Comparative transcript profiling of Candida albicans and Candida dubliniensis identifies SFL2, a C. albicans gene required for virulence in a reconstituted epithelial infection model

Martin J Spiering, Gary P Moran, Murielle Chauvel, Donna Margaret MacCallum, Judy Higgins, Karsten Hokamp, Tim Yeomans, Christophe d'Enfert, David C Coleman, Jonathan Sullivan

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Abstract

Candida albicans and Candida dubliniensis are closely related species displaying differences in virulence and genome content, therefore providing potential opportunities to identify novel C. albicans virulence genes. C. albicans gene arrays were used for comparative analysis of global gene expression in the two species in reconstituted human oral epithelium (RHE). C. albicans (SC5314) showed upregulation of hypha-specific and virulence genes within 30 min postinoculation, coinciding with rapid induction of filamentation and increased RHE damage. C. dubliniensis (CD36) showed no detectable upregulation of hypha-specific genes, grew as yeast, and caused limited RHE damage. Several genes absent or highly divergent in C. dubliniensis were upregulated in C. albicans. One such gene, SFL2 (orf19.3969), encoding a putative heat shock factor, was deleted in C. albicans. DeltaDeltasfl2 cells failed to filament under a range of hypha-inducing conditions and exhibited greatly reduced RHE damage, reversed by reintroduction of SFL2 into the DeltaDeltasfl2 strain. Moreover, SFL2 overexpression in C. albicans triggered hyphal morphogenesis. Although SFL2 deletion had no apparent effect on host survival in the murine model of systemic infection, DeltaDeltasfl2 strain-infected kidney tissues contained only yeast cells. These results suggest a role for SFL2 in morphogenesis and an indirect role in C. albicans pathogenesis in epithelial tissues.

Original languageEnglish
Pages (from-to)251-265
Number of pages15
JournalEukaryotic Cell
Volume9
Issue number2
Early online date18 Dec 2009
DOIs
Publication statusPublished - Feb 2010

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Candida albicans
Candida
Virulence
Infection
Epithelium
Genes
Hyphae
Morphogenesis
Up-Regulation
Yeasts
Shock
Hot Temperature
Genome
Kidney
Gene Expression
Survival

Cite this

Comparative transcript profiling of Candida albicans and Candida dubliniensis identifies SFL2, a C. albicans gene required for virulence in a reconstituted epithelial infection model. / Spiering, Martin J; Moran, Gary P; Chauvel, Murielle; MacCallum, Donna Margaret; Higgins, Judy; Hokamp, Karsten; Yeomans, Tim; d'Enfert, Christophe; Coleman, David C; Sullivan, Jonathan.

In: Eukaryotic Cell, Vol. 9, No. 2, 02.2010, p. 251-265.

Research output: Contribution to journalArticle

Spiering, Martin J ; Moran, Gary P ; Chauvel, Murielle ; MacCallum, Donna Margaret ; Higgins, Judy ; Hokamp, Karsten ; Yeomans, Tim ; d'Enfert, Christophe ; Coleman, David C ; Sullivan, Jonathan. / Comparative transcript profiling of Candida albicans and Candida dubliniensis identifies SFL2, a C. albicans gene required for virulence in a reconstituted epithelial infection model. In: Eukaryotic Cell. 2010 ; Vol. 9, No. 2. pp. 251-265.
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