CONSTRUCTION AND DETECTION OF BIOLUMINESCENT STRAINS OF BACILLUS-SUBTILIS

N COOK, D J SILCOCK, R N WATERHOUSE, James Ivor Prosser, Lesley Anne Glover, Kenneth Stuart Killham

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Abstract

Bioluminescence (lux) genes from Vibrio fischeri and V. harveyi were introduced into Bacillus subtilis on a plasmid vector and by chromosomal integration. The plasmid-bearing strain was highly luminescent and stable under antibiotic selection, but luminescence was lost in the absence of selection and following sporulation and germination. The chromosomally marked strains emitted less light but were found to be stable without the requirement for antibiotic selection and following sporulation and germination. Individual luminescing colonies of both B. subtilis strains could be detected against a high background of non-bioluminescent indigenous soil microbial colonies on agar plates using a charge-coupled device camera. These bioluminescent Gram-positive strains could be of value in studies concerning the survival and spread of genetically-modified micro-organisms in soil environments.

Original languageEnglish
Pages (from-to)350-359
Number of pages10
JournalJournal of Applied Bacteriology
Volume75
Issue number4
Publication statusPublished - Oct 1993

Keywords

  • ESCHERICHIA-COLI
  • SOIL
  • GENE
  • PSEUDOMONAS
  • EXPRESSION
  • CLONING
  • CELL

Cite this

COOK, N., SILCOCK, D. J., WATERHOUSE, R. N., Prosser, J. I., Glover, L. A., & Killham, K. S. (1993). CONSTRUCTION AND DETECTION OF BIOLUMINESCENT STRAINS OF BACILLUS-SUBTILIS. Journal of Applied Bacteriology, 75(4), 350-359.