Dectin-1 is a major ß-glucan receptor on macrophages

G. D. Brown, P. R. Taylor, D. M. Reid, J. A. Willment, D. Williams, L. Martinez-Pomares, Simon Yuk Chun Wong, S. Gordon

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697 Citations (Scopus)

Abstract

Zymosan is a beta-glucan- and mannan-rich particle that is widely used as a cellular activator for examining the numerous responses effected by phagocytes. The macrophage mannose receptor (MR) and complement receptor 3 (CR3) have historically been considered the major macrophage lectins involved in the nonopsonic recognition of these yeast-derived particles. Using specific carbohydrate inhibitors, we show that a beta-glucan receptor, but not the MR, is a predominant receptor involved in this process. Furthermore, nonopsonic zymosan binding was unaffected by genetic CD11b deficiency or a blocking monoclonal antibody (mAb) against CR3, demonstrating that CR3 was not the beta-glucan receptor mediating this activity. To address the role of the recently described beta-glucan receptor, Dectin-1, we generated a novel anti-Dectin-1 mAb, 2A11. Using this m-Ab, we show here that Dectin-1 was almost exclusively responsible for the beta-glucan-dependent, nonopsonic recognition of zymosan by primary macrophages. These findings define Dectin-1 as the leukocyte beta-glucan receptor, first described over 50 years ago, and resolves the long-standing controversy regarding the identity of this important molecule. Furthermore, these results identify Dectin-1 as a new target for examining the immunomodulatory properties of beta-glucans for therapeutic drug design.

Original languageEnglish
Pages (from-to)407-412
Number of pages5
JournalJournal of Experimental Medicine
Volume196
Issue number3
DOIs
Publication statusPublished - Aug 2002

Keywords

  • lectin
  • macrophage
  • receptor
  • immunology
  • glucans
  • ALTERNATIVE COMPLEMENT PATHWAY
  • MOUSE PERITONEAL-MACROPHAGES
  • BINDING LECTIN SITE
  • MURINE MACROPHAGES
  • HUMAN-MONOCYTES
  • CD11B/CD18
  • ZYMOSAN
  • PHAGOCYTOSIS
  • SPECIFICITY
  • LEUKOCYTES

Cite this

Brown, G. D., Taylor, P. R., Reid, D. M., Willment, J. A., Williams, D., Martinez-Pomares, L., Wong, S. Y. C., & Gordon, S. (2002). Dectin-1 is a major ß-glucan receptor on macrophages. Journal of Experimental Medicine, 196(3), 407-412. https://doi.org/10.1084/jem.20020470