Abstract
The microbial community in sludge-treated and nearby untreated soils was investigated using direct microscopic counting, plate culture, carbon substrate utilisation (Biolog (TM)), and fatty acid methyl esters. Long-term sewage sludge applications had resulted in higher concentrations of organic carbon and had altered other factors in the soil, including the concentration of metals. Bioluminescence was inhibited in assays of the sludge-treated soil, although microbial counts were similar in all soils. A detailed analysis of carbon substrate utilisation patterns and fatty acid methyl esters showed qualitative differences in the microbial populations. This work shows that a variety of approaches are required to assess microbial communities in soil where, despite large differences in land management, the populations are similar in size and overall composition. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 129-137 |
| Number of pages | 9 |
| Journal | FEMS Microbiology Ecology |
| Volume | 33 |
| Publication status | Published - 2000 |
Keywords
- soil microbial population
- Biolog (TM)
- fatty acid methyl ester
- bioluminescence
- sewage sludge
- FATTY-ACID ANALYSIS
- COMMUNITY STRUCTURE
- PSEUDOMONAS-FLUORESCENS
- SUBSTRATE UTILIZATION
- HEAVY-METALS
- CONTAMINATION
- BIOMASS
- FORESTS
- BIOLOGY
- SHIFTS
Cite this
Comparison of methods to investigate microbial populations in soils under different agricultural management. / Lawlor, K ; Knight, B P ; Barbosa-Jefferson, V L ; Lane, P W ; Lilley, A K ; Paton, G I ; McGrath, S P ; O'Flaherty, S M ; Hirsch, P R .
In: FEMS Microbiology Ecology, Vol. 33, 2000, p. 129-137.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of methods to investigate microbial populations in soils under different agricultural management
AU - Lawlor, K
AU - Knight, B P
AU - Barbosa-Jefferson, V L
AU - Lane, P W
AU - Lilley, A K
AU - Paton, G I
AU - McGrath, S P
AU - O'Flaherty, S M
AU - Hirsch, P R
PY - 2000
Y1 - 2000
N2 - The microbial community in sludge-treated and nearby untreated soils was investigated using direct microscopic counting, plate culture, carbon substrate utilisation (Biolog (TM)), and fatty acid methyl esters. Long-term sewage sludge applications had resulted in higher concentrations of organic carbon and had altered other factors in the soil, including the concentration of metals. Bioluminescence was inhibited in assays of the sludge-treated soil, although microbial counts were similar in all soils. A detailed analysis of carbon substrate utilisation patterns and fatty acid methyl esters showed qualitative differences in the microbial populations. This work shows that a variety of approaches are required to assess microbial communities in soil where, despite large differences in land management, the populations are similar in size and overall composition. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
AB - The microbial community in sludge-treated and nearby untreated soils was investigated using direct microscopic counting, plate culture, carbon substrate utilisation (Biolog (TM)), and fatty acid methyl esters. Long-term sewage sludge applications had resulted in higher concentrations of organic carbon and had altered other factors in the soil, including the concentration of metals. Bioluminescence was inhibited in assays of the sludge-treated soil, although microbial counts were similar in all soils. A detailed analysis of carbon substrate utilisation patterns and fatty acid methyl esters showed qualitative differences in the microbial populations. This work shows that a variety of approaches are required to assess microbial communities in soil where, despite large differences in land management, the populations are similar in size and overall composition. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
KW - soil microbial population
KW - Biolog (TM)
KW - fatty acid methyl ester
KW - bioluminescence
KW - sewage sludge
KW - FATTY-ACID ANALYSIS
KW - COMMUNITY STRUCTURE
KW - PSEUDOMONAS-FLUORESCENS
KW - SUBSTRATE UTILIZATION
KW - HEAVY-METALS
KW - CONTAMINATION
KW - BIOMASS
KW - FORESTS
KW - BIOLOGY
KW - SHIFTS
M3 - Article
VL - 33
SP - 129
EP - 137
JO - FEMS Microbiology Ecology
JF - FEMS Microbiology Ecology
SN - 1574-6941
ER -