Cyclin A1 shows haplo-insufficiency for normal fertility in male mice.

T. Van Der Meer, W. Y. Chan, L. S. Palazon, Conrad A Nieduszynski, M. Murphy, J. Sobczak-Thépot, M. Carrington, W. H. Colledge

    Research output: Contribution to journalArticle

    25 Citations (Scopus)

    Abstract

    In higher eukaryotes, the cyclins constitute a family of proteins involved in progression through the cell cycle. The cyclin A1 gene (Ccna1) is expressed during meiosis and is required for spermatogenesis. Targeted disruption of the Ccna1 gene with a LacZ reporter gene has allowed us to study the expression pattern of this gene in more detail. We have confirmed expression in mouse pre-meiotic spermatocytes and also detected expression in the accessory olfactory bulb, hippocampus and amygdala of the adult brain. We have also found that the amount of cyclin All protein influences the fertility of male mice and its action is modulated by genetic background. On an outbred genetic background (129S6/SvEv X MF1), Ccna1 (tm1Col) -/- animals are sterile due to spermatogenic arrest prior to the first meiotic division while Ccna1 (Im1Col) +/- mice show reduced sperm production and fertility. This is even more pronounced on an inbred genetic background (129S6/SvEv) where Ccna1 (tm1Col) male mice are sterile due to a severe reduction in the total number of sperm.

    Original languageEnglish
    Pages (from-to)503-511
    Number of pages9
    JournalReproduction
    Volume127
    Issue number4
    DOIs
    Publication statusPublished - Apr 2004

    Keywords

    • GERM-CELLS
    • A1
    • MEIOSIS
    • TRANSCRIPTION
    • MOUSE
    • CYCLE

    Cite this

    Van Der Meer, T., Chan, W. Y., Palazon, L. S., Nieduszynski, C. A., Murphy, M., Sobczak-Thépot, J., ... Colledge, W. H. (2004). Cyclin A1 shows haplo-insufficiency for normal fertility in male mice. Reproduction, 127(4), 503-511. https://doi.org/10.1530/rep.1.00131

    Cyclin A1 shows haplo-insufficiency for normal fertility in male mice. / Van Der Meer, T.; Chan, W. Y.; Palazon, L. S.; Nieduszynski, Conrad A; Murphy, M.; Sobczak-Thépot, J.; Carrington, M.; Colledge, W. H.

    In: Reproduction, Vol. 127, No. 4, 04.2004, p. 503-511.

    Research output: Contribution to journalArticle

    Van Der Meer, T, Chan, WY, Palazon, LS, Nieduszynski, CA, Murphy, M, Sobczak-Thépot, J, Carrington, M & Colledge, WH 2004, 'Cyclin A1 shows haplo-insufficiency for normal fertility in male mice.', Reproduction, vol. 127, no. 4, pp. 503-511. https://doi.org/10.1530/rep.1.00131
    Van Der Meer T, Chan WY, Palazon LS, Nieduszynski CA, Murphy M, Sobczak-Thépot J et al. Cyclin A1 shows haplo-insufficiency for normal fertility in male mice. Reproduction. 2004 Apr;127(4):503-511. https://doi.org/10.1530/rep.1.00131
    Van Der Meer, T. ; Chan, W. Y. ; Palazon, L. S. ; Nieduszynski, Conrad A ; Murphy, M. ; Sobczak-Thépot, J. ; Carrington, M. ; Colledge, W. H. / Cyclin A1 shows haplo-insufficiency for normal fertility in male mice. In: Reproduction. 2004 ; Vol. 127, No. 4. pp. 503-511.
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