Considerations for proteomic biomarkers in rainbow trout ecotoxicology

Richard W. Smith, Iurgi Salaberria, Phillip Cash, Peter Part

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The rainbow trout (Oncorhynchus mykiss) is one of the most
extensively researched and characterised species of fish. In addition its low
tolerance to poor water quality has established it as one of the most useful
sentinel species for aquatic toxicology. The subject of proteomics offers a
potentially powerful approach to ecotoxicology, particularly with respect
to providing biomarkers of environmental contamination. Therefore
there is a valid rationale for combining this species with this experimental
approach. However evidence exists that the rainbow trout liver proteome
can be influenced by dietary composition. Furthermore individuals, within a trout population, are known to exhibit widely differing food consumption rates and therefore growth. As a result there may be fundamental issues to consider before this combination can be fully exploited. Using the subject of endocrine disruption preliminary data are presented which demonstrate that individual growth rates moderate the proteomic responses to the injection of a single dose of ß-estradiol. The injection dose is less than required to induce vitellogenin synthesis, the recognised endpoint of endocrine disruption, which suggests these proteomic changes may in fact be more sensitive biomarkers. This study therefore provides evidence and suggested guidelines for proteomic toxicology in rainbow trout. The study also provides evidence that if these considerations are met proteomic changes in the trout liver could be a valuable addition to existing biological markers of aquatic contamination.
Original languageEnglish
Title of host publicationMultiple Stressors
Subtitle of host publicationA Challenge for the Future
EditorsCarmel Mothersill , Irma Mosse, Colin Seymour
Place of PublicationDordrecht, Netherlands
PublisherSpringer
Pages263-269
Number of pages7
ISBN (Electronic)978-1-4020-6335-0
ISBN (Print)1402063342 , 9781402063343 , 9781402063336
DOIs
Publication statusPublished - 17 Aug 2007

Publication series

NameNATO Science for Peace and Security Series C: Environmental Security
PublisherSpringer

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  • Cite this

    Smith, R. W., Salaberria, I., Cash, P., & Part, P. (2007). Considerations for proteomic biomarkers in rainbow trout ecotoxicology. In C. Mothersill , I. Mosse, & C. Seymour (Eds.), Multiple Stressors: A Challenge for the Future (pp. 263-269). (NATO Science for Peace and Security Series C: Environmental Security). Springer . https://doi.org/10.1007/978-1-4020-6335-0