Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells

Pietro Marini, Maria Grazia Cascio, Roger G. Pertwee*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The cyclic AMP assay is a functional assay that is commonly used to determine the pharmacological behavior (agonists, antagonists, and inverse agonists) of G-protein coupled receptor ligands. Here, we describe the cyclic AMP assay that is carried out with commercially available nonradioligand ready-to-use kits and CHO (Chinese Hamster Ovarian) cells stably transfected with the human cannabinoid CB2 receptor.

Original languageEnglish
Title of host publicationEndocannabinoid Signaling.
EditorsM. Maccarrone
Place of PublicationNew York
PublisherHUMANA PRESS INC
Pages171-179
Number of pages9
Volume2576
ISBN (Electronic)978-1-0716-2728-0
ISBN (Print)978-1-0716-2727-3
DOIs
Publication statusPublished - 9 Oct 2023

Publication series

NameMethods in Molecular Biology
Volume2576
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • 3-Isobutyl-1-methylxanthine (IBMX)
  • Agonist
  • Antagonist
  • Cannabinoid receptor type 2
  • Cyclic AMP
  • Forskolin (FSK)
  • Inverse agonist

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