Copper speciation and impacts on bacterial biosensors in the pore water of copper-contaminated soils

R Vulkan, F J Zhao, V Barbosa-Jefferson, S Preston, G I Paton, E Tipping, S P McGrath

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Knowledge of heavy metal speciation and its relationship with biological responses is important for the derivation bf effects-based soil quality criteria. We determined soluble Cu concentrations and free Cu2+ activities in the pore waters from 22 soils with total Cu varying from 19 to 8645 mg kg(-1). Pore water pCu(2+), (= -log (Cu2+ activity)) varied from 3.9 to 10.5 and was controlled by soil pH and total Cu concentration. The percentage of free Cu2+ in total soluble Cu varied from 0.02 to 96% and was influenced strongly by pore water pH and, to a lesser extent, by dissolved organic C. In the pore waters with pH >6, the percentage of free Cu2+ in total soluble Cu was lo;ver than 1%. Using the default data base and with the fulvic acid content of DOC optimized at 69%, the equilibrium speciation program WHAM/Model VI gave estimates of pCu(2+) that agreed closely with measured values. Pore water samples were analyzed by two bioluminescence-based bacterial biosensors: Escherichia coli HB101 pUCD607 and Pseudomonas fluorescens 10586r pUCD607. The response of P. fluorescens correlated more closely with soil pore water pCu(2+) than with soluble Cu concentration, whereas pCu(2+) and soluble Cu fitted the response of E. coli equally well. The effect concentrations (EC25 and EC50 values) of pCu(2+) for the two biosensors were about 5.8 and 5.0, respectively. This is the first time that threshold values for Cu have been obtained for bacterial biosensors exposed to soil pore water from well-equilibrated contaminated soils.

Original languageEnglish
Pages (from-to)5115-5121
Number of pages7
JournalEnvironmental Science & Technology
Volume34
DOIs
Publication statusPublished - 2000

Keywords

  • CHEMICAL SPECIATION
  • SEWAGE-SLUDGE
  • HEAVY-METALS
  • TOXICITY
  • BIOAVAILABILITY
  • ZN
  • COMPLEXATION
  • ZINC
  • ALGA
  • CD

Cite this

Copper speciation and impacts on bacterial biosensors in the pore water of copper-contaminated soils. / Vulkan, R ; Zhao, F J ; Barbosa-Jefferson, V ; Preston, S ; Paton, G I ; Tipping, E ; McGrath, S P .

In: Environmental Science & Technology, Vol. 34, 2000, p. 5115-5121.

Research output: Contribution to journalArticle

Vulkan, R ; Zhao, F J ; Barbosa-Jefferson, V ; Preston, S ; Paton, G I ; Tipping, E ; McGrath, S P . / Copper speciation and impacts on bacterial biosensors in the pore water of copper-contaminated soils. In: Environmental Science & Technology. 2000 ; Vol. 34. pp. 5115-5121.
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abstract = "Knowledge of heavy metal speciation and its relationship with biological responses is important for the derivation bf effects-based soil quality criteria. We determined soluble Cu concentrations and free Cu2+ activities in the pore waters from 22 soils with total Cu varying from 19 to 8645 mg kg(-1). Pore water pCu(2+), (= -log (Cu2+ activity)) varied from 3.9 to 10.5 and was controlled by soil pH and total Cu concentration. The percentage of free Cu2+ in total soluble Cu varied from 0.02 to 96{\%} and was influenced strongly by pore water pH and, to a lesser extent, by dissolved organic C. In the pore waters with pH >6, the percentage of free Cu2+ in total soluble Cu was lo;ver than 1{\%}. Using the default data base and with the fulvic acid content of DOC optimized at 69{\%}, the equilibrium speciation program WHAM/Model VI gave estimates of pCu(2+) that agreed closely with measured values. Pore water samples were analyzed by two bioluminescence-based bacterial biosensors: Escherichia coli HB101 pUCD607 and Pseudomonas fluorescens 10586r pUCD607. The response of P. fluorescens correlated more closely with soil pore water pCu(2+) than with soluble Cu concentration, whereas pCu(2+) and soluble Cu fitted the response of E. coli equally well. The effect concentrations (EC25 and EC50 values) of pCu(2+) for the two biosensors were about 5.8 and 5.0, respectively. This is the first time that threshold values for Cu have been obtained for bacterial biosensors exposed to soil pore water from well-equilibrated contaminated soils.",
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T1 - Copper speciation and impacts on bacterial biosensors in the pore water of copper-contaminated soils

AU - Vulkan, R

AU - Zhao, F J

AU - Barbosa-Jefferson, V

AU - Preston, S

AU - Paton, G I

AU - Tipping, E

AU - McGrath, S P

PY - 2000

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N2 - Knowledge of heavy metal speciation and its relationship with biological responses is important for the derivation bf effects-based soil quality criteria. We determined soluble Cu concentrations and free Cu2+ activities in the pore waters from 22 soils with total Cu varying from 19 to 8645 mg kg(-1). Pore water pCu(2+), (= -log (Cu2+ activity)) varied from 3.9 to 10.5 and was controlled by soil pH and total Cu concentration. The percentage of free Cu2+ in total soluble Cu varied from 0.02 to 96% and was influenced strongly by pore water pH and, to a lesser extent, by dissolved organic C. In the pore waters with pH >6, the percentage of free Cu2+ in total soluble Cu was lo;ver than 1%. Using the default data base and with the fulvic acid content of DOC optimized at 69%, the equilibrium speciation program WHAM/Model VI gave estimates of pCu(2+) that agreed closely with measured values. Pore water samples were analyzed by two bioluminescence-based bacterial biosensors: Escherichia coli HB101 pUCD607 and Pseudomonas fluorescens 10586r pUCD607. The response of P. fluorescens correlated more closely with soil pore water pCu(2+) than with soluble Cu concentration, whereas pCu(2+) and soluble Cu fitted the response of E. coli equally well. The effect concentrations (EC25 and EC50 values) of pCu(2+) for the two biosensors were about 5.8 and 5.0, respectively. This is the first time that threshold values for Cu have been obtained for bacterial biosensors exposed to soil pore water from well-equilibrated contaminated soils.

AB - Knowledge of heavy metal speciation and its relationship with biological responses is important for the derivation bf effects-based soil quality criteria. We determined soluble Cu concentrations and free Cu2+ activities in the pore waters from 22 soils with total Cu varying from 19 to 8645 mg kg(-1). Pore water pCu(2+), (= -log (Cu2+ activity)) varied from 3.9 to 10.5 and was controlled by soil pH and total Cu concentration. The percentage of free Cu2+ in total soluble Cu varied from 0.02 to 96% and was influenced strongly by pore water pH and, to a lesser extent, by dissolved organic C. In the pore waters with pH >6, the percentage of free Cu2+ in total soluble Cu was lo;ver than 1%. Using the default data base and with the fulvic acid content of DOC optimized at 69%, the equilibrium speciation program WHAM/Model VI gave estimates of pCu(2+) that agreed closely with measured values. Pore water samples were analyzed by two bioluminescence-based bacterial biosensors: Escherichia coli HB101 pUCD607 and Pseudomonas fluorescens 10586r pUCD607. The response of P. fluorescens correlated more closely with soil pore water pCu(2+) than with soluble Cu concentration, whereas pCu(2+) and soluble Cu fitted the response of E. coli equally well. The effect concentrations (EC25 and EC50 values) of pCu(2+) for the two biosensors were about 5.8 and 5.0, respectively. This is the first time that threshold values for Cu have been obtained for bacterial biosensors exposed to soil pore water from well-equilibrated contaminated soils.

KW - CHEMICAL SPECIATION

KW - SEWAGE-SLUDGE

KW - HEAVY-METALS

KW - TOXICITY

KW - BIOAVAILABILITY

KW - ZN

KW - COMPLEXATION

KW - ZINC

KW - ALGA

KW - CD

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