Combined ATR-SEIRAS and EC-STM study of the immobilization of laccase on chemically modified Au electrodes

Cristina Vaz-Dominguez, Marcos Pita, Antonio L. de Lacey, Sergey Shleev, Angel Cuesta

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The successive steps of laccase immobilization on chemically modified Au electrodes were monitored using ATR-SEIRAS and in situ STM. Successful covalent immobilization of the enzyme on Au electrodes modified by a mixed aminophenyl-mercaptohexanol adlayer and on Au electrodes modified by a 4-aminothiophenyl SAM via a Schiff base reaction followed by the formation of amide bonds is revealed by the emergence of the corresponding bands in the ATR-SEIRA spectra, and an enzyme coverage on aminophenyl-mercaptohexanol-modified Au electrodes of about (7.27 ± 1.93) × 1011 laccase units per cm2 was calculated from STM images. The small differences between the ATR-SEIRA spectra of the enzyme immobilized on aminophenyl-mercaptohexanol-modified Au electrodes and the ATR-SEIRA spectra of the enzyme immobilized on 4-aminothiophenyl-modified Au electrodes are attributed to a different orientation of the immobilized enzyme due to the presence on the surface of aminophenyl-mercaptohexanol-modified Au electrodes of OH functional groups that favor an orientation of laccase with the Cu T1 center of the enzyme facing the electrode surface, thus, allowing a high activity for direct electrocatalysis of the ORR at low overpotentials.
Original languageEnglish
Pages (from-to)16532-16540
Number of pages9
JournalThe Journal of Physical Chemistry C
Volume116
Issue number31
Early online date16 Jul 2012
DOIs
Publication statusPublished - 9 Aug 2012

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Laccase
immobilization
enzymes
Electrodes
electrodes
Enzymes
Immobilized Enzymes
Electrocatalysis
Schiff Bases
Amides
Functional groups
imines
amides

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Combined ATR-SEIRAS and EC-STM study of the immobilization of laccase on chemically modified Au electrodes. / Vaz-Dominguez, Cristina; Pita, Marcos; de Lacey, Antonio L.; Shleev, Sergey; Cuesta, Angel.

In: The Journal of Physical Chemistry C, Vol. 116, No. 31, 09.08.2012, p. 16532-16540.

Research output: Contribution to journalArticle

Vaz-Dominguez, Cristina ; Pita, Marcos ; de Lacey, Antonio L. ; Shleev, Sergey ; Cuesta, Angel. / Combined ATR-SEIRAS and EC-STM study of the immobilization of laccase on chemically modified Au electrodes. In: The Journal of Physical Chemistry C. 2012 ; Vol. 116, No. 31. pp. 16532-16540.
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