Concentration and prevalence of Escherichia coli O157 in sheep faeces at pasture in Scotland.

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Abstract

Aims: To study the presence, numbers and virulence profiles of Escherichia coli O157 in sheep faeces and validate the microbiological methods used to attain these data.

Methods and Results: Flock level prevalence was found to be 40% ( six from 15) and 6(.)5% of faecal samples tested were found to be positive. Two farms gave samples defined as high shedding (>10(4) CFU g(-1)), one of which comprised 91% positive samples with 13/33 at the high shedding level.

Conclusions: These data confirmed that sheep are an important reservoir of E. coli O157. Significance and Impact of the Study: Sheep play a significant role in the maintenance and dispersal of E. coli O157 in the farming environment and are an important source of human infection.

Original languageEnglish
Pages (from-to)646-651
Number of pages5
JournalJournal of Applied Microbiology
Volume98
DOIs
Publication statusPublished - 2005

Keywords

  • concentration and prevalence
  • Escherichia coli O157
  • immunomagnetic separation
  • sheep
  • QUANTITATIVE RISK-ASSESSMENT
  • VIRULENCE GENES
  • CATTLE
  • INFECTION
  • OUTBREAK
  • BEEF
  • SEROTYPES
  • SLAUGHTER
  • HAMBURGERS
  • SPAIN

Cite this

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title = "Concentration and prevalence of Escherichia coli O157 in sheep faeces at pasture in Scotland.",
abstract = "Aims: To study the presence, numbers and virulence profiles of Escherichia coli O157 in sheep faeces and validate the microbiological methods used to attain these data.Methods and Results: Flock level prevalence was found to be 40{\%} ( six from 15) and 6(.)5{\%} of faecal samples tested were found to be positive. Two farms gave samples defined as high shedding (>10(4) CFU g(-1)), one of which comprised 91{\%} positive samples with 13/33 at the high shedding level.Conclusions: These data confirmed that sheep are an important reservoir of E. coli O157. Significance and Impact of the Study: Sheep play a significant role in the maintenance and dispersal of E. coli O157 in the farming environment and are an important source of human infection.",
keywords = "concentration and prevalence, Escherichia coli O157, immunomagnetic separation, sheep, QUANTITATIVE RISK-ASSESSMENT, VIRULENCE GENES, CATTLE, INFECTION, OUTBREAK, BEEF, SEROTYPES, SLAUGHTER, HAMBURGERS, SPAIN",
author = "Ogden, {Iain D} and Marion MacRae and Strachan, {Norval James Colin}",
year = "2005",
doi = "10.1111/j.1365-2672.2004.02493.x",
language = "English",
volume = "98",
pages = "646--651",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell",

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TY - JOUR

T1 - Concentration and prevalence of Escherichia coli O157 in sheep faeces at pasture in Scotland.

AU - Ogden, Iain D

AU - MacRae, Marion

AU - Strachan, Norval James Colin

PY - 2005

Y1 - 2005

N2 - Aims: To study the presence, numbers and virulence profiles of Escherichia coli O157 in sheep faeces and validate the microbiological methods used to attain these data.Methods and Results: Flock level prevalence was found to be 40% ( six from 15) and 6(.)5% of faecal samples tested were found to be positive. Two farms gave samples defined as high shedding (>10(4) CFU g(-1)), one of which comprised 91% positive samples with 13/33 at the high shedding level.Conclusions: These data confirmed that sheep are an important reservoir of E. coli O157. Significance and Impact of the Study: Sheep play a significant role in the maintenance and dispersal of E. coli O157 in the farming environment and are an important source of human infection.

AB - Aims: To study the presence, numbers and virulence profiles of Escherichia coli O157 in sheep faeces and validate the microbiological methods used to attain these data.Methods and Results: Flock level prevalence was found to be 40% ( six from 15) and 6(.)5% of faecal samples tested were found to be positive. Two farms gave samples defined as high shedding (>10(4) CFU g(-1)), one of which comprised 91% positive samples with 13/33 at the high shedding level.Conclusions: These data confirmed that sheep are an important reservoir of E. coli O157. Significance and Impact of the Study: Sheep play a significant role in the maintenance and dispersal of E. coli O157 in the farming environment and are an important source of human infection.

KW - concentration and prevalence

KW - Escherichia coli O157

KW - immunomagnetic separation

KW - sheep

KW - QUANTITATIVE RISK-ASSESSMENT

KW - VIRULENCE GENES

KW - CATTLE

KW - INFECTION

KW - OUTBREAK

KW - BEEF

KW - SEROTYPES

KW - SLAUGHTER

KW - HAMBURGERS

KW - SPAIN

U2 - 10.1111/j.1365-2672.2004.02493.x

DO - 10.1111/j.1365-2672.2004.02493.x

M3 - Article

VL - 98

SP - 646

EP - 651

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

ER -