Comparative analysis of sequences flanking tet(W) resistance genes in multiple species of gut bacteria

Katarzyna A Kazimierczak, Harry James Flint, Karen Patricia Scott (Corresponding Author)

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Abstract

tet(W) is one of the most abundant tetracycline resistance genes found in bacteria from the mammalian gut and was first identified in the rumen anaerobe Butyrivibrio fibrisolvens 1.230, where it is highly mobile and its transfer is associated with the transposable chromosomal element TnB1230. In order to compare the genetic basis for tet(W) carriage in different bacteria, we studied sequences flanking tet(W) in representatives of seven bacterial genera originating in diverse gut environments. The sequences 657 bp upstream and 43 bp downstream of tet(W) were 96 to 100% similar in all strains examined. A common open reading frame (ORF) was identified downstream of tet(W) in five different bacteria, while another conserved ORF that flanked tet(W) in B. fibrisolvens 1.230 was also present upstream of tet(W) in a human colonic Roseburia isolate and in another rumen B. fibrisolvens isolate. In one species, Bifidobacterium longum (strain 178), a novel transposase was located within the conserved 657-bp region upstream of tet(W) and was flanked by imperfect direct repeats. Additional direct repeats 6 bp long were identified on each end of a chromosomal ORF interrupted by the insertion of the putative transposase and the tet(W) gene. This tet(W) gene was transferable at low frequencies between Bi dobacterium strains. A putative minielement carrying a copy of tet(W) was identified in B.fibrisolvens transconjugants that had acquired the tet(W) gene on TnB1230. Several different mechanisms, including mechanisms involving plasmids and conjugative transposons, appear to be involved in the horizontal transfer of tet(W) genes, but small core regions that may function as minielements are conserved.

Original languageEnglish
Pages (from-to)2632-2639
Number of pages8
JournalAntimicrobial Agents and Chemotherapy
Volume50
Issue number8
DOIs
Publication statusPublished - Aug 2006

Keywords

  • Anaerobe butyrivibrio fibrisolvens
  • ribosomal protection proteins
  • Megasphaera elsdenii strains
  • tetracycline resistance
  • Selenomonas ruminantium
  • insertion sequences
  • rumen
  • epidemiology
  • enterococcus
  • transposons

Cite this

@article{e6c1d151ed6c45539637fee30bd8bed1,
title = "Comparative analysis of sequences flanking tet(W) resistance genes in multiple species of gut bacteria",
abstract = "tet(W) is one of the most abundant tetracycline resistance genes found in bacteria from the mammalian gut and was first identified in the rumen anaerobe Butyrivibrio fibrisolvens 1.230, where it is highly mobile and its transfer is associated with the transposable chromosomal element TnB1230. In order to compare the genetic basis for tet(W) carriage in different bacteria, we studied sequences flanking tet(W) in representatives of seven bacterial genera originating in diverse gut environments. The sequences 657 bp upstream and 43 bp downstream of tet(W) were 96 to 100{\%} similar in all strains examined. A common open reading frame (ORF) was identified downstream of tet(W) in five different bacteria, while another conserved ORF that flanked tet(W) in B. fibrisolvens 1.230 was also present upstream of tet(W) in a human colonic Roseburia isolate and in another rumen B. fibrisolvens isolate. In one species, Bifidobacterium longum (strain 178), a novel transposase was located within the conserved 657-bp region upstream of tet(W) and was flanked by imperfect direct repeats. Additional direct repeats 6 bp long were identified on each end of a chromosomal ORF interrupted by the insertion of the putative transposase and the tet(W) gene. This tet(W) gene was transferable at low frequencies between Bi dobacterium strains. A putative minielement carrying a copy of tet(W) was identified in B.fibrisolvens transconjugants that had acquired the tet(W) gene on TnB1230. Several different mechanisms, including mechanisms involving plasmids and conjugative transposons, appear to be involved in the horizontal transfer of tet(W) genes, but small core regions that may function as minielements are conserved.",
keywords = "Anaerobe butyrivibrio fibrisolvens, ribosomal protection proteins, Megasphaera elsdenii strains, tetracycline resistance, Selenomonas ruminantium, insertion sequences, rumen, epidemiology, enterococcus, transposons",
author = "Kazimierczak, {Katarzyna A} and Flint, {Harry James} and Scott, {Karen Patricia}",
year = "2006",
month = "8",
doi = "10.1128/AAC.01587-05",
language = "English",
volume = "50",
pages = "2632--2639",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "AMER SOC MICROBIOLOGY",
number = "8",

}

TY - JOUR

T1 - Comparative analysis of sequences flanking tet(W) resistance genes in multiple species of gut bacteria

AU - Kazimierczak, Katarzyna A

AU - Flint, Harry James

AU - Scott, Karen Patricia

PY - 2006/8

Y1 - 2006/8

N2 - tet(W) is one of the most abundant tetracycline resistance genes found in bacteria from the mammalian gut and was first identified in the rumen anaerobe Butyrivibrio fibrisolvens 1.230, where it is highly mobile and its transfer is associated with the transposable chromosomal element TnB1230. In order to compare the genetic basis for tet(W) carriage in different bacteria, we studied sequences flanking tet(W) in representatives of seven bacterial genera originating in diverse gut environments. The sequences 657 bp upstream and 43 bp downstream of tet(W) were 96 to 100% similar in all strains examined. A common open reading frame (ORF) was identified downstream of tet(W) in five different bacteria, while another conserved ORF that flanked tet(W) in B. fibrisolvens 1.230 was also present upstream of tet(W) in a human colonic Roseburia isolate and in another rumen B. fibrisolvens isolate. In one species, Bifidobacterium longum (strain 178), a novel transposase was located within the conserved 657-bp region upstream of tet(W) and was flanked by imperfect direct repeats. Additional direct repeats 6 bp long were identified on each end of a chromosomal ORF interrupted by the insertion of the putative transposase and the tet(W) gene. This tet(W) gene was transferable at low frequencies between Bi dobacterium strains. A putative minielement carrying a copy of tet(W) was identified in B.fibrisolvens transconjugants that had acquired the tet(W) gene on TnB1230. Several different mechanisms, including mechanisms involving plasmids and conjugative transposons, appear to be involved in the horizontal transfer of tet(W) genes, but small core regions that may function as minielements are conserved.

AB - tet(W) is one of the most abundant tetracycline resistance genes found in bacteria from the mammalian gut and was first identified in the rumen anaerobe Butyrivibrio fibrisolvens 1.230, where it is highly mobile and its transfer is associated with the transposable chromosomal element TnB1230. In order to compare the genetic basis for tet(W) carriage in different bacteria, we studied sequences flanking tet(W) in representatives of seven bacterial genera originating in diverse gut environments. The sequences 657 bp upstream and 43 bp downstream of tet(W) were 96 to 100% similar in all strains examined. A common open reading frame (ORF) was identified downstream of tet(W) in five different bacteria, while another conserved ORF that flanked tet(W) in B. fibrisolvens 1.230 was also present upstream of tet(W) in a human colonic Roseburia isolate and in another rumen B. fibrisolvens isolate. In one species, Bifidobacterium longum (strain 178), a novel transposase was located within the conserved 657-bp region upstream of tet(W) and was flanked by imperfect direct repeats. Additional direct repeats 6 bp long were identified on each end of a chromosomal ORF interrupted by the insertion of the putative transposase and the tet(W) gene. This tet(W) gene was transferable at low frequencies between Bi dobacterium strains. A putative minielement carrying a copy of tet(W) was identified in B.fibrisolvens transconjugants that had acquired the tet(W) gene on TnB1230. Several different mechanisms, including mechanisms involving plasmids and conjugative transposons, appear to be involved in the horizontal transfer of tet(W) genes, but small core regions that may function as minielements are conserved.

KW - Anaerobe butyrivibrio fibrisolvens

KW - ribosomal protection proteins

KW - Megasphaera elsdenii strains

KW - tetracycline resistance

KW - Selenomonas ruminantium

KW - insertion sequences

KW - rumen

KW - epidemiology

KW - enterococcus

KW - transposons

U2 - 10.1128/AAC.01587-05

DO - 10.1128/AAC.01587-05

M3 - Article

VL - 50

SP - 2632

EP - 2639

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 8

ER -