CO2 acts as a signalling molecule in populations of the fungal pathogen Candida albicans

Rebecca Hall, Luisa De Sordi, Donna M MacCallum, Huesnue Topal, Rebecca Eaton, James W Bloor, Gary K Robinson, Lonny R Levin, Jochen Buck, Yue Wang, Neil A R Gow, Clemens Steegborn, Fritz A Muehlschlegel (Corresponding Author)

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Abstract

When colonising host-niches or non-animated medical devices, individual cells of the fungal pathogen Candida albicans expand into significant biomasses. Here we show that within such biomasses, fungal metabolically generated CO2 acts as a communication molecule promoting the switch from yeast to filamentous growth essential for C. albicans pathology. We find that CO2-mediated intra-colony signalling involves the adenylyl cyclase protein (Cyr1p), a multi-sensor recently found to coordinate fungal responses to serum and bacterial peptidoglycan. We further identify Lys 1373 as essential for CO2/bicarbonate regulation of Cyr1p. Disruption of the CO2/bicarbonate receptor-site interferes selectively with C. albicans filamentation within fungal biomasses. Comparisons between the Drosophila melanogaster infection model and the mouse model of disseminated candidiasis, suggest that metabolic CO2 sensing may be important for initial colonisation and epithelial invasion. Our results reveal the existence of a gaseous Candida signalling pathway and its molecular mechanism and provide insights into an evolutionary conserved CO2-signalling system.
Original languageEnglish
Article numbere1001193
Number of pages11
JournalPLoS Pathogens
Volume6
Issue number11
DOIs
Publication statusPublished - 18 Nov 2010

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Candida albicans
Biomass
Bicarbonates
Population
Peptidoglycan
Candidiasis
Drosophila melanogaster
Candida
Adenylyl Cyclases
Yeasts
Communication
Pathology
Equipment and Supplies
Growth
Infection
Serum
Proteins

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Hall, R., De Sordi, L., MacCallum, D. M., Topal, H., Eaton, R., Bloor, J. W., ... Muehlschlegel, F. A. (2010). CO2 acts as a signalling molecule in populations of the fungal pathogen Candida albicans. PLoS Pathogens, 6(11), [e1001193]. https://doi.org/10.1371/journal.ppat.1001193

CO2 acts as a signalling molecule in populations of the fungal pathogen Candida albicans. / Hall, Rebecca; De Sordi, Luisa; MacCallum, Donna M; Topal, Huesnue; Eaton, Rebecca; Bloor, James W; Robinson, Gary K; Levin, Lonny R; Buck, Jochen; Wang, Yue; Gow, Neil A R; Steegborn, Clemens ; Muehlschlegel, Fritz A (Corresponding Author).

In: PLoS Pathogens, Vol. 6, No. 11, e1001193, 18.11.2010.

Research output: Contribution to journalArticle

Hall, R, De Sordi, L, MacCallum, DM, Topal, H, Eaton, R, Bloor, JW, Robinson, GK, Levin, LR, Buck, J, Wang, Y, Gow, NAR, Steegborn, C & Muehlschlegel, FA 2010, 'CO2 acts as a signalling molecule in populations of the fungal pathogen Candida albicans', PLoS Pathogens, vol. 6, no. 11, e1001193. https://doi.org/10.1371/journal.ppat.1001193
Hall, Rebecca ; De Sordi, Luisa ; MacCallum, Donna M ; Topal, Huesnue ; Eaton, Rebecca ; Bloor, James W ; Robinson, Gary K ; Levin, Lonny R ; Buck, Jochen ; Wang, Yue ; Gow, Neil A R ; Steegborn, Clemens ; Muehlschlegel, Fritz A. / CO2 acts as a signalling molecule in populations of the fungal pathogen Candida albicans. In: PLoS Pathogens. 2010 ; Vol. 6, No. 11.
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