CtBP3/BARS drives membrane fission in dynamin-independent transport pathways

Matteo Bonazzi; Stefania Spanò; Gabriele Turacchio; Claudia Cericola; Carmen Valente; Antonino Colanzi; Hee Seok Kweon; Victor W Hsu; Elena V Polishchuck; Roman S Polishchuck; Michele Sallese; Teodoro Pulvirenti; Daniela Corda; Alberto Luini

doi:10.1038/ncb1260

CtBP3/BARS drives membrane fission in dynamin-independent transport pathways

Matteo Bonazzi, Stefania Spanò, Gabriele Turacchio, Claudia Cericola, Carmen Valente, Antonino Colanzi, Hee Seok Kweon, Victor W Hsu, Elena V Polishchuck, Roman S Polishchuck, Michele Sallese, Teodoro Pulvirenti, Daniela Corda, Alberto Luini

Medical Sciences

Research output: Contribution to journal › Article › peer-review

151 Citations (Scopus)

Abstract

Membrane fission is a fundamental step in membrane transport. So far, the only fission protein machinery that has been implicated in in vivo transport involves dynamin, and functions in several, but not all, transport pathways. Thus, other fission machineries may exist. Here, we report that carboxy-terminal binding protein 3/brefeldin A-ribosylated substrate (CtBP3/BARS) controls fission in basolateral transport from the Golgi to the plasma membrane and in fluid-phase endocytosis, whereas dynamin is not involved in these steps. Conversely, CtBP3/BARS protein is inactive in apical transport to the plasma membrane and in receptor-mediated endocytosis, both steps being controlled by dynamin. This indicates that CtBP3/BARS controls membrane fission in endocytic and exocytic transport pathways, distinct from those that require dynamin.

Original language	English
Pages (from-to)	570-80
Number of pages	11
Journal	Nature Cell Biology
Volume	7
Issue number	6
DOIs	https://doi.org/10.1038/ncb1260
Publication status	Published - Jun 2005

Keywords

Animals
COS Cells
Carrier Proteins
Cell Membrane
Cercopithecus aethiops
Dogs
Dynamins
Endocytosis
Exocytosis
Golgi Apparatus
Intracellular Membranes
Microscopy, Electron, Transmission
Organelles
Protein Transport
Receptors, Cell Surface
Transcription Factors
Transport Vesicles

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title = "CtBP3/BARS drives membrane fission in dynamin-independent transport pathways",

abstract = "Membrane fission is a fundamental step in membrane transport. So far, the only fission protein machinery that has been implicated in in vivo transport involves dynamin, and functions in several, but not all, transport pathways. Thus, other fission machineries may exist. Here, we report that carboxy-terminal binding protein 3/brefeldin A-ribosylated substrate (CtBP3/BARS) controls fission in basolateral transport from the Golgi to the plasma membrane and in fluid-phase endocytosis, whereas dynamin is not involved in these steps. Conversely, CtBP3/BARS protein is inactive in apical transport to the plasma membrane and in receptor-mediated endocytosis, both steps being controlled by dynamin. This indicates that CtBP3/BARS controls membrane fission in endocytic and exocytic transport pathways, distinct from those that require dynamin.",

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author = "Matteo Bonazzi and Stefania Span{\`o} and Gabriele Turacchio and Claudia Cericola and Carmen Valente and Antonino Colanzi and Kweon, {Hee Seok} and Hsu, {Victor W} and Polishchuck, {Elena V} and Polishchuck, {Roman S} and Michele Sallese and Teodoro Pulvirenti and Daniela Corda and Alberto Luini",

year = "2005",

month = jun,

doi = "10.1038/ncb1260",

language = "English",

volume = "7",

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journal = "Nature Cell Biology",

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T1 - CtBP3/BARS drives membrane fission in dynamin-independent transport pathways

AU - Bonazzi, Matteo

AU - Spanò, Stefania

AU - Turacchio, Gabriele

AU - Cericola, Claudia

AU - Valente, Carmen

AU - Colanzi, Antonino

AU - Kweon, Hee Seok

AU - Hsu, Victor W

AU - Polishchuck, Elena V

AU - Polishchuck, Roman S

AU - Sallese, Michele

AU - Pulvirenti, Teodoro

AU - Corda, Daniela

AU - Luini, Alberto

PY - 2005/6

Y1 - 2005/6

N2 - Membrane fission is a fundamental step in membrane transport. So far, the only fission protein machinery that has been implicated in in vivo transport involves dynamin, and functions in several, but not all, transport pathways. Thus, other fission machineries may exist. Here, we report that carboxy-terminal binding protein 3/brefeldin A-ribosylated substrate (CtBP3/BARS) controls fission in basolateral transport from the Golgi to the plasma membrane and in fluid-phase endocytosis, whereas dynamin is not involved in these steps. Conversely, CtBP3/BARS protein is inactive in apical transport to the plasma membrane and in receptor-mediated endocytosis, both steps being controlled by dynamin. This indicates that CtBP3/BARS controls membrane fission in endocytic and exocytic transport pathways, distinct from those that require dynamin.

AB - Membrane fission is a fundamental step in membrane transport. So far, the only fission protein machinery that has been implicated in in vivo transport involves dynamin, and functions in several, but not all, transport pathways. Thus, other fission machineries may exist. Here, we report that carboxy-terminal binding protein 3/brefeldin A-ribosylated substrate (CtBP3/BARS) controls fission in basolateral transport from the Golgi to the plasma membrane and in fluid-phase endocytosis, whereas dynamin is not involved in these steps. Conversely, CtBP3/BARS protein is inactive in apical transport to the plasma membrane and in receptor-mediated endocytosis, both steps being controlled by dynamin. This indicates that CtBP3/BARS controls membrane fission in endocytic and exocytic transport pathways, distinct from those that require dynamin.

KW - Animals

KW - COS Cells

KW - Carrier Proteins

KW - Cell Membrane

KW - Cercopithecus aethiops

KW - Dogs

KW - Dynamins

KW - Endocytosis

KW - Exocytosis

KW - Golgi Apparatus

KW - Intracellular Membranes

KW - Microscopy, Electron, Transmission

KW - Organelles

KW - Protein Transport

KW - Receptors, Cell Surface

KW - Transcription Factors

KW - Transport Vesicles

U2 - 10.1038/ncb1260

DO - 10.1038/ncb1260

M3 - Article

C2 - 15880102

SN - 1465-7392

VL - 7

SP - 570

EP - 580

JO - Nature Cell Biology

JF - Nature Cell Biology

IS - 6

ER -

CtBP3/BARS drives membrane fission in dynamin-independent transport pathways

Abstract

Keywords

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