Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains

Mingxian Chang, Tiehui Wang, Pin Nie, Jun Zou, Christopher J. Secombes

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Nucleotide-binding oligomerization domain 2 (NOD2) is a cytoplasmic pattern recognition receptor (PRR), which is involved in innate antibacterial and antiviral responses. Here, two NOD2 splice variants, trNOD2a and trNOD2b, are reported in rainbow trout Oncorhynchus mykiss, that share 63% and 61% similarity with human NOD2, respectively. These two trout NOD2 splice variants were shown to be constitutively expressed in thymus, gills, skin, muscle, liver, spleen, head kidney, intestine, heart, and brain, with the expression of trout NOD2 (trNOD2) mainly contributed by trNOD2a in all the examined tissues. PolyI:C transfection up-regulated the expression of trNOD2a and trNOD2b in RTG-2 cells. The expression of trNOD2a/b was modulated by the inflammatory stimulant interferon-gamma (IFN-gamma) or interleukin-1 beta (IL-1 beta). Overexpression of trout NOD2 effector domains resulted in induced expression of proinflammatory cytokines including IL-1 beta, tumor necrosis factor-alpha (TNF-alpha), IL-6 and IL-8, the antibacterial peptide cathelicidin-2, a variety of caspases including caspase-2, -6, -7, -8, -9, and type I and type II IFN. These results suggest that fish NOD2 functions in inflammatory events, possibly via NF-kappa B activation, regulation of apoptosis, and triggering of antibacterial and antiviral defences. (C) 2010 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)118-127
Number of pages10
JournalFish & Shellfish Immunology
Issue number1
Early online date1 Oct 2010
Publication statusPublished - Jan 2011


  • Amino Acid Sequence
  • Animals
  • Apoptosis
  • Base Sequence
  • Caspases
  • Cytokines
  • Gene Expression Regulation
  • Inflammation
  • Molecular Sequence Data
  • Nod2 Signaling Adaptor Protein
  • Oncorhynchus mykiss
  • Phylogeny
  • Polymerase Chain Reaction
  • Protein Isoforms
  • Protein Structure, Tertiary
  • Rainbow trout
  • Proinflammatory cytokine
  • NOD2
  • almon salmo-salar
  • Oncorhynchus-mykiss
  • Family-member
  • IFN-gamma
  • Muramyl dipeptide
  • Innate immunity
  • Teleost fish
  • Recognition

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