Crystal structure of a shark single-domain antibody V region in complex with lysozyme

Robyn L Stanfield, Helen Dooley, Martin F Flajnik, Ian A Wilson

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

Cartilaginous fish are the phylogenetically oldest living organisms known to possess components of the vertebrate adaptive immune system. Key to their immune response are heavy-chain, homodimeric immunoglobulins called new antigen receptors (IgNARs), in which the variable (V) domains recognize antigens with only a single immunoglobulin domain, akin to camelid heavy-chain V domains. The 1.45 angstrom resolution crystal structure of the type I IgNAR V domain in complex with hen egg-white lysozyme (HEL) reveals a minimal antigen-binding domain that contains only two of the three conventional complementarity-determining regions but still binds HEL with nanomolar affinity by means of a binding interface comparable in size to conventional antibodies.
Original languageEnglish
Pages (from-to)1770-3
Number of pages4
JournalScience
Volume305
Issue number5691
DOIs
Publication statusPublished - 2004

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Single-Domain Antibodies
Sharks
Egg White
Antigen Receptors
Muramidase
Complementarity Determining Regions
Antigens
Immunoglobulin Heavy Chains
Vertebrates
Immunoglobulins
Immune System
Fishes
Antibodies
hen egg lysozyme

Cite this

Stanfield, R. L., Dooley, H., Flajnik, M. F., & Wilson, I. A. (2004). Crystal structure of a shark single-domain antibody V region in complex with lysozyme. Science, 305(5691), 1770-3. https://doi.org/10.1126/science.1101148

Crystal structure of a shark single-domain antibody V region in complex with lysozyme. / Stanfield, Robyn L; Dooley, Helen; Flajnik, Martin F; Wilson, Ian A.

In: Science, Vol. 305, No. 5691, 2004, p. 1770-3.

Research output: Contribution to journalArticle

Stanfield, RL, Dooley, H, Flajnik, MF & Wilson, IA 2004, 'Crystal structure of a shark single-domain antibody V region in complex with lysozyme', Science, vol. 305, no. 5691, pp. 1770-3. https://doi.org/10.1126/science.1101148
Stanfield, Robyn L ; Dooley, Helen ; Flajnik, Martin F ; Wilson, Ian A. / Crystal structure of a shark single-domain antibody V region in complex with lysozyme. In: Science. 2004 ; Vol. 305, No. 5691. pp. 1770-3.
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