Cytoplasmic reserve polysaccharide of Selenomonas ruminantium

Research output: Contribution to journalArticle

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Abstract

Selenomonas ruminantium accumulated large quantities of intracellular polysaccharide when grown in simple defined medium in a chemostat, particularly at low dilution rate under NH3 limitation when the carbohydrate content of the cells was greater than 40% of the dry weight. This polysaccharide was used as a source of energy under conditions of energy starvation. Abundant, densely staining cytoplasmic granules were observed by electron microscopy in sections stained by the periodic acid-thiocarbohydrazide-osmium technique. The polysaccharide was extracted in 30% KOH followed by precipitation with 60% ethanol and was found to be a glucose homopolymer. Sepharose 4B gel filtration and iodine-complex spectroscopy showed that the polysaccharide was of the glycogen type with a molecular weight of 5 X 10(5) to greater than 20 X 10(5) and an average chain length of 12 glucose residues.
Original languageEnglish
Pages (from-to)630-634
Number of pages5
JournalApplied and Environmental Microbiology
Volume39
Issue number3
Publication statusPublished - Mar 1980

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Selenomonas
Selenomonas ruminantium
polysaccharide
Polysaccharides
polysaccharides
glucose
cytoplasmic granules
Cytoplasmic Granules
Glucose
osmium
chemostat
Periodic Acid
energy
iodine
Starvation
electron microscopy
carbohydrate content
Glycogen
starvation
Iodine

Cite this

Cytoplasmic reserve polysaccharide of Selenomonas ruminantium. / Wallace, R. John.

In: Applied and Environmental Microbiology, Vol. 39, No. 3, 03.1980, p. 630-634.

Research output: Contribution to journalArticle

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