Crystal structure of a shark single-domain antibody V region in complex with lysozyme

Robyn L Stanfield, Helen Dooley, Martin F Flajnik, Ian A Wilson

Research output: Contribution to journalArticlepeer-review

191 Citations (Scopus)

Abstract

Cartilaginous fish are the phylogenetically oldest living organisms known to possess components of the vertebrate adaptive immune system. Key to their immune response are heavy-chain, homodimeric immunoglobulins called new antigen receptors (IgNARs), in which the variable (V) domains recognize antigens with only a single immunoglobulin domain, akin to camelid heavy-chain V domains. The 1.45 angstrom resolution crystal structure of the type I IgNAR V domain in complex with hen egg-white lysozyme (HEL) reveals a minimal antigen-binding domain that contains only two of the three conventional complementarity-determining regions but still binds HEL with nanomolar affinity by means of a binding interface comparable in size to conventional antibodies.
Original languageEnglish
Pages (from-to)1770-3
Number of pages4
JournalScience
Volume305
Issue number5691
DOIs
Publication statusPublished - 2004

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