Copper absorption from foods labelled intrinsically and extrinsically with Cu-65 stable isotope

L J Harvey, J R Dainty, John Hamilton Beattie, G Majsak-Newman, S G Wharf, S J Fairweather-Tait

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Objective: To determine copper absorption from copper containing foods labelled either intrinsically or extrinsically with a highly enriched Cu-65 stable isotope label.
    Design: A longitudinal cross-over study.
    Setting: The study was conducted at the Institute of Food Research, Human Nutrition Unit, Norwich, UK.
    Subjects: Subjects were recruited locally via advertisements placed around the Norwich Research Park. A total of 10 volunteers ( nine female, one male) took part in the study, but not all volunteers completed each of the test meals.
    Interventions: A highly enriched Cu-65 stable isotope label was administered to volunteers in the form of a reference dose or in breakfast test meals consisting of red wine, soya beans, mushrooms or sunflower seeds. Faecal monitoring and mass spectrometry techniques were used to estimate the relative quantities of copper absorbed from the different test meals.
    Results: True copper absorption from the reference dose ( 54%) was similar to extrinsically labelled red wine (49%) and intrinsically labelled sunflower seeds (52%), but significantly higher than extrinsically labelled mushrooms (35%), intrinsically (29%) and extrinsically (15%) labelled soya beans and extrinsically labelled sunflower seed (32%) test meals.
    Conclusions: The use of Cu-65 extrinsic labels in copper absorption studies requires validation according to the food being examined; intrinsic and extrinsic labelling produced significantly different results for sunflower seeds.
    Sponsorship: Funding from the European Commission and the Biotechnology and Biological Sciences Research Council.

    Original languageEnglish
    Pages (from-to)363-368
    Number of pages6
    JournalEuropean Journal of Clinical Nutrition
    Volume59
    Issue number3
    Early online date3 Nov 2004
    DOIs
    Publication statusPublished - Mar 2005

    Keywords

    • copper
    • stable isotope
    • absorption
    • adults
    • young men
    • zinc absorption
    • apha cellulose
    • dietary copper
    • bioavailability
    • retention
    • calcium
    • phytate
    • wheat

    Cite this

    Harvey, L. J., Dainty, J. R., Beattie, J. H., Majsak-Newman, G., Wharf, S. G., & Fairweather-Tait, S. J. (2005). Copper absorption from foods labelled intrinsically and extrinsically with Cu-65 stable isotope. European Journal of Clinical Nutrition, 59(3), 363-368. https://doi.org/10.1038/sj.ejcn.1602082

    Copper absorption from foods labelled intrinsically and extrinsically with Cu-65 stable isotope. / Harvey, L J ; Dainty, J R ; Beattie, John Hamilton; Majsak-Newman, G ; Wharf, S G ; Fairweather-Tait, S J .

    In: European Journal of Clinical Nutrition, Vol. 59, No. 3, 03.2005, p. 363-368.

    Research output: Contribution to journalArticle

    Harvey, LJ, Dainty, JR, Beattie, JH, Majsak-Newman, G, Wharf, SG & Fairweather-Tait, SJ 2005, 'Copper absorption from foods labelled intrinsically and extrinsically with Cu-65 stable isotope', European Journal of Clinical Nutrition, vol. 59, no. 3, pp. 363-368. https://doi.org/10.1038/sj.ejcn.1602082
    Harvey, L J ; Dainty, J R ; Beattie, John Hamilton ; Majsak-Newman, G ; Wharf, S G ; Fairweather-Tait, S J . / Copper absorption from foods labelled intrinsically and extrinsically with Cu-65 stable isotope. In: European Journal of Clinical Nutrition. 2005 ; Vol. 59, No. 3. pp. 363-368.
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    abstract = "Objective: To determine copper absorption from copper containing foods labelled either intrinsically or extrinsically with a highly enriched Cu-65 stable isotope label. Design: A longitudinal cross-over study. Setting: The study was conducted at the Institute of Food Research, Human Nutrition Unit, Norwich, UK. Subjects: Subjects were recruited locally via advertisements placed around the Norwich Research Park. A total of 10 volunteers ( nine female, one male) took part in the study, but not all volunteers completed each of the test meals. Interventions: A highly enriched Cu-65 stable isotope label was administered to volunteers in the form of a reference dose or in breakfast test meals consisting of red wine, soya beans, mushrooms or sunflower seeds. Faecal monitoring and mass spectrometry techniques were used to estimate the relative quantities of copper absorbed from the different test meals. Results: True copper absorption from the reference dose ( 54{\%}) was similar to extrinsically labelled red wine (49{\%}) and intrinsically labelled sunflower seeds (52{\%}), but significantly higher than extrinsically labelled mushrooms (35{\%}), intrinsically (29{\%}) and extrinsically (15{\%}) labelled soya beans and extrinsically labelled sunflower seed (32{\%}) test meals. Conclusions: The use of Cu-65 extrinsic labels in copper absorption studies requires validation according to the food being examined; intrinsic and extrinsic labelling produced significantly different results for sunflower seeds. Sponsorship: Funding from the European Commission and the Biotechnology and Biological Sciences Research Council.",
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    AU - Fairweather-Tait, S J

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    N2 - Objective: To determine copper absorption from copper containing foods labelled either intrinsically or extrinsically with a highly enriched Cu-65 stable isotope label. Design: A longitudinal cross-over study. Setting: The study was conducted at the Institute of Food Research, Human Nutrition Unit, Norwich, UK. Subjects: Subjects were recruited locally via advertisements placed around the Norwich Research Park. A total of 10 volunteers ( nine female, one male) took part in the study, but not all volunteers completed each of the test meals. Interventions: A highly enriched Cu-65 stable isotope label was administered to volunteers in the form of a reference dose or in breakfast test meals consisting of red wine, soya beans, mushrooms or sunflower seeds. Faecal monitoring and mass spectrometry techniques were used to estimate the relative quantities of copper absorbed from the different test meals. Results: True copper absorption from the reference dose ( 54%) was similar to extrinsically labelled red wine (49%) and intrinsically labelled sunflower seeds (52%), but significantly higher than extrinsically labelled mushrooms (35%), intrinsically (29%) and extrinsically (15%) labelled soya beans and extrinsically labelled sunflower seed (32%) test meals. Conclusions: The use of Cu-65 extrinsic labels in copper absorption studies requires validation according to the food being examined; intrinsic and extrinsic labelling produced significantly different results for sunflower seeds. Sponsorship: Funding from the European Commission and the Biotechnology and Biological Sciences Research Council.

    AB - Objective: To determine copper absorption from copper containing foods labelled either intrinsically or extrinsically with a highly enriched Cu-65 stable isotope label. Design: A longitudinal cross-over study. Setting: The study was conducted at the Institute of Food Research, Human Nutrition Unit, Norwich, UK. Subjects: Subjects were recruited locally via advertisements placed around the Norwich Research Park. A total of 10 volunteers ( nine female, one male) took part in the study, but not all volunteers completed each of the test meals. Interventions: A highly enriched Cu-65 stable isotope label was administered to volunteers in the form of a reference dose or in breakfast test meals consisting of red wine, soya beans, mushrooms or sunflower seeds. Faecal monitoring and mass spectrometry techniques were used to estimate the relative quantities of copper absorbed from the different test meals. Results: True copper absorption from the reference dose ( 54%) was similar to extrinsically labelled red wine (49%) and intrinsically labelled sunflower seeds (52%), but significantly higher than extrinsically labelled mushrooms (35%), intrinsically (29%) and extrinsically (15%) labelled soya beans and extrinsically labelled sunflower seed (32%) test meals. Conclusions: The use of Cu-65 extrinsic labels in copper absorption studies requires validation according to the food being examined; intrinsic and extrinsic labelling produced significantly different results for sunflower seeds. Sponsorship: Funding from the European Commission and the Biotechnology and Biological Sciences Research Council.

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    KW - young men

    KW - zinc absorption

    KW - apha cellulose

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    KW - retention

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    KW - phytate

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