Abstract
The lack of cell lines from mononuclear phagocytes of salmonid fish has impeded the study of immune function at a cellular level in these economically and ecologically important animals. Here, we report on the Further characterization of RTS11, a previously described macrophage-like cell line from the rainbow trout spleen, with regard to its expression of a number of immunologically relevant genes. Analysis of gene expression by reverse transcription-polymerase chain reaction, using rainbow-trout-specific primers demonstrated that RTS11 cells express the beta chain of the class II major histocompatibility complex, the cytokines transforming growth factor-beta (TGF-beta) and interleukin-1 beta (IL-1 beta), and cyclo-oxygenase-2 (COX-2). Inducing the cells with lipopolysaccharide led to increased expression of IL-1 beta and COX-2, as determined by Northern blotting. These results together suggest that RTS11 retains many of the characteristics expected of mature macrophages, and should be a valuable tool for further study of the expression and function of these immunomodulatory proteins in fish. (C), 2000 Elsevier Science Ltd, All rights reserved.
Original language | English |
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Pages (from-to) | 565-574 |
Number of pages | 10 |
Journal | Developmental and Comparative Immunology |
Volume | 24 |
Publication status | Published - 2000 |
Keywords
- rainbow trout
- macrophage
- RTS11
- cytokine
- IL-1 beta
- TGF-beta
- COX-2
- MHC
- RESPIRATORY BURST ACTIVITY
- GROWTH-FACTOR-BETA
- CARP CYPRINUS-CARPIO
- SALMO-SALAR L
- CHANNEL CATFISH
- PROSTAGLANDIN SYNTHASE
- ACTIVATING FACTOR(S)
- TELEOST MACROPHAGES
- HUMAN NEUTROPHILS
- MESSENGER-RNA