Cooperative contractility

The role of stress fibres in the regulation of cell-cell junctions

William Ronan*, Robert M. McMeeking, Christopher S. Chen, J. Patrick McGarry, Vikram S. Deshpande

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We present simulations of cell-cell adhesion as reported in a recent study [. Liu et al., 2010, PNAS, 107(22), 9944-9] for two cells seeded on an array of micro-posts. The micro-post array allows for the measurement of forces exerted by the cell and these show that the cell-cell tugging stress is a constant and independent of the cell-cell junction area. In the current study, we demonstrate that a material model which includes the underlying cellular processes of stress fibre contractility and adhesion formation can capture these results. The simulations explain the experimentally observed phenomena whereby the cell-cell junction forces increase with junction size but the tractions exerted by the cell on the micro-post array are independent of the junction size. Further simulations on different types of micro-post arrays and cell phenotypes are presented as a guide to future experiments.

Original languageEnglish
Pages (from-to)520-528
Number of pages9
JournalJournal of Biomechanics
Volume48
Issue number3
Early online date27 Nov 2014
DOIs
Publication statusPublished - 5 Feb 2015

Fingerprint

Stress Fibers
Intercellular Junctions
Cells
Fibers
Cell adhesion
Adhesion
Experiments
Traction
Cell Adhesion
Phenotype

Keywords

  • Cell-cell junctions
  • Junction force
  • Mechanotransduction
  • Myosin contractility
  • Stress fibre

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Rehabilitation
  • Biophysics
  • Biomedical Engineering
  • Medicine(all)

Cite this

Cooperative contractility : The role of stress fibres in the regulation of cell-cell junctions. / Ronan, William; McMeeking, Robert M.; Chen, Christopher S.; McGarry, J. Patrick; Deshpande, Vikram S.

In: Journal of Biomechanics, Vol. 48, No. 3, 05.02.2015, p. 520-528.

Research output: Contribution to journalArticle

Ronan, William ; McMeeking, Robert M. ; Chen, Christopher S. ; McGarry, J. Patrick ; Deshpande, Vikram S. / Cooperative contractility : The role of stress fibres in the regulation of cell-cell junctions. In: Journal of Biomechanics. 2015 ; Vol. 48, No. 3. pp. 520-528.
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