Abstract
Gene expression of a number of cytokines in the intestine of rainbow trout (Oncorhynchus mykiss) was investigated after challenge with a pathogenic strain of Aeromonas salmonicida. Fish were exposed to A. salmonicida by immersion in a bacterial suspension (bath challenge) and tissue samples of the distal and proximal intestine were collected at days 0, 2, 4, 6 and 8 post-exposure. Head kidney tissue was also collected to assess the effect in a systemic immune tissue. A classic profile of pro-inflammatory cytokine upregulation was observed in the proximal intestine of fish infected by bath challenge, as determined by semi-quantitative RT-PCR. Expression of IL-1 beta, IL-8, TNF-alpha and IFN-gamma was increased in the proximal intestine. TGF-beta was significantly decreased in the distal intestine. In the head kidney, infection with A. salmonicida by bath challenge caused decreased expression levels of IL-1 beta, IL-8, TNF-alpha and TGF-beta. The results are discussed in the context of potential immune mechanisms in the gut to prevent infection. (c) 2007 Elsevier Ltd. All rights reserved.
Original language | English |
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Pages (from-to) | 747-759 |
Number of pages | 13 |
Journal | Fish & Shellfish Immunology |
Volume | 23 |
Issue number | 4 |
DOIs | |
Publication status | Published - Oct 2007 |
Keywords
- rainbow trout
- intestine
- cytokines
- Aeromonas salmonicida
- salmo-salar l.
- Atlantic salmon
- fugu-rubripes
- recombinant IL-1-beta
- Japanese pufferfish
- vibrio-anguillarum
- defense-mechanisms
- SSP salmonicida
- fish
- interleukin-1-beta