Colitis susceptibility in mice with reactive oxygen species deficiency is mediated by mucus barrier and immune defense defects

Gabriella Aviello, Ashish K. Singh, Sharon O’Neill, Emer Conroy, William Gallagher, Giuseppe D’Agostino, Alan W. Walker, Billy Bourke, Dimitri Scholz, Ulla G. Knaus (Corresponding Author)

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) generated by NADPH oxidases (NOX/DUOX) provide antimicrobial defense, redox signaling, and gut barrier maintenance. Inactivating NOX variants are associated with comorbid intestinal inflammation in chronic granulomatous disease (CGD; NOX2) and pediatric inflammatory bowel disease (IBD; NOX1); however Nox-deficient mice do not reflect human disease susceptibility. Here we assessed if a hypomorphic patient-relevant CGD mutation will increase the risk for intestinal inflammation in mice. Cyba (p22phox) mutant mice generated low intestinal ROS, while maintaining Nox4 function. The Cyba variant caused profound mucus layer disruption with bacterial penetration into crypts, dysbiosis, and a compromised innate immune response to invading microbes, leading to mortality. Approaches used in treatment-resistant CGD or pediatric IBD such as bone marrow transplantation or oral antibiotic treatment ameliorated or prevented disease in mice. The Cyba mutant mouse phenotype implicates loss of both mucus barrier and efficient innate immune defense in the pathogenesis of intestinal inflammation due to ROS deficiency, supporting a combined-hit model where a single disease variant compromises different cellular functions in interdependent compartments.
Original languageEnglish
Pages (from-to)1316-1326
Number of pages11
JournalMucosal Immunology
Volume12
Early online date25 Sep 2019
DOIs
Publication statusPublished - Nov 2019

Keywords

  • CHRONIC GRANULOMATOUS-DISEASE
  • INFLAMMATORY-BOWEL-DISEASE
  • ENCODING P22(PHOX)
  • DUOX2
  • CYBA
  • EXPRESSION
  • MUTATIONS
  • NOX1
  • HOMEOSTASIS
  • VARIANTS

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