Comprehensive phylogenetic diversity of [FeFe]-hydrogenase genes in termite gut microbiota

Hao Zheng, Dylan Bodington, Chong Zhang, Kazuhiko Miyanaga, Yasunori Tanji, Yuichi Hongoh, Xin-Hui Xing

Research output: Contribution to journalArticlepeer-review

Abstract

Phylogenetic diversity of [FeFe]-hydrogenase (HydA) in termite guts was assessed by pyrosequencing PCR amplicons obtained using newly designed primers. Of 8,066 reads, 776 hydA phylotypes, defined with 97% nucleotide sequence identity, were recovered from the gut homogenates of three termite species, Hodotermopsis sjoestedti, Reticulitermes speratus, and Nasutitermes takasagoensis. The phylotype coverage was 92-98%, and the majority shared only low identity with database sequences. It was estimated that 194-745 hydA phylotypes existed in the gut of each termite species. Our results demonstrate that hydA gene diversity in the termite gut microbiota is much higher than previously estimated.

Original languageEnglish
Pages (from-to)491-494
Number of pages4
JournalMicrobes and environments
Volume28
Issue number4
DOIs
Publication statusPublished - 15 Nov 2013

Keywords

  • Animals
  • Bacteria/classification
  • Bacterial Proteins/genetics
  • Biodiversity
  • Gastrointestinal Tract/microbiology
  • Hydrogenase/genetics
  • Iron-Sulfur Proteins/genetics
  • Isoptera/microbiology
  • Microbiota
  • Molecular Sequence Data
  • Phylogeny

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