CXCR4 receptor expression on human retinal pigment epithelial cells from the blood-retina barrier leads to chemokine secretion and migration in response to stromal cell-derived factor 1 alpha

I J Crane, C A Wallace, S McKillop-Smith, J V Forrester

Research output: Contribution to journalArticle

Abstract

Retinal pigment epithelial (RPE) cells form part of the blood-retina barrier and have recently been shown to produce various chemokines in response to proinflammatory cytokines, As the scope of chemokine action has been shown to extend beyond the regulation of leukocyte migration, we have investigated the expression of chemokine receptors on RPE cells to determine whether they could be a target for chemokine signaling. RT-PCR analysis indicated that the predominant receptor expressed on RPE cells was CXCR4. The level of CXCR4 mRNA expression, but not cell surface expression, increased on stimulation,vith It-lp or TNF-alpha. CXCR4 protein could be detected on the surface of 16% of the RPE cells using flow cytometry, Calcium mobilization in response to the CXCR4 ligand stromal cell-derived factor 1 alpha (SDF-1 alpha) indicated that the CXCR4 receptors were functional, Incubation with SDF-1 alpha resulted in secretion of monocyte chemoattractant protein-1, IL-8, and growth-related oncogene iv, RPE cells also migrated in response to SDF-1 alpha. As SDF-1 alpha expression by RPE cells was detected constitutively, we postulate that SDP-1-CXCR4 interactions may modulate the affects of chronic inflammation and subretinal neovascularization at the RPE site of the blood-retina barrier.

Original languageEnglish
Pages (from-to)4372-4378
Number of pages7
JournalThe Journal of Immunology
Volume165
Publication statusPublished - 2000

Keywords

  • ENDOTHELIAL GROWTH-FACTOR
  • NF-KAPPA-B
  • GENE-EXPRESSION
  • LYMPHOCYTE CHEMOATTRACTANT
  • FUNCTIONAL EXPRESSION
  • SURFACE EXPRESSION
  • T-LYMPHOCYTES
  • MESSENGER-RNA
  • HIV-1
  • SDF-1

Cite this

@article{3b5dda57d4e548b7b8c76a311ebd3611,
title = "CXCR4 receptor expression on human retinal pigment epithelial cells from the blood-retina barrier leads to chemokine secretion and migration in response to stromal cell-derived factor 1 alpha",
abstract = "Retinal pigment epithelial (RPE) cells form part of the blood-retina barrier and have recently been shown to produce various chemokines in response to proinflammatory cytokines, As the scope of chemokine action has been shown to extend beyond the regulation of leukocyte migration, we have investigated the expression of chemokine receptors on RPE cells to determine whether they could be a target for chemokine signaling. RT-PCR analysis indicated that the predominant receptor expressed on RPE cells was CXCR4. The level of CXCR4 mRNA expression, but not cell surface expression, increased on stimulation,vith It-lp or TNF-alpha. CXCR4 protein could be detected on the surface of 16{\%} of the RPE cells using flow cytometry, Calcium mobilization in response to the CXCR4 ligand stromal cell-derived factor 1 alpha (SDF-1 alpha) indicated that the CXCR4 receptors were functional, Incubation with SDF-1 alpha resulted in secretion of monocyte chemoattractant protein-1, IL-8, and growth-related oncogene iv, RPE cells also migrated in response to SDF-1 alpha. As SDF-1 alpha expression by RPE cells was detected constitutively, we postulate that SDP-1-CXCR4 interactions may modulate the affects of chronic inflammation and subretinal neovascularization at the RPE site of the blood-retina barrier.",
keywords = "ENDOTHELIAL GROWTH-FACTOR, NF-KAPPA-B, GENE-EXPRESSION, LYMPHOCYTE CHEMOATTRACTANT, FUNCTIONAL EXPRESSION, SURFACE EXPRESSION, T-LYMPHOCYTES, MESSENGER-RNA, HIV-1, SDF-1",
author = "Crane, {I J} and Wallace, {C A} and S McKillop-Smith and Forrester, {J V}",
year = "2000",
language = "English",
volume = "165",
pages = "4372--4378",
journal = "The Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",

}

TY - JOUR

T1 - CXCR4 receptor expression on human retinal pigment epithelial cells from the blood-retina barrier leads to chemokine secretion and migration in response to stromal cell-derived factor 1 alpha

AU - Crane, I J

AU - Wallace, C A

AU - McKillop-Smith, S

AU - Forrester, J V

PY - 2000

Y1 - 2000

N2 - Retinal pigment epithelial (RPE) cells form part of the blood-retina barrier and have recently been shown to produce various chemokines in response to proinflammatory cytokines, As the scope of chemokine action has been shown to extend beyond the regulation of leukocyte migration, we have investigated the expression of chemokine receptors on RPE cells to determine whether they could be a target for chemokine signaling. RT-PCR analysis indicated that the predominant receptor expressed on RPE cells was CXCR4. The level of CXCR4 mRNA expression, but not cell surface expression, increased on stimulation,vith It-lp or TNF-alpha. CXCR4 protein could be detected on the surface of 16% of the RPE cells using flow cytometry, Calcium mobilization in response to the CXCR4 ligand stromal cell-derived factor 1 alpha (SDF-1 alpha) indicated that the CXCR4 receptors were functional, Incubation with SDF-1 alpha resulted in secretion of monocyte chemoattractant protein-1, IL-8, and growth-related oncogene iv, RPE cells also migrated in response to SDF-1 alpha. As SDF-1 alpha expression by RPE cells was detected constitutively, we postulate that SDP-1-CXCR4 interactions may modulate the affects of chronic inflammation and subretinal neovascularization at the RPE site of the blood-retina barrier.

AB - Retinal pigment epithelial (RPE) cells form part of the blood-retina barrier and have recently been shown to produce various chemokines in response to proinflammatory cytokines, As the scope of chemokine action has been shown to extend beyond the regulation of leukocyte migration, we have investigated the expression of chemokine receptors on RPE cells to determine whether they could be a target for chemokine signaling. RT-PCR analysis indicated that the predominant receptor expressed on RPE cells was CXCR4. The level of CXCR4 mRNA expression, but not cell surface expression, increased on stimulation,vith It-lp or TNF-alpha. CXCR4 protein could be detected on the surface of 16% of the RPE cells using flow cytometry, Calcium mobilization in response to the CXCR4 ligand stromal cell-derived factor 1 alpha (SDF-1 alpha) indicated that the CXCR4 receptors were functional, Incubation with SDF-1 alpha resulted in secretion of monocyte chemoattractant protein-1, IL-8, and growth-related oncogene iv, RPE cells also migrated in response to SDF-1 alpha. As SDF-1 alpha expression by RPE cells was detected constitutively, we postulate that SDP-1-CXCR4 interactions may modulate the affects of chronic inflammation and subretinal neovascularization at the RPE site of the blood-retina barrier.

KW - ENDOTHELIAL GROWTH-FACTOR

KW - NF-KAPPA-B

KW - GENE-EXPRESSION

KW - LYMPHOCYTE CHEMOATTRACTANT

KW - FUNCTIONAL EXPRESSION

KW - SURFACE EXPRESSION

KW - T-LYMPHOCYTES

KW - MESSENGER-RNA

KW - HIV-1

KW - SDF-1

M3 - Article

VL - 165

SP - 4372

EP - 4378

JO - The Journal of Immunology

JF - The Journal of Immunology

SN - 0022-1767

ER -