Colorectal cancer genomics: evidence for multiple genotypes which influence survival

Patrick Hugh Rooney, A. Boonsong, J. A. McKay, S. Marsh, David A J Stevenson, Graeme Ian Murray, Stephanie Curran, Neva Elizabeth Haites, J. Cassidy

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Abstract

Colorectal cancer (CRC) is a leading cause of cancer death and the mechanism for variable outcome in this disease is not yet fully understood. It is hypothesized that differences in the genetic make-up of tumours may be partially responsible for the differences observed in survival among same staged individuals for this disease. In this study the tumour genomes of 29 consecutive patients undergoing surgery for Dukes'C CRC were assessed by comparative genomic hybridization (CGH). In addition, the CGH profiles from the tumours were compared with those from eight colorectal cell lines. Great variation in genetic grade (all detectable aberrations i.e., loss + gain) was observed in 29 Dukes' C colorectal tumours by CGH (median four aberrations per tumour, range 0-20). Gain was found in 76% and loss in 41% of tumours. The most frequently observed regions of gain were 13q (27.6%), 20q (27.6%), 7p (24.1%), 8q (24.1%), and Iq (20.7%) and loss were 18q (31%), 4q (20.7%), 17p (20.7%), 18p (20.7%), and 15q (20.1%). None of these specific genomic aberrations were associated with patient survival. However, patients with more than two aberrations had a better survival than patients with fewer regions of loss and gain (P = 0.02). CRC cell lines had similar regions of loss or gain as the tumours. However. the frequency of genomic aberrations was much greater in the CRC cell lines. Although genomic change in CRC is relevant to the survival of patients with Dukes'C CRC, careful analysis is required to identify cell lines which are representative models of CRC genomics. (C) 2001 Cancer Research Campaign.

Original languageEnglish
Pages (from-to)1492-1498
Number of pages6
JournalBritish Journal of Cancer
Volume85
Issue number10
DOIs
Publication statusPublished - 2001

Keywords

  • CGH
  • colo-rectal tumours
  • cell lines
  • survival
  • cancer genomics
  • HYBRIDIZATION ANALYSIS
  • SOLID TUMORS
  • INSTABILITY
  • CARCINOMAS
  • GENES

Cite this

Rooney, P. H., Boonsong, A., McKay, J. A., Marsh, S., Stevenson, D. A. J., Murray, G. I., Curran, S., Haites, N. E., & Cassidy, J. (2001). Colorectal cancer genomics: evidence for multiple genotypes which influence survival. British Journal of Cancer, 85(10), 1492-1498. https://doi.org/10.1054/bjoc.2001.2095