Cytokine and growth factor expression in Paget's disease: analysis by reverse-transcription/polymerase chain reaction

S H Ralston, S A Hoey, S J Gallacher, B B Adamson, I T Boyle, Sharon Andrea Gordon

Research output: Contribution to journalArticle

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Abstract

We investigated expression of several cytokines and growth factors in explants of Pagetic and non-Pagetic bone samples using the technique of reverse-transcription/polymerase chain reaction (RT/PCR). Transcripts for IL-1 alpha and IL-1 beta, TNF-alpha, TNF-beta, IL-6, basic fibroblast growth factor (bFGF), transforming growth factor beta (TGF-beta) and insulin-like growth factor-I (IGF-I) were found to a variable degree in both Pagetic and non-Pagetic bone samples, but there was no significant difference in the patterns of expression for these factors in Pagetic bone (n = 18) as compared with non-Pagetic bone (n = 51). There was furthermore, no significant difference in the patterns of expression for the various factors studied when patients were subdivided into mild and severe categories of disease activity using markers of bone formation (serum alkaline phosphatase) or bone resorption (osteoclast counts on adjacent biopsy specimens). Although IL-6 and IL-1 have previously been implicated as bone resorbing factors in Pagetic bone, 40% of our patients with severe disease had not detectable IL-6 transcripts, 70% had no detectable IL-1 alpha transcripts and 50% no IL-1 beta transcripts. We conclude that patterns of expression for cytokine and growth factor mRNAs are not disturbed in Paget's disease. Although we cannot exclude the possibility that post-transcriptional processing of the mRNAs may differ in Pagetic and normal bone cells, our data raise the possibility that the abnormalities of bone turnover which are characteristic of active Paget's disease may be due to local elaboration of other, possibly novel osteotropic factors, which stimulate bone formation and resorption.
Original languageEnglish
Pages (from-to)620-5
Number of pages6
JournalBritish Journal of Rheumatology
Volume33
Issue number7
Publication statusPublished - 1994

Fingerprint

Reverse Transcription
Intercellular Signaling Peptides and Proteins
Cytokines
Bone and Bones
Polymerase Chain Reaction
Interleukin-6
Interleukin-1alpha
Bone Resorption
Interleukin-1beta
Osteogenesis
Lymphotoxin-alpha
Messenger RNA
Bone Remodeling
Fibroblast Growth Factor 2
Osteoclasts
Insulin-Like Growth Factor I
Interleukin-1
Transforming Growth Factor beta
Alkaline Phosphatase
Tumor Necrosis Factor-alpha

Keywords

  • Alkaline Phosphatase
  • Base Sequence
  • Bone and Bones
  • Cytokines
  • DNA
  • Female
  • Fibroblast Growth Factor 2
  • Gene Expression Regulation
  • Growth Substances
  • Humans
  • Insulin-Like Growth Factor I
  • Interleukin-1
  • Interleukin-6
  • Molecular Sequence Data
  • Osteitis Deformans
  • Polymerase Chain Reaction
  • RNA, Messenger
  • Transcription, Genetic
  • Tumor Necrosis Factor-alpha

Cite this

Ralston, S. H., Hoey, S. A., Gallacher, S. J., Adamson, B. B., Boyle, I. T., & Gordon, S. A. (1994). Cytokine and growth factor expression in Paget's disease: analysis by reverse-transcription/polymerase chain reaction. British Journal of Rheumatology, 33(7), 620-5.

Cytokine and growth factor expression in Paget's disease: analysis by reverse-transcription/polymerase chain reaction. / Ralston, S H; Hoey, S A; Gallacher, S J; Adamson, B B; Boyle, I T; Gordon, Sharon Andrea.

In: British Journal of Rheumatology, Vol. 33, No. 7, 1994, p. 620-5.

Research output: Contribution to journalArticle

Ralston, SH, Hoey, SA, Gallacher, SJ, Adamson, BB, Boyle, IT & Gordon, SA 1994, 'Cytokine and growth factor expression in Paget's disease: analysis by reverse-transcription/polymerase chain reaction' British Journal of Rheumatology, vol. 33, no. 7, pp. 620-5.
Ralston, S H ; Hoey, S A ; Gallacher, S J ; Adamson, B B ; Boyle, I T ; Gordon, Sharon Andrea. / Cytokine and growth factor expression in Paget's disease: analysis by reverse-transcription/polymerase chain reaction. In: British Journal of Rheumatology. 1994 ; Vol. 33, No. 7. pp. 620-5.
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abstract = "We investigated expression of several cytokines and growth factors in explants of Pagetic and non-Pagetic bone samples using the technique of reverse-transcription/polymerase chain reaction (RT/PCR). Transcripts for IL-1 alpha and IL-1 beta, TNF-alpha, TNF-beta, IL-6, basic fibroblast growth factor (bFGF), transforming growth factor beta (TGF-beta) and insulin-like growth factor-I (IGF-I) were found to a variable degree in both Pagetic and non-Pagetic bone samples, but there was no significant difference in the patterns of expression for these factors in Pagetic bone (n = 18) as compared with non-Pagetic bone (n = 51). There was furthermore, no significant difference in the patterns of expression for the various factors studied when patients were subdivided into mild and severe categories of disease activity using markers of bone formation (serum alkaline phosphatase) or bone resorption (osteoclast counts on adjacent biopsy specimens). Although IL-6 and IL-1 have previously been implicated as bone resorbing factors in Pagetic bone, 40{\%} of our patients with severe disease had not detectable IL-6 transcripts, 70{\%} had no detectable IL-1 alpha transcripts and 50{\%} no IL-1 beta transcripts. We conclude that patterns of expression for cytokine and growth factor mRNAs are not disturbed in Paget's disease. Although we cannot exclude the possibility that post-transcriptional processing of the mRNAs may differ in Pagetic and normal bone cells, our data raise the possibility that the abnormalities of bone turnover which are characteristic of active Paget's disease may be due to local elaboration of other, possibly novel osteotropic factors, which stimulate bone formation and resorption.",
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AU - Hoey, S A

AU - Gallacher, S J

AU - Adamson, B B

AU - Boyle, I T

AU - Gordon, Sharon Andrea

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N2 - We investigated expression of several cytokines and growth factors in explants of Pagetic and non-Pagetic bone samples using the technique of reverse-transcription/polymerase chain reaction (RT/PCR). Transcripts for IL-1 alpha and IL-1 beta, TNF-alpha, TNF-beta, IL-6, basic fibroblast growth factor (bFGF), transforming growth factor beta (TGF-beta) and insulin-like growth factor-I (IGF-I) were found to a variable degree in both Pagetic and non-Pagetic bone samples, but there was no significant difference in the patterns of expression for these factors in Pagetic bone (n = 18) as compared with non-Pagetic bone (n = 51). There was furthermore, no significant difference in the patterns of expression for the various factors studied when patients were subdivided into mild and severe categories of disease activity using markers of bone formation (serum alkaline phosphatase) or bone resorption (osteoclast counts on adjacent biopsy specimens). Although IL-6 and IL-1 have previously been implicated as bone resorbing factors in Pagetic bone, 40% of our patients with severe disease had not detectable IL-6 transcripts, 70% had no detectable IL-1 alpha transcripts and 50% no IL-1 beta transcripts. We conclude that patterns of expression for cytokine and growth factor mRNAs are not disturbed in Paget's disease. Although we cannot exclude the possibility that post-transcriptional processing of the mRNAs may differ in Pagetic and normal bone cells, our data raise the possibility that the abnormalities of bone turnover which are characteristic of active Paget's disease may be due to local elaboration of other, possibly novel osteotropic factors, which stimulate bone formation and resorption.

AB - We investigated expression of several cytokines and growth factors in explants of Pagetic and non-Pagetic bone samples using the technique of reverse-transcription/polymerase chain reaction (RT/PCR). Transcripts for IL-1 alpha and IL-1 beta, TNF-alpha, TNF-beta, IL-6, basic fibroblast growth factor (bFGF), transforming growth factor beta (TGF-beta) and insulin-like growth factor-I (IGF-I) were found to a variable degree in both Pagetic and non-Pagetic bone samples, but there was no significant difference in the patterns of expression for these factors in Pagetic bone (n = 18) as compared with non-Pagetic bone (n = 51). There was furthermore, no significant difference in the patterns of expression for the various factors studied when patients were subdivided into mild and severe categories of disease activity using markers of bone formation (serum alkaline phosphatase) or bone resorption (osteoclast counts on adjacent biopsy specimens). Although IL-6 and IL-1 have previously been implicated as bone resorbing factors in Pagetic bone, 40% of our patients with severe disease had not detectable IL-6 transcripts, 70% had no detectable IL-1 alpha transcripts and 50% no IL-1 beta transcripts. We conclude that patterns of expression for cytokine and growth factor mRNAs are not disturbed in Paget's disease. Although we cannot exclude the possibility that post-transcriptional processing of the mRNAs may differ in Pagetic and normal bone cells, our data raise the possibility that the abnormalities of bone turnover which are characteristic of active Paget's disease may be due to local elaboration of other, possibly novel osteotropic factors, which stimulate bone formation and resorption.

KW - Alkaline Phosphatase

KW - Base Sequence

KW - Bone and Bones

KW - Cytokines

KW - DNA

KW - Female

KW - Fibroblast Growth Factor 2

KW - Gene Expression Regulation

KW - Growth Substances

KW - Humans

KW - Insulin-Like Growth Factor I

KW - Interleukin-1

KW - Interleukin-6

KW - Molecular Sequence Data

KW - Osteitis Deformans

KW - Polymerase Chain Reaction

KW - RNA, Messenger

KW - Transcription, Genetic

KW - Tumor Necrosis Factor-alpha

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VL - 33

SP - 620

EP - 625

JO - British Journal of Rheumatology

JF - British Journal of Rheumatology

SN - 0263-7103

IS - 7

ER -