TY - JOUR
T1 - Dectin-1 Is Essential for Reverse Transcytosis of Glycosylated SIgA-Antigen Complexes by Intestinal M Cells
AU - Rochereau, Nicolas
AU - Drocourt, Daniel
AU - Perouzel, Eric
AU - Pavot, Vincent
AU - Redelinghuys, Pierre
AU - Brown, Gordon D.
AU - Tiraby, Gerard
AU - Roblin, Xavier
AU - Verrier, Bernard
AU - Genin, Christian
AU - Corthésy, Blaise
AU - Paul, Stéphane
N1 - Acknowledgments: We express our gratitude to the “Unité Hospitalo-Universitaire d'expérimentation animale” technical platforms of IFR143. We would like also to thank Dr. A. Zimmer.
Funding: NR was supported by a fellowship from the MENRT (France). This work was financed by research grants from the Wellcome Trust (GDB), Sidaction, Cluster 10 of Infectiology, and Cayla-InvivoGen. BC's laboratory is supported by grant no. 3100-138422 from the Swiss Science Research Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
PY - 2013/9/17
Y1 - 2013/9/17
N2 - Intestinal microfold (M) cells possess a high transcytosis capacity and are able to transport a broad range of materials including particulate antigens, soluble macromolecules, and pathogens from the intestinal lumen to inductive sites of the mucosal immune system. M cells are also the primary pathway for delivery of secretory IgA (SIgA) to the gut-associated lymphoid tissue. However, although the consequences of SIgA uptake by M cells are now well known and described, the mechanisms whereby SIgA is selectively bound and taken up remain poorly understood. Here we first demonstrate that both the Cα1 region and glycosylation, more particularly sialic acid residues, are involved in M cell-mediated reverse transcytosis. Second, we found that SIgA is taken up by M cells via the Dectin-1 receptor, with the possible involvement of Siglec-5 acting as a co-receptor. Third, we establish that transcytosed SIgA is taken up by mucosal CX3CR1+ dendritic cells (DCs) via the DC-SIGN receptor. Fourth, we show that mucosal and systemic antibody responses against the HIV p24-SIgA complexes administered orally is strictly dependent on the expression of Dectin-1. Having deciphered the mechanisms leading to specific targeting of SIgA-based Ag complexes paves the way to the use of such a vehicle for mucosal vaccination against various infectious diseases.
AB - Intestinal microfold (M) cells possess a high transcytosis capacity and are able to transport a broad range of materials including particulate antigens, soluble macromolecules, and pathogens from the intestinal lumen to inductive sites of the mucosal immune system. M cells are also the primary pathway for delivery of secretory IgA (SIgA) to the gut-associated lymphoid tissue. However, although the consequences of SIgA uptake by M cells are now well known and described, the mechanisms whereby SIgA is selectively bound and taken up remain poorly understood. Here we first demonstrate that both the Cα1 region and glycosylation, more particularly sialic acid residues, are involved in M cell-mediated reverse transcytosis. Second, we found that SIgA is taken up by M cells via the Dectin-1 receptor, with the possible involvement of Siglec-5 acting as a co-receptor. Third, we establish that transcytosed SIgA is taken up by mucosal CX3CR1+ dendritic cells (DCs) via the DC-SIGN receptor. Fourth, we show that mucosal and systemic antibody responses against the HIV p24-SIgA complexes administered orally is strictly dependent on the expression of Dectin-1. Having deciphered the mechanisms leading to specific targeting of SIgA-based Ag complexes paves the way to the use of such a vehicle for mucosal vaccination against various infectious diseases.
UR - http://www.scopus.com/inward/record.url?scp=84884580565&partnerID=8YFLogxK
U2 - 10.1371/journal.pbio.1001658
DO - 10.1371/journal.pbio.1001658
M3 - Article
C2 - 24068891
AN - SCOPUS:84884580565
VL - 11
SP - 1
EP - 18
JO - PLoS Biology
JF - PLoS Biology
SN - 1544-9173
IS - 9
M1 - e1001658
ER -
