Decolorization and detoxification of two textile industry effluents by the laccase/1-hydroxybenzotriazole system

Ouafa Benzina, Dalel DAÂSSI, Hela Zouari-Mechichi, Fakher Frikha, Steve Woodward, Lassaad Belbahri, Susana Rodriguez-Couto, Tahar Mechichi

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The aim of this work was to determine the optimal conditions for the decolorization and the detoxification of two effluents from a textile industry—effluent A (the reactive dye bath Bezactive) and effluent B (the direct dye bath Tubantin)—using a laccase mediator system. Response surface methodology (RSM) was applied to optimize textile effluents decolorization. A Box–Behnken design using RSM with the four variables pH, effluent concentration, 1-hydroxybenzotriazole (HBT) concentration, and enzyme (laccase) concentration was used to determine correlations between the effects of these variables on the decolorization of the two effluents. The optimum conditions for pH and concentrations of HBT, effluent and laccase were 5, 1 mM, 50 % and 0.6 U/ml, respectively, for maximum decolorization of effluent A (68 %). For effluent B, optima were 4, 1 mM, 75 %, and 0.6 U/ml, respectively, for maximum decolorization of approximately 88 %. Both effluents were treated at 30 °C for 20 h. A quadratic model was obtained for each decolorization through this design. The experimental and predicted values were in good agreement and both models were highly significant. In addition, the toxicity of the two effluents was determined before and after laccase treatment using Saccharomyces cerevisiae, Bacillus cereus, and germination of tomato seeds.
Original languageEnglish
Pages (from-to)5177-5187
Number of pages11
JournalEnvironmental Science and Pollution Research
Issue number8
Early online date30 Jan 2013
Publication statusPublished - Aug 2013



  • laccase
  • textile effluents
  • Box-Behnken
  • decolorization
  • toxicity

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