Abstract
Maternal diet and lifestyle choices may affect
placental transfer of cobalamin (Cbl) to the fetus. Fetal liver
concentration of Cbl reflects nutritional status with regards to
vitamin B12, but at these low concentration current Cbl
measurement methods lack robustness. An analytical method
based on enzymatic extraction with subsequent reversed-phasehigh-pressure
liquid chromatography (RP-HPLC) separation and
parallel ICPMS and electrospray ionization (ESI)-Orbitrap-MS to
determine specifically Cbl species in liver samples of only 10−50
mg was developed using 14 pig livers. Subsequently 55 human
fetal livers were analyzed. HPLC−ICPMS analysis for cobalt (Co)
and Cbl gave detection limits of 0.18 ng/g and 0.88 ng/g d.m. in
liver samples, respectively, with a recovery of >95%. Total Co
(Cot) concentration did not reflect the amount of Cbl or vitamin
B12 in the liver. Cbl bound Co contributes only 45 ± 15% to Cot. XRF mapping and μXANES analysis confirmed the occurrence
of non-Cbl cobalt in pig liver hot spots indicating particular Co. No correlations of total cobalt nor Cbl with fetal weight or weeks
of gestation were found for the human fetal livers. Although no gender difference could be identified for total Co concentration,
female livers were significantly higher in Cbl concentration (24.1 ± 7.8 ng/g) than those from male fetuses (19.8 ± 7.1 ng/g) (p
= 0.04). This HPLC−ICPMS method was able to quantify total Cot and Cbl in fetus liver, and it was sensitive and precise
enough to identify this gender difference.
Original language | English |
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Pages (from-to) | 12419–12426 |
Number of pages | 8 |
Journal | Analytical Chemistry |
Volume | 88 |
Issue number | 24 |
Early online date | 21 Nov 2016 |
DOIs | |
Publication status | Published - 20 Dec 2016 |
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Dive into the research topics of 'Cobalamin Concentrations in Fetal Liver Show Gender Differences: A Result from Using a High-Pressure Liquid Chromatography−Inductively Coupled Plasma Mass Spectrometry as an Ultratrace Cobalt Speciation Method'. Together they form a unique fingerprint.Profiles
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Paul Alfred François Fowler
- School of Medicine, Medical Sciences & Nutrition, Applied Medicine - Chair in Translational Medical Sciences
- Institute of Medical Sciences
Person: Academic