C.P5 Tumour necrosis factor alpha and rainbow trout (Oncorhynchus mykiss) leucocyte responses

G. Daniels; S. I. Jang; L. J. Hardie; C. J. Secombes

doi:10.1016/0145-305X(94)90089-2

C.P5 Tumour necrosis factor alpha and rainbow trout (Oncorhynchus mykiss) leucocyte responses

G. Daniels^*, S. I. Jang, L. J. Hardie, C. J. Secombes

^*Corresponding author for this work

Aberdeen Centre For Environmental Sustainability

Research output: Contribution to journal › Article › peer-review

Abstract

Earlier studies have shown that rainbow trout lymphocytes and macrophages can respond to recombinant
human tumour necrosis factor alpha (hTNFc 0. Such studies suggest that a TNFc~ receptor exists on these
cells. To test this, a battery of 6 monoclonal antibodies (MoAb) to the 55 kDa TNF receptor (TNFR-1) were
used to attempt inhibition of macrophage responsiveness to hTNFt~, and two (5R2, 5R16) were successful.
These MoAb also inhibited, in part, the increase in macrophage respiratory burst activity seen after
incubation with a trout macrophage activating factor-containing supernatant. In addition, supernatants from
macrophages stimulated with MAF-supernatants, LPS and TNF~ were found to increase macrophage
respiratory burst activity, and this activity could be inhibited with 5R2 and 5R16. Such findings suggest that
fish leucocytes are able to release a TNFc~-like molecule. However, parallel molecular studies on TNFct,
using primers to conserved regions of the mammalian gene, have to date failed to hybridise to trout
splenocyte or macrophage cDNA.

Original language	English
Journal	Developmental and Comparative Immunology
Volume	18
Issue number	SUPPL. 1
DOIs	https://doi.org/10.1016/0145-305X(94)90089-2
Publication status	Published - 1 Dec 1994

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@article{38b73236b1b347df81258a437ea9d057,

title = "C.P5 Tumour necrosis factor alpha and rainbow trout (Oncorhynchus mykiss) leucocyte responses",

abstract = "Earlier studies have shown that rainbow trout lymphocytes and macrophages can respond to recombinant human tumour necrosis factor alpha (hTNFc 0. Such studies suggest that a TNFc~ receptor exists on these cells. To test this, a battery of 6 monoclonal antibodies (MoAb) to the 55 kDa TNF receptor (TNFR-1) were used to attempt inhibition of macrophage responsiveness to hTNFt~, and two (5R2, 5R16) were successful. These MoAb also inhibited, in part, the increase in macrophage respiratory burst activity seen after incubation with a trout macrophage activating factor-containing supernatant. In addition, supernatants from macrophages stimulated with MAF-supernatants, LPS and TNF~ were found to increase macrophage respiratory burst activity, and this activity could be inhibited with 5R2 and 5R16. Such findings suggest that fish leucocytes are able to release a TNFc~-like molecule. However, parallel molecular studies on TNFct, using primers to conserved regions of the mammalian gene, have to date failed to hybridise to trout splenocyte or macrophage cDNA. ",

author = "G. Daniels and Jang, {S. I.} and Hardie, {L. J.} and Secombes, {C. J.}",

year = "1994",

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AU - Daniels, G.

AU - Jang, S. I.

AU - Hardie, L. J.

AU - Secombes, C. J.

PY - 1994/12/1

Y1 - 1994/12/1

N2 - Earlier studies have shown that rainbow trout lymphocytes and macrophages can respond to recombinant human tumour necrosis factor alpha (hTNFc 0. Such studies suggest that a TNFc~ receptor exists on these cells. To test this, a battery of 6 monoclonal antibodies (MoAb) to the 55 kDa TNF receptor (TNFR-1) were used to attempt inhibition of macrophage responsiveness to hTNFt~, and two (5R2, 5R16) were successful. These MoAb also inhibited, in part, the increase in macrophage respiratory burst activity seen after incubation with a trout macrophage activating factor-containing supernatant. In addition, supernatants from macrophages stimulated with MAF-supernatants, LPS and TNF~ were found to increase macrophage respiratory burst activity, and this activity could be inhibited with 5R2 and 5R16. Such findings suggest that fish leucocytes are able to release a TNFc~-like molecule. However, parallel molecular studies on TNFct, using primers to conserved regions of the mammalian gene, have to date failed to hybridise to trout splenocyte or macrophage cDNA.

AB - Earlier studies have shown that rainbow trout lymphocytes and macrophages can respond to recombinant human tumour necrosis factor alpha (hTNFc 0. Such studies suggest that a TNFc~ receptor exists on these cells. To test this, a battery of 6 monoclonal antibodies (MoAb) to the 55 kDa TNF receptor (TNFR-1) were used to attempt inhibition of macrophage responsiveness to hTNFt~, and two (5R2, 5R16) were successful. These MoAb also inhibited, in part, the increase in macrophage respiratory burst activity seen after incubation with a trout macrophage activating factor-containing supernatant. In addition, supernatants from macrophages stimulated with MAF-supernatants, LPS and TNF~ were found to increase macrophage respiratory burst activity, and this activity could be inhibited with 5R2 and 5R16. Such findings suggest that fish leucocytes are able to release a TNFc~-like molecule. However, parallel molecular studies on TNFct, using primers to conserved regions of the mammalian gene, have to date failed to hybridise to trout splenocyte or macrophage cDNA.

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C.P5 Tumour necrosis factor alpha and rainbow trout (Oncorhynchus mykiss) leucocyte responses

Abstract

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