Comparison of screening methods for TEM- and SHV-derived extended-spectrum beta-lactamase detection

Fiona MacKenzie, C. A. Miller, Ian M Gould

    Research output: Contribution to journalArticle

    27 Citations (Scopus)

    Abstract

    Objective To compare common extended-spectrum beta-lactamase (ESBL) screening methods and beta-lactams for their ability to detect TEM- and SHV-related ESBL enzymes.

    Methods This study compared disk diffusion testing by NCCLS methodology, the Jarlier double disk test, a disk-on-disk test, a modified three-dimensional test and the E test method for their sensitivity and specificity in detecting TEM- and SHV-related ESBL producers. Three negative and 22 positive controls were studied. These were two Klebsiella pneumoniae and 23 Escherichia coli transconjugants. Seventeen beta-lactam antibiotics were tested: cefamandole, cefotetan, cefoxitin, cefuroxime, cefixime, cefoperazone, cefotaxime, cefpodoxime, cefsulodin, ceftazidime, ceftibuten, ceftizoxime, ceftriaxone, moxalactam, cefepime, cefpirome and aztreonam.

    Results NCCLS disk diffusion was 14% sensitive with ceftriaxone, 36% with cefotaxime, 64% with aztreonam, 68% with cefpodoxime, and 73% with ceftazidime. Cefoperazone, cefamandole, cefpodoxime and cefpirome showed 91% sensitivity using the Jarlier test. Using the disk-on-disk test, cefsulodin showed 95% sensitivity, and cefoperazone, cefepime and cefamandole showed 91% sensitivity. With the modified three-dimensional test, cefoperazone, cefpodoxime and cefpirome showed 91% sensitivity.

    Conclusions For practical reasons, we would recommend use of either the Jarlier test or the commercial cephalosporin disks containing clavulanic acid to screen for ESBL producers. Cefoperazone, cefamandole, cefpodoxime and cefpirome showed good sensitivity across the methods tested.

    Original languageEnglish
    Pages (from-to)715-724
    Number of pages9
    JournalClinical Microbiology and Infection
    Volume8
    Issue number11
    DOIs
    Publication statusPublished - 2002

    Keywords

    • RESISTANT KLEBSIELLA-PNEUMONIAE
    • FAMILY ENTEROBACTERIACEAE
    • ESCHERICHIA-COLI
    • EPIDEMIOLOGY
    • PREVALENCE
    • OUTBREAK
    • MEMBERS
    • DISK

    Cite this

    Comparison of screening methods for TEM- and SHV-derived extended-spectrum beta-lactamase detection. / MacKenzie, Fiona; Miller, C. A.; Gould, Ian M.

    In: Clinical Microbiology and Infection, Vol. 8, No. 11, 2002, p. 715-724.

    Research output: Contribution to journalArticle

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    title = "Comparison of screening methods for TEM- and SHV-derived extended-spectrum beta-lactamase detection",
    abstract = "Objective To compare common extended-spectrum beta-lactamase (ESBL) screening methods and beta-lactams for their ability to detect TEM- and SHV-related ESBL enzymes.Methods This study compared disk diffusion testing by NCCLS methodology, the Jarlier double disk test, a disk-on-disk test, a modified three-dimensional test and the E test method for their sensitivity and specificity in detecting TEM- and SHV-related ESBL producers. Three negative and 22 positive controls were studied. These were two Klebsiella pneumoniae and 23 Escherichia coli transconjugants. Seventeen beta-lactam antibiotics were tested: cefamandole, cefotetan, cefoxitin, cefuroxime, cefixime, cefoperazone, cefotaxime, cefpodoxime, cefsulodin, ceftazidime, ceftibuten, ceftizoxime, ceftriaxone, moxalactam, cefepime, cefpirome and aztreonam.Results NCCLS disk diffusion was 14{\%} sensitive with ceftriaxone, 36{\%} with cefotaxime, 64{\%} with aztreonam, 68{\%} with cefpodoxime, and 73{\%} with ceftazidime. Cefoperazone, cefamandole, cefpodoxime and cefpirome showed 91{\%} sensitivity using the Jarlier test. Using the disk-on-disk test, cefsulodin showed 95{\%} sensitivity, and cefoperazone, cefepime and cefamandole showed 91{\%} sensitivity. With the modified three-dimensional test, cefoperazone, cefpodoxime and cefpirome showed 91{\%} sensitivity.Conclusions For practical reasons, we would recommend use of either the Jarlier test or the commercial cephalosporin disks containing clavulanic acid to screen for ESBL producers. Cefoperazone, cefamandole, cefpodoxime and cefpirome showed good sensitivity across the methods tested.",
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    T1 - Comparison of screening methods for TEM- and SHV-derived extended-spectrum beta-lactamase detection

    AU - MacKenzie, Fiona

    AU - Miller, C. A.

    AU - Gould, Ian M

    PY - 2002

    Y1 - 2002

    N2 - Objective To compare common extended-spectrum beta-lactamase (ESBL) screening methods and beta-lactams for their ability to detect TEM- and SHV-related ESBL enzymes.Methods This study compared disk diffusion testing by NCCLS methodology, the Jarlier double disk test, a disk-on-disk test, a modified three-dimensional test and the E test method for their sensitivity and specificity in detecting TEM- and SHV-related ESBL producers. Three negative and 22 positive controls were studied. These were two Klebsiella pneumoniae and 23 Escherichia coli transconjugants. Seventeen beta-lactam antibiotics were tested: cefamandole, cefotetan, cefoxitin, cefuroxime, cefixime, cefoperazone, cefotaxime, cefpodoxime, cefsulodin, ceftazidime, ceftibuten, ceftizoxime, ceftriaxone, moxalactam, cefepime, cefpirome and aztreonam.Results NCCLS disk diffusion was 14% sensitive with ceftriaxone, 36% with cefotaxime, 64% with aztreonam, 68% with cefpodoxime, and 73% with ceftazidime. Cefoperazone, cefamandole, cefpodoxime and cefpirome showed 91% sensitivity using the Jarlier test. Using the disk-on-disk test, cefsulodin showed 95% sensitivity, and cefoperazone, cefepime and cefamandole showed 91% sensitivity. With the modified three-dimensional test, cefoperazone, cefpodoxime and cefpirome showed 91% sensitivity.Conclusions For practical reasons, we would recommend use of either the Jarlier test or the commercial cephalosporin disks containing clavulanic acid to screen for ESBL producers. Cefoperazone, cefamandole, cefpodoxime and cefpirome showed good sensitivity across the methods tested.

    AB - Objective To compare common extended-spectrum beta-lactamase (ESBL) screening methods and beta-lactams for their ability to detect TEM- and SHV-related ESBL enzymes.Methods This study compared disk diffusion testing by NCCLS methodology, the Jarlier double disk test, a disk-on-disk test, a modified three-dimensional test and the E test method for their sensitivity and specificity in detecting TEM- and SHV-related ESBL producers. Three negative and 22 positive controls were studied. These were two Klebsiella pneumoniae and 23 Escherichia coli transconjugants. Seventeen beta-lactam antibiotics were tested: cefamandole, cefotetan, cefoxitin, cefuroxime, cefixime, cefoperazone, cefotaxime, cefpodoxime, cefsulodin, ceftazidime, ceftibuten, ceftizoxime, ceftriaxone, moxalactam, cefepime, cefpirome and aztreonam.Results NCCLS disk diffusion was 14% sensitive with ceftriaxone, 36% with cefotaxime, 64% with aztreonam, 68% with cefpodoxime, and 73% with ceftazidime. Cefoperazone, cefamandole, cefpodoxime and cefpirome showed 91% sensitivity using the Jarlier test. Using the disk-on-disk test, cefsulodin showed 95% sensitivity, and cefoperazone, cefepime and cefamandole showed 91% sensitivity. With the modified three-dimensional test, cefoperazone, cefpodoxime and cefpirome showed 91% sensitivity.Conclusions For practical reasons, we would recommend use of either the Jarlier test or the commercial cephalosporin disks containing clavulanic acid to screen for ESBL producers. Cefoperazone, cefamandole, cefpodoxime and cefpirome showed good sensitivity across the methods tested.

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    KW - FAMILY ENTEROBACTERIACEAE

    KW - ESCHERICHIA-COLI

    KW - EPIDEMIOLOGY

    KW - PREVALENCE

    KW - OUTBREAK

    KW - MEMBERS

    KW - DISK

    U2 - 10.1046/j.1469-0691.2002.00473.x

    DO - 10.1046/j.1469-0691.2002.00473.x

    M3 - Article

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    JO - Clinical Microbiology and Infection

    JF - Clinical Microbiology and Infection

    SN - 1198-743X

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