Cross-bridge apparent rate constants of human gallbladder smooth muscles

Wenguang Li, Xiaoyu Luo, Tianhai Tian, Nicholas Hill, Raymond William Ogden, Anne Smythe, Ali Majeed, Nigel Bird

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This paper studies human gallbladder (GB) smooth muscle contractions. A two-state cross-bridge model was used to estimate the apparent attachment and detachment rate constants, as well as increased Ca2+ concentration from the peak active stress during the isometric contraction. The active stress was computed from a mechanical model based entirely on non-invasive routine ultrasound scans. In the two-state cross-bridge model, the two apparent rate constants, representing the total attached/detached cross-bridges, respectively, were estimated using active stress prediction for 51 subjects undergoing cholecystokinin-provocation test, together with estimates from the four-state cross-bridge model for a swine carotid, bovine tracheal and guinea pig GB smooth muscles. The study suggests that the apparent rate constants should be patient-specific, i.e. patients with a lower stress level are characterized by smaller apparent rate constants. In other words, the diseased GB may need to develop fast cycling cross-bridges to compensate in the emptying process. This is a first step towards more quantitative and non-invasive measures of GB pain, and may provide useful insight in understanding GB motility and developing effective drug treatments.
Original languageEnglish
Pages (from-to)209-220
Number of pages12
JournalJournal of Muscle Research and Cell Motility
Volume32
Issue number3
Early online date27 Sep 2011
DOIs
Publication statusPublished - 2011

Fingerprint

Gallbladder
Smooth Muscle
Muscle
Rate constants
Gallbladder Diseases
Isometric Contraction
Cholecystokinin
Drug therapy
Muscle Contraction
Guinea Pigs
Swine
Pain
Ultrasonics
Pharmaceutical Preparations
Therapeutics

Keywords

  • Gallbladder
  • Active stress
  • CCK
  • Isometric contraction
  • Acalculous biliary pain
  • Cross-bridge
  • Apparent rate constant
  • Ca2+

Cite this

Cross-bridge apparent rate constants of human gallbladder smooth muscles. / Li, Wenguang; Luo, Xiaoyu; Tian, Tianhai; Hill, Nicholas; Ogden, Raymond William; Smythe, Anne; Majeed, Ali; Bird, Nigel.

In: Journal of Muscle Research and Cell Motility, Vol. 32, No. 3, 2011, p. 209-220.

Research output: Contribution to journalArticle

Li, W, Luo, X, Tian, T, Hill, N, Ogden, RW, Smythe, A, Majeed, A & Bird, N 2011, 'Cross-bridge apparent rate constants of human gallbladder smooth muscles', Journal of Muscle Research and Cell Motility, vol. 32, no. 3, pp. 209-220. https://doi.org/10.1007/s10974-011-9260-y
Li, Wenguang ; Luo, Xiaoyu ; Tian, Tianhai ; Hill, Nicholas ; Ogden, Raymond William ; Smythe, Anne ; Majeed, Ali ; Bird, Nigel. / Cross-bridge apparent rate constants of human gallbladder smooth muscles. In: Journal of Muscle Research and Cell Motility. 2011 ; Vol. 32, No. 3. pp. 209-220.
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AU - Luo, Xiaoyu

AU - Tian, Tianhai

AU - Hill, Nicholas

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AU - Smythe, Anne

AU - Majeed, Ali

AU - Bird, Nigel

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N2 - This paper studies human gallbladder (GB) smooth muscle contractions. A two-state cross-bridge model was used to estimate the apparent attachment and detachment rate constants, as well as increased Ca2+ concentration from the peak active stress during the isometric contraction. The active stress was computed from a mechanical model based entirely on non-invasive routine ultrasound scans. In the two-state cross-bridge model, the two apparent rate constants, representing the total attached/detached cross-bridges, respectively, were estimated using active stress prediction for 51 subjects undergoing cholecystokinin-provocation test, together with estimates from the four-state cross-bridge model for a swine carotid, bovine tracheal and guinea pig GB smooth muscles. The study suggests that the apparent rate constants should be patient-specific, i.e. patients with a lower stress level are characterized by smaller apparent rate constants. In other words, the diseased GB may need to develop fast cycling cross-bridges to compensate in the emptying process. This is a first step towards more quantitative and non-invasive measures of GB pain, and may provide useful insight in understanding GB motility and developing effective drug treatments.

AB - This paper studies human gallbladder (GB) smooth muscle contractions. A two-state cross-bridge model was used to estimate the apparent attachment and detachment rate constants, as well as increased Ca2+ concentration from the peak active stress during the isometric contraction. The active stress was computed from a mechanical model based entirely on non-invasive routine ultrasound scans. In the two-state cross-bridge model, the two apparent rate constants, representing the total attached/detached cross-bridges, respectively, were estimated using active stress prediction for 51 subjects undergoing cholecystokinin-provocation test, together with estimates from the four-state cross-bridge model for a swine carotid, bovine tracheal and guinea pig GB smooth muscles. The study suggests that the apparent rate constants should be patient-specific, i.e. patients with a lower stress level are characterized by smaller apparent rate constants. In other words, the diseased GB may need to develop fast cycling cross-bridges to compensate in the emptying process. This is a first step towards more quantitative and non-invasive measures of GB pain, and may provide useful insight in understanding GB motility and developing effective drug treatments.

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