tumor necrosis factor-alpha (TNF-alpha), IL-6 and IL-8, the antibacterial peptide cathelicidin-2, a variety of caspases including caspase-2, -6, -7, -8, -9, and type I and type II IFN. These results suggest that fish NOD2 functions in inflammatory events, possibly via NF-kappa B activation, regulation of apoptosis, and triggering of antibacterial and antiviral defences. (C) 2010 Elsevier Ltd. All rights reserved.

KW - Amino Acid Sequence

KW - Animals

KW - Apoptosis

KW - Base Sequence

KW - Caspases

KW - Cytokines

KW - Gene Expression Regulation

KW - Inflammation

KW - Molecular Sequence Data

KW - Nod2 Signaling Adaptor Protein

KW - Oncorhynchus mykiss

KW - Phylogeny

KW - Polymerase Chain Reaction

KW - Protein Isoforms

KW - Protein Structure, Tertiary

KW - Rainbow trout

KW - Proinflammatory cytokine

KW - NOD2

KW - NF-KAPPA-B

KW - almon salmo-salar

KW - Oncorhynchus-mykiss

KW - Family-member

KW - IFN-gamma

KW - Muramyl dipeptide

KW - Innate immunity

KW - Teleost fish

KW - Recognition

U2 - 10.1016/j.fsi.2010.09.014

DO - 10.1016/j.fsi.2010.09.014

M3 - Article

C2 - 20888918

VL - 30

SP - 118

EP - 127

JO - Fish & Shellfish Immunology

JF - Fish & Shellfish Immunology

SN - 1050-4648

IS - 1

ER -