Comparison of methods to investigate microbial populations in soils under different agricultural management

K Lawlor, B P Knight, V L Barbosa-Jefferson, P W Lane, A K Lilley, G I Paton, S P McGrath, S M O'Flaherty, P R Hirsch

Research output: Contribution to journalArticle

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Abstract

The microbial community in sludge-treated and nearby untreated soils was investigated using direct microscopic counting, plate culture, carbon substrate utilisation (Biolog (TM)), and fatty acid methyl esters. Long-term sewage sludge applications had resulted in higher concentrations of organic carbon and had altered other factors in the soil, including the concentration of metals. Bioluminescence was inhibited in assays of the sludge-treated soil, although microbial counts were similar in all soils. A detailed analysis of carbon substrate utilisation patterns and fatty acid methyl esters showed qualitative differences in the microbial populations. This work shows that a variety of approaches are required to assess microbial communities in soil where, despite large differences in land management, the populations are similar in size and overall composition. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)129-137
Number of pages9
JournalFEMS Microbiology Ecology
Volume33
Publication statusPublished - 2000

Keywords

  • soil microbial population
  • Biolog (TM)
  • fatty acid methyl ester
  • bioluminescence
  • sewage sludge
  • FATTY-ACID ANALYSIS
  • COMMUNITY STRUCTURE
  • PSEUDOMONAS-FLUORESCENS
  • SUBSTRATE UTILIZATION
  • HEAVY-METALS
  • CONTAMINATION
  • BIOMASS
  • FORESTS
  • BIOLOGY
  • SHIFTS

Cite this

Lawlor, K., Knight, B. P., Barbosa-Jefferson, V. L., Lane, P. W., Lilley, A. K., Paton, G. I., ... Hirsch, P. R. (2000). Comparison of methods to investigate microbial populations in soils under different agricultural management. FEMS Microbiology Ecology, 33, 129-137.

Comparison of methods to investigate microbial populations in soils under different agricultural management. / Lawlor, K ; Knight, B P ; Barbosa-Jefferson, V L ; Lane, P W ; Lilley, A K ; Paton, G I ; McGrath, S P ; O'Flaherty, S M ; Hirsch, P R .

In: FEMS Microbiology Ecology, Vol. 33, 2000, p. 129-137.

Research output: Contribution to journalArticle

Lawlor, K, Knight, BP, Barbosa-Jefferson, VL, Lane, PW, Lilley, AK, Paton, GI, McGrath, SP, O'Flaherty, SM & Hirsch, PR 2000, 'Comparison of methods to investigate microbial populations in soils under different agricultural management', FEMS Microbiology Ecology, vol. 33, pp. 129-137.
Lawlor, K ; Knight, B P ; Barbosa-Jefferson, V L ; Lane, P W ; Lilley, A K ; Paton, G I ; McGrath, S P ; O'Flaherty, S M ; Hirsch, P R . / Comparison of methods to investigate microbial populations in soils under different agricultural management. In: FEMS Microbiology Ecology. 2000 ; Vol. 33. pp. 129-137.
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AU - Knight, B P

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AU - Lane, P W

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AU - Paton, G I

AU - McGrath, S P

AU - O'Flaherty, S M

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AB - The microbial community in sludge-treated and nearby untreated soils was investigated using direct microscopic counting, plate culture, carbon substrate utilisation (Biolog (TM)), and fatty acid methyl esters. Long-term sewage sludge applications had resulted in higher concentrations of organic carbon and had altered other factors in the soil, including the concentration of metals. Bioluminescence was inhibited in assays of the sludge-treated soil, although microbial counts were similar in all soils. A detailed analysis of carbon substrate utilisation patterns and fatty acid methyl esters showed qualitative differences in the microbial populations. This work shows that a variety of approaches are required to assess microbial communities in soil where, despite large differences in land management, the populations are similar in size and overall composition. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

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KW - Biolog (TM)

KW - fatty acid methyl ester

KW - bioluminescence

KW - sewage sludge

KW - FATTY-ACID ANALYSIS

KW - COMMUNITY STRUCTURE

KW - PSEUDOMONAS-FLUORESCENS

KW - SUBSTRATE UTILIZATION

KW - HEAVY-METALS

KW - CONTAMINATION

KW - BIOMASS

KW - FORESTS

KW - BIOLOGY

KW - SHIFTS

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JO - FEMS Microbiology Ecology

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SN - 1574-6941

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