Constitutive and LPS-induced gene expression in a macrophage-like cell line from the rainbow trout (Oncorhynchus mykiss)

J L Brubacher, C J Secombes, J Zou, N C Bols

Research output: Contribution to journalArticle

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Abstract

The lack of cell lines from mononuclear phagocytes of salmonid fish has impeded the study of immune function at a cellular level in these economically and ecologically important animals. Here, we report on the Further characterization of RTS11, a previously described macrophage-like cell line from the rainbow trout spleen, with regard to its expression of a number of immunologically relevant genes. Analysis of gene expression by reverse transcription-polymerase chain reaction, using rainbow-trout-specific primers demonstrated that RTS11 cells express the beta chain of the class II major histocompatibility complex, the cytokines transforming growth factor-beta (TGF-beta) and interleukin-1 beta (IL-1 beta), and cyclo-oxygenase-2 (COX-2). Inducing the cells with lipopolysaccharide led to increased expression of IL-1 beta and COX-2, as determined by Northern blotting. These results together suggest that RTS11 retains many of the characteristics expected of mature macrophages, and should be a valuable tool for further study of the expression and function of these immunomodulatory proteins in fish. (C), 2000 Elsevier Science Ltd, All rights reserved.

Original languageEnglish
Pages (from-to)565-574
Number of pages10
JournalDevelopmental and Comparative Immunology
Volume24
Publication statusPublished - 2000

Keywords

  • rainbow trout
  • macrophage
  • RTS11
  • cytokine
  • IL-1 beta
  • TGF-beta
  • COX-2
  • MHC
  • RESPIRATORY BURST ACTIVITY
  • GROWTH-FACTOR-BETA
  • CARP CYPRINUS-CARPIO
  • SALMO-SALAR L
  • CHANNEL CATFISH
  • PROSTAGLANDIN SYNTHASE
  • ACTIVATING FACTOR(S)
  • TELEOST MACROPHAGES
  • HUMAN NEUTROPHILS
  • MESSENGER-RNA

Cite this

Constitutive and LPS-induced gene expression in a macrophage-like cell line from the rainbow trout (Oncorhynchus mykiss). / Brubacher, J L ; Secombes, C J ; Zou, J ; Bols, N C .

In: Developmental and Comparative Immunology, Vol. 24, 2000, p. 565-574.

Research output: Contribution to journalArticle

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N2 - The lack of cell lines from mononuclear phagocytes of salmonid fish has impeded the study of immune function at a cellular level in these economically and ecologically important animals. Here, we report on the Further characterization of RTS11, a previously described macrophage-like cell line from the rainbow trout spleen, with regard to its expression of a number of immunologically relevant genes. Analysis of gene expression by reverse transcription-polymerase chain reaction, using rainbow-trout-specific primers demonstrated that RTS11 cells express the beta chain of the class II major histocompatibility complex, the cytokines transforming growth factor-beta (TGF-beta) and interleukin-1 beta (IL-1 beta), and cyclo-oxygenase-2 (COX-2). Inducing the cells with lipopolysaccharide led to increased expression of IL-1 beta and COX-2, as determined by Northern blotting. These results together suggest that RTS11 retains many of the characteristics expected of mature macrophages, and should be a valuable tool for further study of the expression and function of these immunomodulatory proteins in fish. (C), 2000 Elsevier Science Ltd, All rights reserved.

AB - The lack of cell lines from mononuclear phagocytes of salmonid fish has impeded the study of immune function at a cellular level in these economically and ecologically important animals. Here, we report on the Further characterization of RTS11, a previously described macrophage-like cell line from the rainbow trout spleen, with regard to its expression of a number of immunologically relevant genes. Analysis of gene expression by reverse transcription-polymerase chain reaction, using rainbow-trout-specific primers demonstrated that RTS11 cells express the beta chain of the class II major histocompatibility complex, the cytokines transforming growth factor-beta (TGF-beta) and interleukin-1 beta (IL-1 beta), and cyclo-oxygenase-2 (COX-2). Inducing the cells with lipopolysaccharide led to increased expression of IL-1 beta and COX-2, as determined by Northern blotting. These results together suggest that RTS11 retains many of the characteristics expected of mature macrophages, and should be a valuable tool for further study of the expression and function of these immunomodulatory proteins in fish. (C), 2000 Elsevier Science Ltd, All rights reserved.

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