Cobalamin Concentrations in Fetal Liver Show Gender Differences: A Result from Using a High-Pressure Liquid Chromatography−Inductively Coupled Plasma Mass Spectrometry as an Ultratrace Cobalt Speciation Method

Janine Bosle, Sven Goetz, Andrea Raab, Eva M. Krupp, Kirk G. Scheckel, Enzo Lombi, Andrew A. Meharg, Paul A. Fowler, Joerg Feldmann

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Abstract

Maternal diet and lifestyle choices may affect placental transfer of cobalamin (Cbl) to the fetus. Fetal liver concentration of Cbl reflects nutritional status with regards to vitamin B12, but at these low concentration current Cbl measurement methods lack robustness. An analytical method based on enzymatic extraction with subsequent reversed-phasehigh-pressure liquid chromatography (RP-HPLC) separation and parallel ICPMS and electrospray ionization (ESI)-Orbitrap-MS to determine specifically Cbl species in liver samples of only 10−50 mg was developed using 14 pig livers. Subsequently 55 human fetal livers were analyzed. HPLC−ICPMS analysis for cobalt (Co) and Cbl gave detection limits of 0.18 ng/g and 0.88 ng/g d.m. in liver samples, respectively, with a recovery of >95%. Total Co (Cot) concentration did not reflect the amount of Cbl or vitamin B12 in the liver. Cbl bound Co contributes only 45 ± 15% to Cot. XRF mapping and μXANES analysis confirmed the occurrence of non-Cbl cobalt in pig liver hot spots indicating particular Co. No correlations of total cobalt nor Cbl with fetal weight or weeks of gestation were found for the human fetal livers. Although no gender difference could be identified for total Co concentration, female livers were significantly higher in Cbl concentration (24.1 ± 7.8 ng/g) than those from male fetuses (19.8 ± 7.1 ng/g) (p = 0.04). This HPLC−ICPMS method was able to quantify total Cot and Cbl in fetus liver, and it was sensitive and precise enough to identify this gender difference.
Original languageEnglish
Pages (from-to)12419–12426
Number of pages8
JournalAnalytical Chemistry
Volume88
Issue number24
Early online date21 Nov 2016
DOIs
Publication statusPublished - 20 Dec 2016

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Vitamin B 12
Cobalt
Liver
Mass spectrometry
Plasmas
Liquids
Electrospray ionization
Nutrition
Chromatography
Recovery

Cite this

@article{27200600c23a467db4700308ba756f59,
title = "Cobalamin Concentrations in Fetal Liver Show Gender Differences: A Result from Using a High-Pressure Liquid Chromatography−Inductively Coupled Plasma Mass Spectrometry as an Ultratrace Cobalt Speciation Method",
abstract = "Maternal diet and lifestyle choices may affect placental transfer of cobalamin (Cbl) to the fetus. Fetal liver concentration of Cbl reflects nutritional status with regards to vitamin B12, but at these low concentration current Cbl measurement methods lack robustness. An analytical method based on enzymatic extraction with subsequent reversed-phasehigh-pressure liquid chromatography (RP-HPLC) separation and parallel ICPMS and electrospray ionization (ESI)-Orbitrap-MS to determine specifically Cbl species in liver samples of only 10−50 mg was developed using 14 pig livers. Subsequently 55 human fetal livers were analyzed. HPLC−ICPMS analysis for cobalt (Co) and Cbl gave detection limits of 0.18 ng/g and 0.88 ng/g d.m. in liver samples, respectively, with a recovery of >95{\%}. Total Co (Cot) concentration did not reflect the amount of Cbl or vitamin B12 in the liver. Cbl bound Co contributes only 45 ± 15{\%} to Cot. XRF mapping and μXANES analysis confirmed the occurrence of non-Cbl cobalt in pig liver hot spots indicating particular Co. No correlations of total cobalt nor Cbl with fetal weight or weeks of gestation were found for the human fetal livers. Although no gender difference could be identified for total Co concentration, female livers were significantly higher in Cbl concentration (24.1 ± 7.8 ng/g) than those from male fetuses (19.8 ± 7.1 ng/g) (p = 0.04). This HPLC−ICPMS method was able to quantify total Cot and Cbl in fetus liver, and it was sensitive and precise enough to identify this gender difference.",
author = "Janine Bosle and Sven Goetz and Andrea Raab and Krupp, {Eva M.} and Scheckel, {Kirk G.} and Enzo Lombi and Meharg, {Andrew A.} and Fowler, {Paul A.} and Joerg Feldmann",
note = "Chief Scientist Office (Scottish Executive, Grant CZG/1/109 (P.A.F.) and Grant CZG/4/742 (P.A.F.); NHS Grampian Endowments 08/02 (P.A.F.); the European Community’s Seventh Framework Programme (Grant FP7/2007-2013) under Grant Agreement No. 212885 (P.A.F.); and the Medical Research Council Grant MR/L010011/1 (P.A.F.).",
year = "2016",
month = "12",
day = "20",
doi = "10.1021/acs.analchem.6b03730",
language = "English",
volume = "88",
pages = "12419–12426",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "AMER CHEMICAL SOC",
number = "24",

}

TY - JOUR

T1 - Cobalamin Concentrations in Fetal Liver Show Gender Differences

T2 - A Result from Using a High-Pressure Liquid Chromatography−Inductively Coupled Plasma Mass Spectrometry as an Ultratrace Cobalt Speciation Method

AU - Bosle, Janine

AU - Goetz, Sven

AU - Raab, Andrea

AU - Krupp, Eva M.

AU - Scheckel, Kirk G.

AU - Lombi, Enzo

AU - Meharg, Andrew A.

AU - Fowler, Paul A.

AU - Feldmann, Joerg

N1 - Chief Scientist Office (Scottish Executive, Grant CZG/1/109 (P.A.F.) and Grant CZG/4/742 (P.A.F.); NHS Grampian Endowments 08/02 (P.A.F.); the European Community’s Seventh Framework Programme (Grant FP7/2007-2013) under Grant Agreement No. 212885 (P.A.F.); and the Medical Research Council Grant MR/L010011/1 (P.A.F.).

PY - 2016/12/20

Y1 - 2016/12/20

N2 - Maternal diet and lifestyle choices may affect placental transfer of cobalamin (Cbl) to the fetus. Fetal liver concentration of Cbl reflects nutritional status with regards to vitamin B12, but at these low concentration current Cbl measurement methods lack robustness. An analytical method based on enzymatic extraction with subsequent reversed-phasehigh-pressure liquid chromatography (RP-HPLC) separation and parallel ICPMS and electrospray ionization (ESI)-Orbitrap-MS to determine specifically Cbl species in liver samples of only 10−50 mg was developed using 14 pig livers. Subsequently 55 human fetal livers were analyzed. HPLC−ICPMS analysis for cobalt (Co) and Cbl gave detection limits of 0.18 ng/g and 0.88 ng/g d.m. in liver samples, respectively, with a recovery of >95%. Total Co (Cot) concentration did not reflect the amount of Cbl or vitamin B12 in the liver. Cbl bound Co contributes only 45 ± 15% to Cot. XRF mapping and μXANES analysis confirmed the occurrence of non-Cbl cobalt in pig liver hot spots indicating particular Co. No correlations of total cobalt nor Cbl with fetal weight or weeks of gestation were found for the human fetal livers. Although no gender difference could be identified for total Co concentration, female livers were significantly higher in Cbl concentration (24.1 ± 7.8 ng/g) than those from male fetuses (19.8 ± 7.1 ng/g) (p = 0.04). This HPLC−ICPMS method was able to quantify total Cot and Cbl in fetus liver, and it was sensitive and precise enough to identify this gender difference.

AB - Maternal diet and lifestyle choices may affect placental transfer of cobalamin (Cbl) to the fetus. Fetal liver concentration of Cbl reflects nutritional status with regards to vitamin B12, but at these low concentration current Cbl measurement methods lack robustness. An analytical method based on enzymatic extraction with subsequent reversed-phasehigh-pressure liquid chromatography (RP-HPLC) separation and parallel ICPMS and electrospray ionization (ESI)-Orbitrap-MS to determine specifically Cbl species in liver samples of only 10−50 mg was developed using 14 pig livers. Subsequently 55 human fetal livers were analyzed. HPLC−ICPMS analysis for cobalt (Co) and Cbl gave detection limits of 0.18 ng/g and 0.88 ng/g d.m. in liver samples, respectively, with a recovery of >95%. Total Co (Cot) concentration did not reflect the amount of Cbl or vitamin B12 in the liver. Cbl bound Co contributes only 45 ± 15% to Cot. XRF mapping and μXANES analysis confirmed the occurrence of non-Cbl cobalt in pig liver hot spots indicating particular Co. No correlations of total cobalt nor Cbl with fetal weight or weeks of gestation were found for the human fetal livers. Although no gender difference could be identified for total Co concentration, female livers were significantly higher in Cbl concentration (24.1 ± 7.8 ng/g) than those from male fetuses (19.8 ± 7.1 ng/g) (p = 0.04). This HPLC−ICPMS method was able to quantify total Cot and Cbl in fetus liver, and it was sensitive and precise enough to identify this gender difference.

U2 - 10.1021/acs.analchem.6b03730

DO - 10.1021/acs.analchem.6b03730

M3 - Article

VL - 88

SP - 12419

EP - 12426

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 24

ER -