Abstract
The rainbow trout (Oncorhynchus mykiss) is one of the most
extensively researched and characterised species of fish. In addition its low
tolerance to poor water quality has established it as one of the most useful
sentinel species for aquatic toxicology. The subject of proteomics offers a
potentially powerful approach to ecotoxicology, particularly with respect
to providing biomarkers of environmental contamination. Therefore
there is a valid rationale for combining this species with this experimental
approach. However evidence exists that the rainbow trout liver proteome
can be influenced by dietary composition. Furthermore individuals, within a trout population, are known to exhibit widely differing food consumption rates and therefore growth. As a result there may be fundamental issues to consider before this combination can be fully exploited. Using the subject of endocrine disruption preliminary data are presented which demonstrate that individual growth rates moderate the proteomic responses to the injection of a single dose of ß-estradiol. The injection dose is less than required to induce vitellogenin synthesis, the recognised endpoint of endocrine disruption, which suggests these proteomic changes may in fact be more sensitive biomarkers. This study therefore provides evidence and suggested guidelines for proteomic toxicology in rainbow trout. The study also provides evidence that if these considerations are met proteomic changes in the trout liver could be a valuable addition to existing biological markers of aquatic contamination.
extensively researched and characterised species of fish. In addition its low
tolerance to poor water quality has established it as one of the most useful
sentinel species for aquatic toxicology. The subject of proteomics offers a
potentially powerful approach to ecotoxicology, particularly with respect
to providing biomarkers of environmental contamination. Therefore
there is a valid rationale for combining this species with this experimental
approach. However evidence exists that the rainbow trout liver proteome
can be influenced by dietary composition. Furthermore individuals, within a trout population, are known to exhibit widely differing food consumption rates and therefore growth. As a result there may be fundamental issues to consider before this combination can be fully exploited. Using the subject of endocrine disruption preliminary data are presented which demonstrate that individual growth rates moderate the proteomic responses to the injection of a single dose of ß-estradiol. The injection dose is less than required to induce vitellogenin synthesis, the recognised endpoint of endocrine disruption, which suggests these proteomic changes may in fact be more sensitive biomarkers. This study therefore provides evidence and suggested guidelines for proteomic toxicology in rainbow trout. The study also provides evidence that if these considerations are met proteomic changes in the trout liver could be a valuable addition to existing biological markers of aquatic contamination.
| Original language | English |
|---|---|
| Title of host publication | Multiple Stressors |
| Subtitle of host publication | A Challenge for the Future |
| Editors | Carmel Mothersill , Irma Mosse, Colin Seymour |
| Place of Publication | Dordrecht, Netherlands |
| Publisher | Springer |
| Pages | 263-269 |
| Number of pages | 7 |
| ISBN (Electronic) | 978-1-4020-6335-0 |
| ISBN (Print) | 1402063342 , 9781402063343 , 9781402063336 |
| DOIs | |
| Publication status | Published - 17 Aug 2007 |
Publication series
| Name | NATO Science for Peace and Security Series C: Environmental Security |
|---|---|
| Publisher | Springer |
