Cytoskeleton organisation during the infection of three brown algal species, Ectocarpus siliculosus, Ectocarpus crouaniorum and Pylaiella littoralis, by the intracellular marine oomycete Eurychasma dicksonii

A. Tsirigoti, F. C. Kuepper, C. M. M. Gachon, C. Katsaros*

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Citations (Scopus)
4 Downloads (Pure)

Abstract

Oomycete diseases in seaweeds are probably widespread and of significant ecological and economic impact, but overall still poorly understood. This study investigates the organisation of the cytoskeleton during infection of three brown algal species, Pylaiella littoralis, Ectocarpus siliculosus, and Ectocarpus crouaniorum, by the basal marine oomycete Eurychasma dicksonii. Immunofluorescence staining of tubulin revealed how the development of this intracellular biotrophic pathogen impacts on microtubule (MT) organisation of its algal host. The host MT cytoskeleton remains normal and organised by the centrosome until very late stages of the infection. Additionally, the organisation of the parasite's cytoskeleton was examined. During mitosis of the E.dicksonii nucleus the MT focal point (microtubule organisation centre, MTOC, putative centrosome) duplicates and each daughter MTOC migrates to opposite poles of the nucleus. This similarity in MT organisation between the host and pathogen reflects the relatively close phylogenetic relationship between oomycetes and brown algae. Moreover, actin labelling with rhodamine-phalloidin in E.dicksonii revealed typical images of actin dots connected by fine actin filament bundles in the cortical cytoplasm. The functional and phylogenetic implications of our observations are discussed.

Original languageEnglish
Pages (from-to)272-281
Number of pages10
JournalPlant Biology
Volume16
Issue number1
Early online date22 May 2013
DOIs
Publication statusPublished - Jan 2014

Keywords

  • actin
  • brown algae
  • cytoskeleton
  • host
  • infection
  • microtubule
  • oomycete
  • phytophthora-cinanmomi
  • microtube organization
  • biotic interactions
  • achyla-bisexualis
  • saprolegnia-ferax
  • life-cycle
  • cell-cycle
  • phaeophyceae
  • zoosporogenesis

Cite this

@article{e2b5f21589fc4d3db75c3a574da2b626,
title = "Cytoskeleton organisation during the infection of three brown algal species, Ectocarpus siliculosus, Ectocarpus crouaniorum and Pylaiella littoralis, by the intracellular marine oomycete Eurychasma dicksonii",
abstract = "Oomycete diseases in seaweeds are probably widespread and of significant ecological and economic impact, but overall still poorly understood. This study investigates the organisation of the cytoskeleton during infection of three brown algal species, Pylaiella littoralis, Ectocarpus siliculosus, and Ectocarpus crouaniorum, by the basal marine oomycete Eurychasma dicksonii. Immunofluorescence staining of tubulin revealed how the development of this intracellular biotrophic pathogen impacts on microtubule (MT) organisation of its algal host. The host MT cytoskeleton remains normal and organised by the centrosome until very late stages of the infection. Additionally, the organisation of the parasite's cytoskeleton was examined. During mitosis of the E.dicksonii nucleus the MT focal point (microtubule organisation centre, MTOC, putative centrosome) duplicates and each daughter MTOC migrates to opposite poles of the nucleus. This similarity in MT organisation between the host and pathogen reflects the relatively close phylogenetic relationship between oomycetes and brown algae. Moreover, actin labelling with rhodamine-phalloidin in E.dicksonii revealed typical images of actin dots connected by fine actin filament bundles in the cortical cytoplasm. The functional and phylogenetic implications of our observations are discussed.",
keywords = "actin, brown algae, cytoskeleton, host, infection, microtubule, oomycete, phytophthora-cinanmomi, microtube organization, biotic interactions, achyla-bisexualis, saprolegnia-ferax, life-cycle, cell-cycle, phaeophyceae, zoosporogenesis",
author = "A. Tsirigoti and Kuepper, {F. C.} and Gachon, {C. M. M.} and C. Katsaros",
note = "Funded by •University of Athens •TOTAL Foundation •European Commission •ASSEMBLE. Grant Number: 227788 •MASTS •Scottish Funding Council. Grant Number: HR09011 •UK NERC. Grant Number: NE/J00460X/1",
year = "2014",
month = "1",
doi = "10.1111/plb.12041",
language = "English",
volume = "16",
pages = "272--281",
journal = "Plant Biology",
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TY - JOUR

T1 - Cytoskeleton organisation during the infection of three brown algal species, Ectocarpus siliculosus, Ectocarpus crouaniorum and Pylaiella littoralis, by the intracellular marine oomycete Eurychasma dicksonii

AU - Tsirigoti, A.

AU - Kuepper, F. C.

AU - Gachon, C. M. M.

AU - Katsaros, C.

N1 - Funded by •University of Athens •TOTAL Foundation •European Commission •ASSEMBLE. Grant Number: 227788 •MASTS •Scottish Funding Council. Grant Number: HR09011 •UK NERC. Grant Number: NE/J00460X/1

PY - 2014/1

Y1 - 2014/1

N2 - Oomycete diseases in seaweeds are probably widespread and of significant ecological and economic impact, but overall still poorly understood. This study investigates the organisation of the cytoskeleton during infection of three brown algal species, Pylaiella littoralis, Ectocarpus siliculosus, and Ectocarpus crouaniorum, by the basal marine oomycete Eurychasma dicksonii. Immunofluorescence staining of tubulin revealed how the development of this intracellular biotrophic pathogen impacts on microtubule (MT) organisation of its algal host. The host MT cytoskeleton remains normal and organised by the centrosome until very late stages of the infection. Additionally, the organisation of the parasite's cytoskeleton was examined. During mitosis of the E.dicksonii nucleus the MT focal point (microtubule organisation centre, MTOC, putative centrosome) duplicates and each daughter MTOC migrates to opposite poles of the nucleus. This similarity in MT organisation between the host and pathogen reflects the relatively close phylogenetic relationship between oomycetes and brown algae. Moreover, actin labelling with rhodamine-phalloidin in E.dicksonii revealed typical images of actin dots connected by fine actin filament bundles in the cortical cytoplasm. The functional and phylogenetic implications of our observations are discussed.

AB - Oomycete diseases in seaweeds are probably widespread and of significant ecological and economic impact, but overall still poorly understood. This study investigates the organisation of the cytoskeleton during infection of three brown algal species, Pylaiella littoralis, Ectocarpus siliculosus, and Ectocarpus crouaniorum, by the basal marine oomycete Eurychasma dicksonii. Immunofluorescence staining of tubulin revealed how the development of this intracellular biotrophic pathogen impacts on microtubule (MT) organisation of its algal host. The host MT cytoskeleton remains normal and organised by the centrosome until very late stages of the infection. Additionally, the organisation of the parasite's cytoskeleton was examined. During mitosis of the E.dicksonii nucleus the MT focal point (microtubule organisation centre, MTOC, putative centrosome) duplicates and each daughter MTOC migrates to opposite poles of the nucleus. This similarity in MT organisation between the host and pathogen reflects the relatively close phylogenetic relationship between oomycetes and brown algae. Moreover, actin labelling with rhodamine-phalloidin in E.dicksonii revealed typical images of actin dots connected by fine actin filament bundles in the cortical cytoplasm. The functional and phylogenetic implications of our observations are discussed.

KW - actin

KW - brown algae

KW - cytoskeleton

KW - host

KW - infection

KW - microtubule

KW - oomycete

KW - phytophthora-cinanmomi

KW - microtube organization

KW - biotic interactions

KW - achyla-bisexualis

KW - saprolegnia-ferax

KW - life-cycle

KW - cell-cycle

KW - phaeophyceae

KW - zoosporogenesis

U2 - 10.1111/plb.12041

DO - 10.1111/plb.12041

M3 - Article

VL - 16

SP - 272

EP - 281

JO - Plant Biology

JF - Plant Biology

SN - 1435-8603

IS - 1

ER -