Comparative analysis of the excretory-secretory proteome of the muscle larva of Trichinella pseudospiralis and Trichinella spiralis

Mark William Robinson, Rachel Greig, Kenneth A. Beattie, Douglas J. Lamont, Bernadette Connolly

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The nematodes Trichinella spiralis and Trichinella pseudospiralis are both intracellular parasites of skeletal muscle cells and induce profound alterations in the host cell resulting in a re-alignment of muscle-specific gene expression. While T spiralis induces the production of a collagen capsule surrounding the host-parasite complex, T pseudospiralis exists in a non-encapsulated form and is also characterised by suppression of the host inflammatory response in the muscle. These observed differences between the two species are thought to be due to variation in the proteins excreted or secreted (ES proteins) by the muscle larva. In this study, we use a global proteomics approach to compare the ES protein profiles from both species and to identify individual T pseudospiralis proteins that complement earlier studies with T spiralis. Following two-dimensional gel electrophoresis, tandem mass spectrometry was used to identify the pepticle spots. In many cases identification was aided by the determination of partial peptide sequence from selected mass ions. The T pseudospiralis spots identified included the major secreted glycoproteins and the secreted 5'-nucleotidase. Furthermore, two major groups of T spiralis-specific proteins and several T pseudospiralis-specific proteins were identified. Our results demonstrate the value of proteomics as a too] for the identification of ES proteins that are differentially expressed between Trichinella species and as an aid to identifying key parasite proteins that are involved in the host-parasite interaction. The value of this approach will be further enhanced by data arising out the current T spiralis genome sequencing project.

Original languageEnglish
Pages (from-to)139-148
Number of pages10
JournalInternational Journal for Parasitology
Volume37
Issue number2
Early online date15 Sep 2006
DOIs
Publication statusPublished - Feb 2007

Keywords

  • nematode
  • Trichinella
  • muscle larva
  • mass spectrometry
  • ES protein
  • putative serine-protease
  • skeletal-muscle
  • nurse cell
  • excretory/secretory products
  • differential expression
  • monoclonal-antibodies
  • ,ass-spectrometry
  • molecular-cloning
  • rapid expulsion
  • T-spiralis

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