Declining reactivation ability of Escherichia coli O157 following incubation within soil

A. Prysor Williams*, Helen E. Gordon, Davey L. Jones, Ken Killham, Norval J C Strachan, Ken J. Forbes

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Soil is known to play an important role in the cycling of the human pathogen Escherichia coli O157 in the environment. Here we investigated whether residence time in soil influenced its ability to not only survive but subsequently reactivate after release into water during a simulated rainfall event. We inoculated an agricultural soil with chromosomally lux-marked E.coli O157 and then incubated it at 4 or 15°C for up to 120d, at which point the pathogen was recovered by extracting the soil with rainwater and its reactivation measured by monitoring bioluminescence over a 9h period. We found that with increasing residence time in soil, E.coli O157 exhibited a reduction in both numbers and reactivation capacity as measured by cells' energy status (bioluminescence). As the degree of cell activity is linked to infectivity, this indicates that transmissibility may be reduced after a period within soil. This should be considered when assessing the degree of risk from environmental exposure to bacterial pathogens.

Original languageEnglish
Pages (from-to)85-88
Number of pages4
JournalSoil Biology and Biochemistry
Volume63
Early online date18 Apr 2013
DOIs
Publication statusPublished - Aug 2013

Fingerprint

Escherichia coli O157
reactivation
Soil
incubation
bioluminescence
pathogen
soil
pathogens
residence time
infectivity
rainfall simulation
agricultural soils
rainwater
agricultural soil
Environmental Exposure
pathogenicity
cells
rain
rainfall
monitoring

Keywords

  • Faecal pollution
  • Freshwater
  • Leachate
  • Livestock waste
  • Pathogenicity
  • Runoff
  • Verocytotoxigenic Escherichiacoli (VTEC)

ASJC Scopus subject areas

  • Soil Science
  • Microbiology

Cite this

Declining reactivation ability of Escherichia coli O157 following incubation within soil. / Williams, A. Prysor; Gordon, Helen E.; Jones, Davey L.; Killham, Ken; Strachan, Norval J C; Forbes, Ken J.

In: Soil Biology and Biochemistry, Vol. 63, 08.2013, p. 85-88.

Research output: Contribution to journalArticle

Williams, A. Prysor ; Gordon, Helen E. ; Jones, Davey L. ; Killham, Ken ; Strachan, Norval J C ; Forbes, Ken J. / Declining reactivation ability of Escherichia coli O157 following incubation within soil. In: Soil Biology and Biochemistry. 2013 ; Vol. 63. pp. 85-88.
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abstract = "Soil is known to play an important role in the cycling of the human pathogen Escherichia coli O157 in the environment. Here we investigated whether residence time in soil influenced its ability to not only survive but subsequently reactivate after release into water during a simulated rainfall event. We inoculated an agricultural soil with chromosomally lux-marked E.coli O157 and then incubated it at 4 or 15°C for up to 120d, at which point the pathogen was recovered by extracting the soil with rainwater and its reactivation measured by monitoring bioluminescence over a 9h period. We found that with increasing residence time in soil, E.coli O157 exhibited a reduction in both numbers and reactivation capacity as measured by cells' energy status (bioluminescence). As the degree of cell activity is linked to infectivity, this indicates that transmissibility may be reduced after a period within soil. This should be considered when assessing the degree of risk from environmental exposure to bacterial pathogens.",
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