CpG oligodeoxynucleotides stimulate immune cell proliferation but not specific antibody production in rainbow trout (Oncorhynchus mykiss)

Allison Christina Carrington, Bertrand Collet, Jason William Holland, Christopher John Secombes

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Bacterial DNA and CpG ODN have both been shown to have immunostimulatory effects in mammals, activating APCs and inducing a potent Th1 type immune response. They have also been shown to have a strong adjuvant effect and up-regulate MHC class 2 expression in murine cells, augment human and murine NK cell lytic activity, activate human B cells and induce murine B cell proliferation. However, little work has been carried out with regard to their effects on the piscine immune system. Here it is shown that various CpG ODN induce proliferation of peripheral blood leucocytes, spleen and head kidney cells from rainbow trout although, at the range of concentrations tested CpG ODN 2133 lacked the ability to induce specific antibody production to a protein antigen. (C) 2004 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)211-222
Number of pages11
JournalVeterinary Immunology and Immunopathology
Volume101
Issue number3-4
DOIs
Publication statusPublished - 2004

Keywords

  • CpG
  • proliferation
  • antibody production
  • rainbow trout
  • MURINE B-CELLS
  • BACTERIAL-DNA
  • IN-VITRO
  • SYNTHETIC OLIGODEOXYNUCLEOTIDES
  • T-CELLS
  • MOTIFS
  • ACTIVATION
  • IMMUNOGENICITY
  • CHLOROQUINE
  • MACROPHAGES

Cite this

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title = "CpG oligodeoxynucleotides stimulate immune cell proliferation but not specific antibody production in rainbow trout (Oncorhynchus mykiss)",
abstract = "Bacterial DNA and CpG ODN have both been shown to have immunostimulatory effects in mammals, activating APCs and inducing a potent Th1 type immune response. They have also been shown to have a strong adjuvant effect and up-regulate MHC class 2 expression in murine cells, augment human and murine NK cell lytic activity, activate human B cells and induce murine B cell proliferation. However, little work has been carried out with regard to their effects on the piscine immune system. Here it is shown that various CpG ODN induce proliferation of peripheral blood leucocytes, spleen and head kidney cells from rainbow trout although, at the range of concentrations tested CpG ODN 2133 lacked the ability to induce specific antibody production to a protein antigen. (C) 2004 Elsevier B.V. All rights reserved.",
keywords = "CpG, proliferation, antibody production, rainbow trout, MURINE B-CELLS, BACTERIAL-DNA, IN-VITRO, SYNTHETIC OLIGODEOXYNUCLEOTIDES, T-CELLS, MOTIFS, ACTIVATION, IMMUNOGENICITY, CHLOROQUINE, MACROPHAGES",
author = "Carrington, {Allison Christina} and Bertrand Collet and Holland, {Jason William} and Secombes, {Christopher John}",
year = "2004",
doi = "10.1016/j.vetimm.2004.04.022",
language = "English",
volume = "101",
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journal = "Veterinary Immunology and Immunopathology",
issn = "0165-2427",
publisher = "Elsevier",
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TY - JOUR

T1 - CpG oligodeoxynucleotides stimulate immune cell proliferation but not specific antibody production in rainbow trout (Oncorhynchus mykiss)

AU - Carrington, Allison Christina

AU - Collet, Bertrand

AU - Holland, Jason William

AU - Secombes, Christopher John

PY - 2004

Y1 - 2004

N2 - Bacterial DNA and CpG ODN have both been shown to have immunostimulatory effects in mammals, activating APCs and inducing a potent Th1 type immune response. They have also been shown to have a strong adjuvant effect and up-regulate MHC class 2 expression in murine cells, augment human and murine NK cell lytic activity, activate human B cells and induce murine B cell proliferation. However, little work has been carried out with regard to their effects on the piscine immune system. Here it is shown that various CpG ODN induce proliferation of peripheral blood leucocytes, spleen and head kidney cells from rainbow trout although, at the range of concentrations tested CpG ODN 2133 lacked the ability to induce specific antibody production to a protein antigen. (C) 2004 Elsevier B.V. All rights reserved.

AB - Bacterial DNA and CpG ODN have both been shown to have immunostimulatory effects in mammals, activating APCs and inducing a potent Th1 type immune response. They have also been shown to have a strong adjuvant effect and up-regulate MHC class 2 expression in murine cells, augment human and murine NK cell lytic activity, activate human B cells and induce murine B cell proliferation. However, little work has been carried out with regard to their effects on the piscine immune system. Here it is shown that various CpG ODN induce proliferation of peripheral blood leucocytes, spleen and head kidney cells from rainbow trout although, at the range of concentrations tested CpG ODN 2133 lacked the ability to induce specific antibody production to a protein antigen. (C) 2004 Elsevier B.V. All rights reserved.

KW - CpG

KW - proliferation

KW - antibody production

KW - rainbow trout

KW - MURINE B-CELLS

KW - BACTERIAL-DNA

KW - IN-VITRO

KW - SYNTHETIC OLIGODEOXYNUCLEOTIDES

KW - T-CELLS

KW - MOTIFS

KW - ACTIVATION

KW - IMMUNOGENICITY

KW - CHLOROQUINE

KW - MACROPHAGES

U2 - 10.1016/j.vetimm.2004.04.022

DO - 10.1016/j.vetimm.2004.04.022

M3 - Article

VL - 101

SP - 211

EP - 222

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

SN - 0165-2427

IS - 3-4

ER -